دورية أكاديمية
Optimizing anesthesia and delivery approaches for dosing into lungs of mice.
| العنوان: | Optimizing anesthesia and delivery approaches for dosing into lungs of mice. |
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| المؤلفون: | Seo Y; Department of Medicine, University of California, San Francisco, California, United States., Qiu L; Department of Medicine, University of California, San Francisco, California, United States., Magnen M; Department of Medicine, University of California, San Francisco, California, United States., Conrad C; Department of Medicine, University of California, San Francisco, California, United States., Moussavi-Harami SF; Department of Medicine, University of California, San Francisco, California, United States., Looney MR; Department of Medicine, University of California, San Francisco, California, United States., Cleary SJ; Department of Medicine, University of California, San Francisco, California, United States. |
| المصدر: | American journal of physiology. Lung cellular and molecular physiology [Am J Physiol Lung Cell Mol Physiol] 2023 Aug 01; Vol. 325 (2), pp. L262-L269. Date of Electronic Publication: 2023 Jul 04. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Physiological Society Country of Publication: United States NLM ID: 100901229 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1504 (Electronic) Linking ISSN: 10400605 NLM ISO Abbreviation: Am J Physiol Lung Cell Mol Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bethesda, MD : American Physiological Society, c1989- |
| مواضيع طبية MeSH: | Anesthesia*/methods , Anesthetics* , Isoflurane* , Ketamine*, Animals ; Mice ; Lung ; Mice, Inbred C57BL ; Reproducibility of Results ; Xylazine |
| مستخلص: | Microbes, toxins, therapeutics, and cells are often instilled into lungs of mice to model diseases and test experimental interventions. Consistent pulmonary delivery is critical for experimental power and reproducibility, but we observed variation in outcomes between handlers using different anesthetic approaches for intranasal dosing in mice. We therefore used a radiotracer to quantify lung delivery after intranasal dosing under inhalational (isoflurane) versus injectable (ketamine/xylazine) anesthesia in C57BL/6 mice. We found that ketamine/xylazine anesthesia resulted in delivery of a greater proportion (52 ± 9%) of an intranasal dose to lungs relative to isoflurane anesthesia (30 ± 15%). This difference in pulmonary dose delivery altered key outcomes in models of viral and bacterial pneumonia, with mice anesthetized with ketamine/xylazine for intranasal infection with influenza A virus or Pseudomonas aeruginosa developing more robust lung inflammation responses relative to control animals randomized to isoflurane anesthesia. Pulmonary dosing efficiency through oropharyngeal aspiration was not affected by anesthetic method and resulted in delivery of 63 ± 8% of dose to lungs, and a nonsurgical intratracheal dosing approach further increased lung delivery to 92 ± 6% of dose. The use of either of these more precise dosing methods yielded greater experimental power in the bacterial pneumonia model relative to intranasal infection. Both anesthetic approach and dosing route can impact pulmonary dosing efficiency. These factors affect experimental power and so should be considered when planning and reporting studies involving delivery of fluids to lungs of mice. NEW & NOTEWORTHY Many lung research studies involve dosing fluids into lungs of mice. In this study, the authors measure lung deposition using intranasal (i.n.), oropharyngeal aspiration (o.a.), and intratracheal (i.t.) dosing methods in mice. Anesthetic approach and administration route were found to affect pulmonary dosing efficiency. The authors demonstrate that refinements to dosing techniques can enable reductions in the number of animals needed for bacterial and viral pneumonia studies. |
| التعليقات: | Update of: bioRxiv. 2023 Jun 29;:. (PMID: 36778478) |
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| معلومات مُعتمدة: | R01 AI160167 United States AI NIAID NIH HHS; R35 HL161241 United States HL NHLBI NIH HHS; T32 HD049303 United States HD NICHD NIH HHS |
| فهرسة مساهمة: | Keywords: dosing; intranasal; intratracheal; lung; mouse |
| المشرفين على المادة: | 0 (Anesthetics) CYS9AKD70P (Isoflurane) 690G0D6V8H (Ketamine) 2KFG9TP5V8 (Xylazine) |
| تواريخ الأحداث: | Date Created: 20230704 Date Completed: 20231002 Latest Revision: 20240210 |
| رمز التحديث: | 20240210 |
| مُعرف محوري في PubMed: | PMC10625824 |
| DOI: | 10.1152/ajplung.00046.2023 |
| PMID: | 37401383 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1522-1504 |
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| DOI: | 10.1152/ajplung.00046.2023 |