دورية أكاديمية
Structural basis of TFIIIC-dependent RNA polymerase III transcription initiation.
العنوان: | Structural basis of TFIIIC-dependent RNA polymerase III transcription initiation. |
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المؤلفون: | Talyzina A; Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA; Interdisciplinary Biological Sciences Program, Northwestern University, Evanston, IL, USA., Han Y; Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA., Banerjee C; Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA., Fishbain S; Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA., Reyes A; Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA; Interdisciplinary Biological Sciences Program, Northwestern University, Evanston, IL, USA., Vafabakhsh R; Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA; Interdisciplinary Biological Sciences Program, Northwestern University, Evanston, IL, USA., He Y; Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA; Interdisciplinary Biological Sciences Program, Northwestern University, Evanston, IL, USA; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA; Robert H. Lurie Comprehensive Cancer Center of Northwestern University, Northwestern University, Chicago, IL, USA. Electronic address: yuanhe@northwestern.edu. |
المصدر: | Molecular cell [Mol Cell] 2023 Aug 03; Vol. 83 (15), pp. 2641-2652.e7. Date of Electronic Publication: 2023 Jul 03. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural |
اللغة: | English |
بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 9802571 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4164 (Electronic) Linking ISSN: 10972765 NLM ISO Abbreviation: Mol Cell Subsets: MEDLINE |
أسماء مطبوعة: | Publication: Cambridge Ma : Cell Press Original Publication: Cambridge, Mass. : Cell Press, c1997- |
مواضيع طبية MeSH: | Transcription Factors*/genetics , Transcription Factors*/metabolism , Transcription, Genetic*, Transcription Factor TFIIIB/genetics ; Transcription Factor TFIIIB/metabolism ; RNA Polymerase III/genetics ; RNA Polymerase III/metabolism ; Transcription Factor TFIIIA/genetics ; Transcription Factor TFIIIA/metabolism ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Cryoelectron Microscopy ; DNA/metabolism |
مستخلص: | RNA polymerase III (Pol III) is responsible for transcribing 5S ribosomal RNA (5S rRNA), tRNAs, and other short non-coding RNAs. Its recruitment to the 5S rRNA promoter requires transcription factors TFIIIA, TFIIIC, and TFIIIB. Here, we use cryoelectron microscopy (cryo-EM) to visualize the S. cerevisiae complex of TFIIIA and TFIIIC bound to the promoter. Gene-specific factor TFIIIA interacts with DNA and acts as an adaptor for TFIIIC-promoter interactions. We also visualize DNA binding of TFIIIB subunits, Brf1 and TBP (TATA-box binding protein), which results in the full-length 5S rRNA gene wrapping around the complex. Our smFRET study reveals that the DNA within the complex undergoes both sharp bending and partial dissociation on a slow timescale, consistent with the model predicted from our cryo-EM results. Our findings provide new insights into the transcription initiation complex assembly on the 5S rRNA promoter and allow us to directly compare Pol III and Pol II transcription adaptations. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.) |
التعليقات: | Update of: bioRxiv. 2023 May 16:2023.05.16.540967. doi: 10.1101/2023.05.16.540967. (PMID: 37292922) |
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معلومات مُعتمدة: | T32 GM140995 United States GM NIGMS NIH HHS; P30 CA060553 United States CA NCI NIH HHS; P01 CA092584 United States CA NCI NIH HHS; R01 GM144559 United States GM NIGMS NIH HHS; U24 GM129547 United States GM NIGMS NIH HHS; R01 GM135651 United States GM NIGMS NIH HHS; R01 GM140272 United States GM NIGMS NIH HHS |
فهرسة مساهمة: | Keywords: FRET; RNA polymerase III; TFIIIA; TFIIIB; TFIIIC; cryo-EM; transcription |
المشرفين على المادة: | 0 (transcription factor TFIIIC) 0 (Transcription Factors) 0 (Transcription Factor TFIIIB) EC 2.7.7.6 (RNA Polymerase III) 0 (Transcription Factor TFIIIA) 9007-49-2 (DNA) |
تواريخ الأحداث: | Date Created: 20230704 Date Completed: 20230807 Latest Revision: 20240804 |
رمز التحديث: | 20240804 |
مُعرف محوري في PubMed: | PMC10528418 |
DOI: | 10.1016/j.molcel.2023.06.015 |
PMID: | 37402369 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1097-4164 |
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DOI: | 10.1016/j.molcel.2023.06.015 |