دورية أكاديمية
أعرض في EDS

Structural basis of TFIIIC-dependent RNA polymerase III transcription initiation.

التفاصيل البيبلوغرافية
العنوان: Structural basis of TFIIIC-dependent RNA polymerase III transcription initiation.
المؤلفون: Talyzina A; Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA; Interdisciplinary Biological Sciences Program, Northwestern University, Evanston, IL, USA., Han Y; Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA., Banerjee C; Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA., Fishbain S; Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA., Reyes A; Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA; Interdisciplinary Biological Sciences Program, Northwestern University, Evanston, IL, USA., Vafabakhsh R; Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA; Interdisciplinary Biological Sciences Program, Northwestern University, Evanston, IL, USA., He Y; Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA; Interdisciplinary Biological Sciences Program, Northwestern University, Evanston, IL, USA; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA; Robert H. Lurie Comprehensive Cancer Center of Northwestern University, Northwestern University, Chicago, IL, USA. Electronic address: yuanhe@northwestern.edu.
المصدر: Molecular cell [Mol Cell] 2023 Aug 03; Vol. 83 (15), pp. 2641-2652.e7. Date of Electronic Publication: 2023 Jul 03.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 9802571 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4164 (Electronic) Linking ISSN: 10972765 NLM ISO Abbreviation: Mol Cell Subsets: MEDLINE
أسماء مطبوعة: Publication: Cambridge Ma : Cell Press
Original Publication: Cambridge, Mass. : Cell Press, c1997-
مواضيع طبية MeSH: Transcription Factors*/genetics , Transcription Factors*/metabolism , Transcription, Genetic*, Transcription Factor TFIIIB/genetics ; Transcription Factor TFIIIB/metabolism ; RNA Polymerase III/genetics ; RNA Polymerase III/metabolism ; Transcription Factor TFIIIA/genetics ; Transcription Factor TFIIIA/metabolism ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Cryoelectron Microscopy ; DNA/metabolism
مستخلص: RNA polymerase III (Pol III) is responsible for transcribing 5S ribosomal RNA (5S rRNA), tRNAs, and other short non-coding RNAs. Its recruitment to the 5S rRNA promoter requires transcription factors TFIIIA, TFIIIC, and TFIIIB. Here, we use cryoelectron microscopy (cryo-EM) to visualize the S. cerevisiae complex of TFIIIA and TFIIIC bound to the promoter. Gene-specific factor TFIIIA interacts with DNA and acts as an adaptor for TFIIIC-promoter interactions. We also visualize DNA binding of TFIIIB subunits, Brf1 and TBP (TATA-box binding protein), which results in the full-length 5S rRNA gene wrapping around the complex. Our smFRET study reveals that the DNA within the complex undergoes both sharp bending and partial dissociation on a slow timescale, consistent with the model predicted from our cryo-EM results. Our findings provide new insights into the transcription initiation complex assembly on the 5S rRNA promoter and allow us to directly compare Pol III and Pol II transcription adaptations.
Competing Interests: Declaration of interests The authors declare no competing interests.
(Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.)
