دورية أكاديمية
أعرض في EDS

Complete Biodegradation of Diclofenac by New Bacterial Strains: Postulated Pathways and Degrading Enzymes.

التفاصيل البيبلوغرافية
العنوان: Complete Biodegradation of Diclofenac by New Bacterial Strains: Postulated Pathways and Degrading Enzymes.
المؤلفون: Mohamed MSM; Department of Botany and Microbiology, Faculty of Science, Cairo University, Giza 12613, Egypt., Asair AA; Department of Botany and Microbiology, Faculty of Science, Cairo University, Giza 12613, Egypt., Fetyan NAH; Department of Microbiology, Soil, Water and Environment Research Institute, Agriculture Research Center, Giza 12619, Egypt., Elnagdy SM; Department of Botany and Microbiology, Faculty of Science, Cairo University, Giza 12613, Egypt.
المصدر: Microorganisms [Microorganisms] 2023 May 30; Vol. 11 (6). Date of Electronic Publication: 2023 May 30.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101625893 Publication Model: Electronic Cited Medium: Print ISSN: 2076-2607 (Print) Linking ISSN: 20762607 NLM ISO Abbreviation: Microorganisms Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, [2013]-
مستخلص: The accumulation of xenobiotic compounds in different environments interrupts the natural ecosystem and induces high toxicity in non-target organisms. Diclofenac is one of the commonly used pharmaceutical drugs that persist in the environment due to its low natural degradation rate and high toxicity. Therefore, this study aimed to isolate potential diclofenac-degrading bacteria, detect the intermediate metabolites formed, and determine the enzyme involved in the degradation process. Four bacterial isolates were selected based on their ability to utilize a high concentration of diclofenac (40 mg/L) as the sole carbon source. The growth conditions for diclofenac degradation were optimized, and bacteria were identified as Pseudomonas aeruginosa (S1), Alcaligenes aquatilis (S2), Achromobacter spanius (S11), and Achromobacter piechaudii (S18). The highest percentage of degradation was recorded (97.79 ± 0.84) after six days of incubation for A. spanius S11, as analyzed by HPLC. To detect and identify biodegradation metabolites, the GC-MS technique was conducted for the most efficient bacterial strains. In all tested isolates, the initial hydroxylation of diclofenac was detected. The cleavage step of the NH bridge between the aromatic rings and the subsequent cleavage of the ring adjacent to or in between the two hydroxyl groups of polyhydroxylated derivatives might be a key step that enables the complete biodegradation of diclofenac by A. piechaudii S18, as well as P. aeruginosa S1. Additionally, the laccase, peroxidase, and dioxygenase enzyme activities of the two Achromobacter strains, as well as P. aeruginosa S1, were tested in the presence and absence of diclofenac. The obtained results from this work are expected to be a useful reference for the development of effective detoxification bioprocesses utilizing bacterial cells as biocatalysts. The complete removal of pharmaceuticals from polluted water will stimulate water reuse, meeting the growing worldwide demand for clean and safe freshwater.
References: Water Res. 2013 Apr 1;47(5):1909-18. (PMID: 23384514)
Front Microbiol. 2017 Aug 09;8:1507. (PMID: 28848520)
J Hazard Mater. 2021 Feb 5;403:124000. (PMID: 33265034)
Prostate Int. 2019 Dec;7(4):156-165. (PMID: 31970141)
Ecotoxicol Environ Saf. 2018 May 15;152:104-113. (PMID: 29407776)
Nucleic Acids Res. 2004 Mar 19;32(5):1792-7. (PMID: 15034147)
Environ Pollut. 2018 Sep;240:925-937. (PMID: 29949844)
Mar Pollut Bull. 2015 Jul 15;96(1-2):384-400. (PMID: 25998726)
J Hazard Mater. 2010 Apr 15;176(1-3):836-42. (PMID: 20031320)
Sci Total Environ. 2019 Mar 20;657:739-745. (PMID: 30677939)
AMB Express. 2022 Apr 15;12(1):44. (PMID: 35426615)
Sci Total Environ. 2012 Jul 1;429:123-55. (PMID: 22583809)
Sci Rep. 2019 Jun 24;9(1):9159. (PMID: 31235798)
Water Res. 2011 Jan;45(3):1165-76. (PMID: 21167546)
Sci Total Environ. 2014 Mar 1;473-474:619-41. (PMID: 24394371)
Environ Sci Pollut Res Int. 2020 Jan;27(2):2044-2053. (PMID: 31773532)
Water Res. 2016 Nov 15;105:559-567. (PMID: 27690310)
Environ Pollut. 2020 Oct 20;273:115891. (PMID: 33497943)
Bioresour Technol. 2019 Dec;293:122061. (PMID: 31520862)
Toxicol Lett. 2016 Jun 24;253:46-54. (PMID: 27130197)
Arch Microbiol. 2019 Nov;201(9):1207-1217. (PMID: 31190085)
World J Microbiol Biotechnol. 2021 Jan 20;37(2):33. (PMID: 33469843)
J Chromatogr A. 2013 May 24;1291:19-26. (PMID: 23587315)
AMB Express. 2018 Feb 24;8(1):28. (PMID: 29478084)
Appl Biochem Biotechnol. 2015 Feb;175(3):1294-305. (PMID: 25381650)
Int J Mol Sci. 2020 Sep 16;21(18):. (PMID: 32947916)
J Environ Manage. 2022 Nov 15;322:116098. (PMID: 36081265)
Water Res. 2011 May;45(10):3015-33. (PMID: 21489592)
World J Microbiol Biotechnol. 2011 Apr;27(4):805-811. (PMID: 21475727)
Environ Sci Technol. 2009 Feb 1;43(3):597-603. (PMID: 19244989)
فهرسة مساهمة: Keywords: Achromobacter; Pseudomonas; biodegradation; diclofenac; metabolite
تواريخ الأحداث: Date Created: 20230628 Latest Revision: 20230701
رمز التحديث: 20230701
مُعرف محوري في PubMed: PMC10301663
DOI: 10.3390/microorganisms11061445
PMID: 37374947
قاعدة البيانات: MEDLINE
الوصف
تدمد:2076-2607
DOI:10.3390/microorganisms11061445