دورية أكاديمية
Generation of vascularized human cardiac organoids for 3D in vitro modeling.
| العنوان: | Generation of vascularized human cardiac organoids for 3D in vitro modeling. |
|---|---|
| المؤلفون: | Voges HK; QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006 Australia; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, VIC 3052 Australia; Department of Paediatrics, School of Medicine, Dentistry and Health Sciences, The University of Melbourne, Melbourne, VIC 3052, Australia; School of Biomedical Sciences, The University of Queensland, Brisbane, QLD 4072, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine, Murdoch Children's Research Institute, Melbourne, VIC 3052, Australia. Electronic address: holly.voges@mcri.edu.au., Mills RJ; QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006 Australia; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, VIC 3052 Australia; Department of Paediatrics, School of Medicine, Dentistry and Health Sciences, The University of Melbourne, Melbourne, VIC 3052, Australia; School of Biomedical Sciences, The University of Queensland, Brisbane, QLD 4072, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine, Murdoch Children's Research Institute, Melbourne, VIC 3052, Australia; School of Biomedical Sciences, Queensland University of Technology, Kelvin Grove, QLD 4000, Australia., Porrello ER; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, VIC 3052 Australia; Novo Nordisk Foundation Center for Stem Cell Medicine, Murdoch Children's Research Institute, Melbourne, VIC 3052, Australia; Department of Anatomy and Physiology, School of Biomedical Sciences, The University of Melbourne, Melbourne, VIC 3052, Australia; Melbourne Centre for Cardiovascular Genomics and Regenerative Medicine, The Royal Children's Hospital, Melbourne, VIC 3052, Australia., Hudson JE; QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006 Australia; School of Biomedical Sciences, The University of Queensland, Brisbane, QLD 4072, Australia. Electronic address: james.hudson@qimrberghofer.edu.au. |
| المصدر: | STAR protocols [STAR Protoc] 2023 Sep 15; Vol. 4 (3), pp. 102371. Date of Electronic Publication: 2023 Jun 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| مواضيع طبية MeSH: | Organoids* , Cell Communication*, Humans ; Cell Differentiation ; Drug Discovery ; Heart |
| مستخلص: | Here, we provide a protocol for next-generation human cardiac organoid modeling containing markers of vascularized tissues. We describe steps for cardiac differentiation, harvesting cardiac cells, and generating vascularized human cardiac organoids. We then detail downstream analysis of functional parameters and fluorescence labeling of human cardiac organoids. This protocol is useful for high throughput disease modeling, drug discovery, and providing mechanistic insight into cell-cell and cell-matrix interactions. For complete details on the use and execution of this protocol, please refer to Voges et al. 1 and Mills et al. 2 . Competing Interests: Declaration of interests R.J.M., J.E.H., and E.R.P. are listed as co-inventors on pending patents that relate to cardiac organoid maturation and cardiac regeneration therapeutics. E.R.P. and J.E.H. are listed as co-inventors on pending patents that relate to endothelial cells in 3D cardiac products. J.E.H. is a co-inventor on licensed patents relating to engineered heart muscle. R.J.M., E.R.P., and J.E.H. are co-founders, scientific advisors, and stockholders in Dynomics. (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Cell Differentiation; Cell culture; Tissue Engineering |
| تواريخ الأحداث: | Date Created: 20230629 Date Completed: 20240308 Latest Revision: 20240308 |
| رمز التحديث: | 20240308 |
| مُعرف محوري في PubMed: | PMC10511847 |
| DOI: | 10.1016/j.xpro.2023.102371 |
| PMID: | 37384522 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2666-1667 |
|---|---|
| DOI: | 10.1016/j.xpro.2023.102371 |