دورية أكاديمية
Isolation and molecular characterization of pathogenic Leptospira species from cattle in Zaria and its environs, Kaduna State, Nigeria.
| العنوان: | Isolation and molecular characterization of pathogenic Leptospira species from cattle in Zaria and its environs, Kaduna State, Nigeria. |
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| المؤلفون: | Udechukwu CC; Department of Veterinary Medicine Ahmadu Bello University, Zaria, Nigeria. Electronic address: sancollinoconsult92@gmail.com., Kudi CA; Department of Veterinary Medicine Ahmadu Bello University, Zaria, Nigeria., Abdu PA; Department of Veterinary Medicine Ahmadu Bello University, Zaria, Nigeria., Mamman PH; Department of Veterinary Microbiology Ahmadu Bello University, Zaria, Nigeria., Pilau NN; Department of Veterinary Medicine Usmanu Danfodiyo University, Sokoto, Nigeria., Abiayi EA; Microbiology Division, Central Diagnostic Laboratory National Veterinary Research Institute, Vom Plateau state, Nigeria., Jolayemie KO; Department of Veterinary Pharmacology and Toxicology, Ahmadu Bello University, Zaria, Nigeria., Okoronkwo MO; Department of Veterinary Pharmacology and Toxicology, Ahmadu Bello University, Zaria, Nigeria., Adekola AA; Harper and Keele Veterinary School, Keele, Newcastle, England, UK; Royal Veterinary College, Pathobiology and Population Sciences, Hawkshead Lane, England, UK. |
| المصدر: | Comparative immunology, microbiology and infectious diseases [Comp Immunol Microbiol Infect Dis] 2023 Aug; Vol. 99, pp. 102003. Date of Electronic Publication: 2023 Jun 21. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 7808924 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1667 (Electronic) Linking ISSN: 01479571 NLM ISO Abbreviation: Comp Immunol Microbiol Infect Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Exeter : Elsevier Science Ltd Original Publication: Oxford, Elmsford, N. Y., Pergamon Press. |
| مواضيع طبية MeSH: | Leptospira* , Leptospirosis*/epidemiology , Leptospirosis*/veterinary, Animals ; Cattle ; Nigeria/epidemiology ; Phylogeny ; Cross-Sectional Studies |
| مستخلص: | Leptospirosis is a zoonotic disease caused by pathogenic serovars of Leptospira spp. The paucity of information on the status of leptospirosis in cattle in the study area led to this study. A cross-sectional study was carried out with a total of 130 kidney samples from cattle which were cultured using Ellinghausen Mc-Cullough Johnson Harris enrichment and examined on dark field microscope after 8 weeks of culture. DNA was extracted directly from 6 kidney tissues to confirm the presence of pathogenic Leptospira spp. and further sequenced to determine the species of Leptospira spp. Result of the culture indicated that the overall occurrence of Leptospira spp.was 32.30 %. Phylogenetic analysis of lipL32 sequences showed Leptospira interrogans isolates from cattle nucleotide homology ranging from 99.40 % to 99.73 % with a query cover of 100 % when compared with sequence from gene bank. In conclusion, this study established that cattle may act as a significant reservoir of leptospirosis in the study area and pose a potential risk to abattoir workers, veterinarians and local communities. Competing Interests: Declaration of Competing Interest All authors hereby disclose no financial or personal relationships that may be perceived as influencing this work. (Copyright © 2023 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Cattle; Culture; Epidemiology; Leptospira; LipL32; Nigeria; PCR |
| تواريخ الأحداث: | Date Created: 20230702 Date Completed: 20230731 Latest Revision: 20230731 |
| رمز التحديث: | 20230731 |
| DOI: | 10.1016/j.cimid.2023.102003 |
| PMID: | 37393647 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1878-1667 |
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| DOI: | 10.1016/j.cimid.2023.102003 |