دورية أكاديمية
Is domestic polyester suitable for plastination of thin brain slices?
| العنوان: | Is domestic polyester suitable for plastination of thin brain slices? |
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| المؤلفون: | Juvenato LS; Laboratório de Plastinação, Departamento de Morfologia, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.; Programa de Pós-Graduação em Bioquímica, Universidade Federal do Espírito Santo, Vitória, ES, Brasil., Monteiro YF; Laboratório de Plastinação, Departamento de Morfologia, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, ES, Brasil., Miranda RP; Laboratório de Plastinação, Departamento de Morfologia, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, ES, Brasil., Bittencourt APSV; Laboratório de Plastinação, Departamento de Morfologia, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.; Departamento de Ciências Fisiológicas, Universidade Federal do Espírito Santo, Vitória, ES, Brasil., Bittencourt AS; Laboratório de Plastinação, Departamento de Morfologia, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.; Programa de Pós-Graduação em Bioquímica, Universidade Federal do Espírito Santo, Vitória, ES, Brasil. |
| المصدر: | Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas [Braz J Med Biol Res] 2023 Jun 30; Vol. 56, pp. e12566. Date of Electronic Publication: 2023 Jun 30 (Print Publication: 2023). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Associação Brasileira de Divulgação Científica Country of Publication: Brazil NLM ID: 8112917 Publication Model: eCollection Cited Medium: Internet ISSN: 1414-431X (Electronic) Linking ISSN: 0100879X NLM ISO Abbreviation: Braz J Med Biol Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [SP, Brasil : Associação Brasileira de Divulgação Científica, 1981- |
| مواضيع طبية MeSH: | Polyesters* , Plastination*/methods, Animals ; Cattle ; Dehydration ; Polymers ; Brain |
| مستخلص: | Plastination is a technique used to preserve biological tissues while retaining most of their original appearance. In the technique, developed by Dr. Gunther von Hagens in 1977, specimens were impregnated with a polymer, such as silicone, epoxy, or polyester. Considered the most suitable material for brain plastination, polyester has a wide application in teaching and research compared with imaging techniques. The materials for plastination are usually imported from Germany and more expensive than domestic products. If domestic polymers were to enter the market it would favor the expansion of plastination in Brazil. Hence, this study evaluated the feasibility of using domestic polyesters to replace the usual Biodur® (P40) in plastination of brain slices. For this evaluation, 2-mm-thick sections of bovine brains were prepared and plastinated with domestic polyester. Slices were compared before impregnation and after curing using standardized photographs taken after dehydration and after curing. Plastination followed the standard protocol: fixation, dehydration, forced impregnation, and curing. Fifteen brain slices were plastinated with each polyester (P40, P18, and C1-3). There was no significant difference in the percent shrinkage between groups after plastination of P18 and P40, but the curing time of Cristalan© polymer was too short for impregnation. Therefore, no initiator was used for C polymers impregnation. Thus, domestic polyester P18 was a viable option for the process. |
| References: | J Vet Med Educ. 2007 Spring;34(2):172-6. (PMID: 17446645) Anat Embryol (Berl). 1987;175(4):411-21. (PMID: 3555158) Anat Rec. 1979 Jun;194(2):247-55. (PMID: 464325) Foot (Edinb). 2019 Mar;38:34-38. (PMID: 30576888) J Pathol. 1990 Nov;162(3):265-72. (PMID: 2266465) |
| المشرفين على المادة: | 0 (Polyesters) 0 (Polymers) |
| تواريخ الأحداث: | Date Created: 20230705 Date Completed: 20230706 Latest Revision: 20230707 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC10313216 |
| DOI: | 10.1590/1414-431X2023e12566 |
| PMID: | 37403886 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1414-431X |
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| DOI: | 10.1590/1414-431X2023e12566 |