دورية أكاديمية
أعرض في EDS

Transcriptome profiling of Nudix hydrolase gene deletions in the thermoacidophilic archaeon Sulfolobus acidocaldarius .

التفاصيل البيبلوغرافية
العنوان: Transcriptome profiling of Nudix hydrolase gene deletions in the thermoacidophilic archaeon Sulfolobus acidocaldarius .
المؤلفون: Breuer R; Prokaryotic RNA Biology, Department of Biology, Philipps-Universität Marburg, Marburg, Germany., Gomes-Filho JV; Prokaryotic RNA Biology, Department of Biology, Philipps-Universität Marburg, Marburg, Germany., Yuan J; Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.; SYNMIKRO, Center for Synthetic Microbiology, Marburg, Germany., Randau L; Prokaryotic RNA Biology, Department of Biology, Philipps-Universität Marburg, Marburg, Germany.; SYNMIKRO, Center for Synthetic Microbiology, Marburg, Germany.
المصدر: Frontiers in microbiology [Front Microbiol] 2023 Jun 15; Vol. 14, pp. 1197877. Date of Electronic Publication: 2023 Jun 15 (Print Publication: 2023).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Research Foundation
مستخلص: Nudix hydrolases comprise a large and ubiquitous protein superfamily that catalyzes the hydrolysis of a nucleoside diphosphate linked to another moiety X (Nudix). Sulfolobus acidocaldarius possesses four Nudix domain-containing proteins (SACI_RS00730/Saci_0153, SACI_RS02625/Saci_0550, SACI_RS00060/Saci_0013/Saci_NudT5, and SACI_RS00575/Saci_0121). Deletion strains were generated for the four individual Nudix genes and for both Nudix genes annotated to encode ADP-ribose pyrophosphatases ( SACI_RS00730, SACI_RS00060 ) and did not reveal a distinct phenotype compared to the wild-type strain under standard growth conditions, nutrient stress or heat stress conditions. We employed RNA-seq to establish the transcriptome profiles of the Nudix deletion strains, revealing a large number of differentially regulated genes, most notably in the Δ SACI_RS00730/SACI_RS00060 double knock-out strain and the Δ SACI_RS00575 single deletion strain. The absence of Nudix hydrolases is suggested to impact transcription via differentially regulated transcriptional regulators. We observed downregulation of the lysine biosynthesis and the archaellum formation iModulons in stationary phase cells, as well as upregulation of two genes involved in the de novo NAD + biosynthesis pathway. Furthermore, the deletion strains exhibited upregulation of two thermosome subunits (α, β) and the toxin-antitoxin system VapBC, which are implicated in the archaeal heat shock response. These results uncover a defined set of pathways that involve archaeal Nudix protein activities and assist in their functional characterization.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 Breuer, Gomes-Filho, Yuan and Randau.)
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فهرسة مساهمة: Keywords: Nudix hydrolase; RNA processing; gene regulation; thermophile; transcriptomics
تواريخ الأحداث: Date Created: 20230703 Latest Revision: 20230704
رمز التحديث: 20230704
مُعرف محوري في PubMed: PMC10311068
DOI: 10.3389/fmicb.2023.1197877
PMID: 37396357
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-302X
DOI:10.3389/fmicb.2023.1197877