دورية أكاديمية
Gene Expression in Synovium of Rotator Cuff Tear Patients Determined by RNA Sequencing.
العنوان: | Gene Expression in Synovium of Rotator Cuff Tear Patients Determined by RNA Sequencing. |
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المؤلفون: | Qian H; Department of Orthopedics, Jinling Hospital, The First School of Clinical Medicine, Southern Medical University, #305, East Zhongshan Road, Nanjing, 210002, China., Meng J; Department of Orthopedics, Jinling Hospital, The First School of Clinical Medicine, Southern Medical University, #305, East Zhongshan Road, Nanjing, 210002, China., Yuan T; Department of Orthopedics, Jinling Hospital, The First School of Clinical Medicine, Southern Medical University, #305, East Zhongshan Road, Nanjing, 210002, China., Jiang H; Department of Orthopedics, Jinling Hospital, The First School of Clinical Medicine, Southern Medical University, #305, East Zhongshan Road, Nanjing, 210002, China., Zhou L; Department of Orthopedics, Jinling Hospital, The First School of Clinical Medicine, Southern Medical University, #305, East Zhongshan Road, Nanjing, 210002, China., Zhang L; Department of Orthopedics, Jinling Hospital, The First School of Clinical Medicine, Southern Medical University, #305, East Zhongshan Road, Nanjing, 210002, China. leizhang1987md@163.com., Zhao J; Department of Orthopedics, Jinling Hospital, The First School of Clinical Medicine, Southern Medical University, #305, East Zhongshan Road, Nanjing, 210002, China. zhaojianning.0207@163.com., Bao N; Department of Orthopedics, Jinling Hospital, The First School of Clinical Medicine, Southern Medical University, #305, East Zhongshan Road, Nanjing, 210002, China. bnrbnr@sina.com. |
المصدر: | Biochemical genetics [Biochem Genet] 2024 Feb; Vol. 62 (1), pp. 452-467. Date of Electronic Publication: 2023 Jun 28. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Kluwer Academic/Plenum Publishers Country of Publication: United States NLM ID: 0126611 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-4927 (Electronic) Linking ISSN: 00062928 NLM ISO Abbreviation: Biochem Genet Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 1999- : New York : Kluwer Academic/Plenum Publishers Original Publication: New York, Plenum Press. |
مواضيع طبية MeSH: | Rotator Cuff Injuries*/genetics , Rotator Cuff Injuries*/surgery , RNA, Long Noncoding*/genetics , RNA, Long Noncoding*/metabolism , MicroRNAs*/genetics, Humans ; Gene Regulatory Networks ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Sequence Analysis, RNA ; Receptors, Interleukin-21/genetics ; Gene Expression ; Fatty Acids |
مستخلص: | Rotator cuff tear (RCT) is a common shoulder disorder related to pain and dysfunction. However, the pathological mechanism of RCT remains unclear. Thus, this study aims to investigate the molecular events in RCT synovium and identify possible target genes and pathways as determined by RNA sequencing (RNA-Seq). The synovial tissue was biopsied from 3 patients with RCT (RCT group) and 3 patients with shoulder instability (Control group) during arthroscopic surgery. Then, differentially expressed (DE) mRNAs, long non-coding RNAs (lncRNAs) and micro RNAs (miRNAs) were comprehensively profiled by RNA-Seq. Gene ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, and competing endogenous RNA (ceRNA) network analysis were performed to identify the potential functions of these DE genes. 447 mRNAs, 103 lncRNAs and 15 miRNAs were identified differentially expressed. The DE mRNAs were highlighted in inflammatory pathway including up-regulated T cell costimulation, positive regulation of T cell activation, and T cell receptor signaling. Down-regulated fatty acid degradation pathway and 5'-AMP-activated protein kinase (AMPK) signaling in RCT group are also enriched. Validation assay showed that the expression of pro-inflammatory molecules including IL21R, CCR5, TNFSF11, and MMP11 was significantly increased in RCT group compared with Control group. CeRNA analysis further revealed lncRNA-miRNA-mRNA regulatory networks involving IL21R and TNFSF11 in RCT. Activated synovial inflammation is the remarkable event of RCT. Importantly, increased T cell activation and disordered fatty acid metabolism signaling might play a significant role. ceRNA networks involving IL21R and TNFSF11 identified could potentially control the progression of RCT. In conclusion, our findings could provide new evidence for the molecular mechanisms of RCT and might identify new therapeutic targets. (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.) |
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معلومات مُعتمدة: | BK20221555 Foundation Research Project of Jiangsu Province; 21XLS18 Research Subject of CMC Logistic Support Department; 81772318 National Natural Science Foundation of China; BE2017723 Natural Science Foundation of Jiangsu Province |
فهرسة مساهمة: | Keywords: Fatty acid degradation; RNA sequencing; Rotator cuff tear; Synovium; T cell signaling |
المشرفين على المادة: | 0 (RNA, Long Noncoding) 0 (MicroRNAs) 0 (RNA, Messenger) 0 (Receptors, Interleukin-21) 0 (Fatty Acids) |
تواريخ الأحداث: | Date Created: 20230628 Date Completed: 20240301 Latest Revision: 20240311 |
رمز التحديث: | 20240311 |
DOI: | 10.1007/s10528-023-10411-y |
PMID: | 37380850 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1573-4927 |
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DOI: | 10.1007/s10528-023-10411-y |