دورية أكاديمية
Rotenone-exposure as cytofunctional aging model of human dermal fibroblast prior replicative senescence.
| العنوان: | Rotenone-exposure as cytofunctional aging model of human dermal fibroblast prior replicative senescence. |
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| المؤلفون: | da Cruz IBM; Postgraduate Program of Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil; Postgraduate Program of em Gerontology, Federal University of Santa Maria, Santa Maria, RS, Brazil; Open University of the Third Age, Manaus, AM, Brazil., de Afonso Bonotto NC; Postgraduate Program of Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil; Postgraduate Program of em Gerontology, Federal University of Santa Maria, Santa Maria, RS, Brazil., Turra BO; Postgraduate Program of Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil; Open University of the Third Age, Manaus, AM, Brazil., Teixeira CF; Postgraduate Program of Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil., Azzolin VF; Postgraduate Program of em Gerontology, Federal University of Santa Maria, Santa Maria, RS, Brazil; Open University of the Third Age, Manaus, AM, Brazil., Ribeiro EAM; Open University of the Third Age, Manaus, AM, Brazil., Piccoli JDCE; Federal University of Pampa - Campus Uruguaiana, Uruguaiana, Brazil., Barbisan F; Postgraduate Program of Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil; Postgraduate Program of em Gerontology, Federal University of Santa Maria, Santa Maria, RS, Brazil; Open University of the Third Age, Manaus, AM, Brazil. Electronic address: fernandabarbisan@gmail.com. |
| المصدر: | Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2023 Sep; Vol. 91, pp. 105637. Date of Electronic Publication: 2023 Jun 30. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pergamon Press Country of Publication: England NLM ID: 8712158 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3177 (Electronic) Linking ISSN: 08872333 NLM ISO Abbreviation: Toxicol In Vitro Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Oxford ; New York : Pergamon Press, c1987- |
| مواضيع طبية MeSH: | Rotenone*/pharmacology , Cellular Senescence*, Humans ; Aging ; Fibroblasts ; Collagen ; Cells, Cultured |
| مستخلص: | Rotenone (Ro), causes superoxide imbalance by inhibiting complex I of the mitochondrial electron transport chain, being able to serve as a model for functional skin aging by inducing cytofunctional changes in dermal fibroblasts prior to proliferative senescence. To test this hypothesis, we conducted an initial protocol to select a concentration of Ro (0.5, 1, 1.5, 2, 2.5, and 3 μM) that would induce the highest levels of the aging marker beta-galactosidase (β-gal) in human dermal HFF-1 fibroblasts after 72 h of culture, as well as a moderate increase in apoptosis and partial G1 arrestment. We evaluated whether the selected concentration (1 μM) differentially modulated oxidative and cytofunctional markers of fibroblasts. Ro 1.0 μM increased β-gal levels and apoptosis frequency, decreased the frequency of S/G2 cells, induced higher levels of oxidative markers, and presented a genotoxic effect. Fibroblasts exposed to Ro showed lower mitochondrial activity, extracellular collagen deposition, and fewer fibroblast cytoplasmic connections than controls. Ro triggered overexpression of the gene associated with aging (MMP-1), downregulation genes of collagen production (COL1A, FGF-2), and cellular growth/regeneration (FGF-7). The 1 μM concentration of Ro could serve as an experimental model for functional aging fibroblasts prior to replicative senescence. It could be used to identify causal aging mechanisms and strategies to delay skin aging events. Competing Interests: Declaration of Competing Interest The authors declare no conflicts of interest. The authors alone are responsible for the content and writing of the article. (Copyright © 2023 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Collagen metabolism; Oxidative metabolism; Skin aging |
| المشرفين على المادة: | 03L9OT429T (Rotenone) 9007-34-5 (Collagen) |
| تواريخ الأحداث: | Date Created: 20230702 Date Completed: 20230714 Latest Revision: 20230718 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.tiv.2023.105637 |
| PMID: | 37394047 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-3177 |
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| DOI: | 10.1016/j.tiv.2023.105637 |