التعليقات: Update of: bioRxiv. 2023 May 16:2023.05.16.540967. doi: 10.1101/2023.05.16.540967. (PMID: 37292922)
References: Nucleic Acids Res. 2016 Jul 8;44(W1):W344-50. (PMID: 27166375)
IUCrJ. 2020 Feb 11;7(Pt 2):253-267. (PMID: 32148853)
Mol Cell Biol. 1999 Jul;19(7):4944-52. (PMID: 10373544)
Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10822-6. (PMID: 1438283)
Trends Genet. 2008 Dec;24(12):622-9. (PMID: 18980784)
J Biol Chem. 2006 Aug 18;281(33):23945-57. (PMID: 16787917)
Biophys J. 2014 Mar 18;106(6):1327-37. (PMID: 24655508)
Mol Cell. 2006 Oct 20;24(2):221-32. (PMID: 17052456)
Mol Cell Biol. 2002 Jan;22(1):298-308. (PMID: 11739742)
J Biol Chem. 1991 Mar 15;266(8):5162-8. (PMID: 2002052)
Nature. 2018 Jan 17;553(7688):295-300. (PMID: 29345638)
J Struct Biol. 2007 Jan;157(1):38-46. (PMID: 16859925)
Structure. 2012 Feb 8;20(2):205-14. (PMID: 22325770)
J Struct Biol. 2009 Apr;166(1):95-102. (PMID: 19263523)
Cell. 1980 Mar;19(3):717-28. (PMID: 6153931)
J Comput Chem. 2004 Oct;25(13):1605-12. (PMID: 15264254)
Science. 1983 Nov 18;222(4625):740-8. (PMID: 6356356)
J Struct Biol. 2015 Nov;192(2):216-21. (PMID: 26278980)
Nat Methods. 2017 Mar;14(3):290-296. (PMID: 28165473)
Mol Cell Biol. 2004 May;24(10):4118-27. (PMID: 15121834)
Cell. 1985 Jan;40(1):119-27. (PMID: 3967290)
J Biol Chem. 1986 Mar 25;261(9):4309-17. (PMID: 3633269)
J Mol Biol. 1997 Oct 17;273(1):183-206. (PMID: 9367756)
J Struct Biol. 2009 May;166(2):205-13. (PMID: 19374019)
Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15406-11. (PMID: 16227432)
J Mol Biol. 1992 Dec 20;228(4):1063-77. (PMID: 1474578)
Nat Commun. 2020 Sep 30;11(1):4905. (PMID: 32999288)
J Biol Chem. 2000 Aug 25;275(34):26591-8. (PMID: 10859316)
EMBO J. 1989 Dec 1;8(12):3815-24. (PMID: 2684647)
Annu Rev Biochem. 1988;57:873-914. (PMID: 3052292)
Cell Discov. 2018 Jul 31;4:40. (PMID: 30083386)
J Biol Chem. 1995 Jun 23;270(25):15353-8. (PMID: 7797524)
J Vis Exp. 2022 Aug 17;(186):. (PMID: 36062999)
Acta Crystallogr D Struct Biol. 2018 Jun 1;74(Pt 6):531-544. (PMID: 29872004)
J Mol Biol. 2001 Jun 29;310(1):1-26. (PMID: 11419933)
Nature. 2018 Jan 17;553(7688):301-306. (PMID: 29345637)
Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9581-6. (PMID: 11481428)
Proc Natl Acad Sci U S A. 1995 Sep 26;92(20):9338-42. (PMID: 7568129)
Nature. 2021 Aug;596(7873):583-589. (PMID: 34265844)
Mol Cell Biol. 1987 May;7(5):1612-22. (PMID: 3600640)
Commun Biol. 2021 Jul 15;4(1):874. (PMID: 34267316)
Nucleic Acids Res. 2018 Oct 12;46(18):9444-9455. (PMID: 30053100)
Biochemistry. 1992 Nov 24;31(46):11600-5. (PMID: 1332765)
Protein Sci. 2021 Jan;30(1):70-82. (PMID: 32881101)
Mol Cell Biol. 1998 Jan;18(1):1-9. (PMID: 9418847)
J Biol Chem. 1993 May 25;268(15):11364-71. (PMID: 8496187)
J Mol Biol. 1996 Oct 11;262(5):600-14. (PMID: 8876641)
Oncogene. 2004 Apr 19;23(18):3208-16. (PMID: 15094770)
J Biol Chem. 1992 Nov 5;267(31):22562-9. (PMID: 1429605)
Proc Natl Acad Sci U S A. 1993 Jan 1;90(1):168-72. (PMID: 8419919)
Cell. 1992 Dec 11;71(6):1055-64. (PMID: 1458536)
J Biol Chem. 2008 Jul 11;283(28):19184-91. (PMID: 18456653)
J Biol Chem. 2000 Oct 6;275(40):31480-7. (PMID: 10906331)
Biochim Biophys Acta Gene Regul Mech. 2018 Apr;1861(4):320-329. (PMID: 29378333)
Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13442-7. (PMID: 15347814)
J Struct Biol. 2005 Oct;152(1):36-51. (PMID: 16182563)
EMBO J. 1985 Dec 16;4(13A):3473-82. (PMID: 4092686)
Proc Natl Acad Sci U S A. 2000 Nov 7;97(23):12619-24. (PMID: 11058163)
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32. (PMID: 15572765)
Mol Cell Biol. 1991 Oct;11(10):5181-9. (PMID: 1922038)
EMBO J. 1985 Jun;4(6):1609-14. (PMID: 4040853)
Gene. 2022 May 5;821:146282. (PMID: 35149153)
Nucleic Acids Res. 2013 Sep;41(17):8135-43. (PMID: 23856458)
Genes Dev. 1996 Mar 15;10(6):725-39. (PMID: 8598299)
Proc Natl Acad Sci U S A. 1998 Mar 17;95(6):2938-43. (PMID: 9501194)
Biochemistry. 2020 Apr 28;59(16):1565-1581. (PMID: 32216369)
Nucleic Acids Res. 2007;35(14):4869-81. (PMID: 17626045)
J Biol Chem. 1996 May 17;271(20):12103-10. (PMID: 8662611)
Mol Cell Biol. 1989 Jan;9(1):336-41. (PMID: 2927394)
J Struct Biol. 2003 Sep;143(3):185-200. (PMID: 14572474)
Acta Crystallogr D Struct Biol. 2018 Jun 1;74(Pt 6):519-530. (PMID: 29872003)
Cell. 1990 Jan 26;60(2):235-45. (PMID: 2404611)
Mol Cell. 2020 Feb 6;77(3):475-487.e11. (PMID: 31759822)
Trends Genet. 2007 Dec;23(12):614-22. (PMID: 17977614)
Nat Struct Mol Biol. 2010 May;17(5):635-40. (PMID: 20418883)
J Bacteriol. 1978 Apr;134(1):295-305. (PMID: 348684)
Mol Cell Biol. 1997 Dec;17(12):7119-25. (PMID: 9372943)
J Biol Chem. 1989 Apr 15;264(11):6009-12. (PMID: 2703476)
Cell. 2016 Jun 2;165(6):1416-1427. (PMID: 27259150)
J Struct Biol. 2005 Jul;151(1):41-60. (PMID: 15890530)
J Biol Chem. 2013 May 24;288(21):15110-20. (PMID: 23569204)
Cell. 1984 Dec;39(3 Pt 2):479-89. (PMID: 6210149)
Mol Genet Genomics. 2001 Jun;265(4):705-10. (PMID: 11459191)
Nat Struct Biol. 1997 Aug;4(8):605-8. (PMID: 9253405)
Mol Cell Biol. 2006 Aug;26(16):5946-56. (PMID: 16880507)
Mol Cell Biol. 1998 Jan;18(1):420-32. (PMID: 9418889)
Mol Cell Biol. 1989 Jun;9(6):2551-66. (PMID: 2668737)
Nat Commun. 2015 Jun 10;6:7387. (PMID: 26060179)
J Mol Biol. 2006 Mar 17;357(1):275-91. (PMID: 16405997)
Trends Biochem Sci. 2016 Jun;41(6):546-559. (PMID: 27068803)
Proc Natl Acad Sci U S A. 1989 Apr;86(8):2530-4. (PMID: 2649882)
معلومات مُعتمدة: T32 GM140995 United States GM NIGMS NIH HHS; P30 CA060553 United States CA NCI NIH HHS; P01 CA092584 United States CA NCI NIH HHS; R01 GM144559 United States GM NIGMS NIH HHS; U24 GM129547 United States GM NIGMS NIH HHS; R01 GM135651 United States GM NIGMS NIH HHS; R01 GM140272 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: FRET; RNA polymerase III; TFIIIA; TFIIIB; TFIIIC; cryo-EM; transcription
المشرفين على المادة: 0 (transcription factor TFIIIC)
0 (Transcription Factors)
0 (Transcription Factor TFIIIB)
EC 2.7.7.6 (RNA Polymerase III)
0 (Transcription Factor TFIIIA)
9007-49-2 (DNA)
تواريخ الأحداث: Date Created: 20230704 Date Completed: 20230807 Latest Revision: 20240804
رمز التحديث: 20240804
مُعرف محوري في PubMed: PMC10528418
DOI: 10.1016/j.molcel.2023.06.015
PMID: 37402369
قاعدة البيانات: MEDLINE
الوصف
تدمد:1097-4164
DOI:10.1016/j.molcel.2023.06.015