دورية أكاديمية
أعرض في EDS

Gut Colonization by ESBL-Producing Escherichia coli in Dogs Is Associated with a Distinct Microbiome and Resistome Composition.

التفاصيل البيبلوغرافية
العنوان: Gut Colonization by ESBL-Producing Escherichia coli in Dogs Is Associated with a Distinct Microbiome and Resistome Composition.
المؤلفون: Stege PB; Department of Medical Microbiology, UMC Utrecht, Utrecht University, Utrecht, The Netherlands., Hordijk J; Centre for Infectious Disease Control Netherlands, National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.; Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands., Sandholt AKS; Utrecht University, Institute for Risk Assessment Sciences, Utrecht, The Netherlands., Zomer AL; Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.; WHO Collaborating Centre for Reference and Research on Campylobacter and Antimicrobial Resistance from an One Health Perspective/OIE Reference Laboratory for Campylobacteriosis, Utrecht, The Netherlands., Viveen MC; Department of Medical Microbiology, UMC Utrecht, Utrecht University, Utrecht, The Netherlands., Rogers MRC; Department of Medical Microbiology, UMC Utrecht, Utrecht University, Utrecht, The Netherlands., Salomons M; Department of Medical Microbiology, UMC Utrecht, Utrecht University, Utrecht, The Netherlands., Wagenaar JA; Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.; WHO Collaborating Centre for Reference and Research on Campylobacter and Antimicrobial Resistance from an One Health Perspective/OIE Reference Laboratory for Campylobacteriosis, Utrecht, The Netherlands., Mughini-Gras L; Centre for Infectious Disease Control Netherlands, National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.; Utrecht University, Institute for Risk Assessment Sciences, Utrecht, The Netherlands., Willems RJL; Department of Medical Microbiology, UMC Utrecht, Utrecht University, Utrecht, The Netherlands., Paganelli FL; Department of Medical Microbiology, UMC Utrecht, Utrecht University, Utrecht, The Netherlands.
المصدر: Microbiology spectrum [Microbiol Spectr] 2023 Aug 17; Vol. 11 (4), pp. e0006323. Date of Electronic Publication: 2023 Jul 05.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: ASM Press Country of Publication: United States NLM ID: 101634614 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2165-0497 (Electronic) Linking ISSN: 21650497 NLM ISO Abbreviation: Microbiol Spectr Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, DC : ASM Press, 2013-
مواضيع طبية MeSH: Escherichia coli Infections*/microbiology , Gastrointestinal Microbiome*/genetics, Humans ; Dogs ; Animals ; Bacterial Proteins/genetics ; RNA, Ribosomal, 16S/genetics ; Escherichia coli/genetics ; beta-Lactamases/genetics ; Bacteria/genetics ; Feces/microbiology ; Anti-Bacterial Agents/pharmacology
مستخلص: The gut microbiome of humans and animals acts as a reservoir of extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-EC). Dogs are known for having a high prevalence of ESBL-EC in their gut microbiota, although their ESBL-EC carrier status often shifts over time. We hypothesized that the gut microbiome composition of dogs is implicated in ESBL-EC colonization status. Therefore, we assessed whether ESBL-EC carriage in dogs is associated with changes in the gut microbiome and resistome. Fecal samples were collected longitudinally from 57 companion dogs in the Netherlands every 2 weeks for a total of 6 weeks ( n  = 4 samples/dog). Carriage of ESBL-EC was determined through selective culturing and PCR and in line with previous studies, we observed a high prevalence of ESBL-EC carriage in dogs. Using 16s rRNA gene profiling we found significant associations between detected ESBL-EC carriage and an increased abundance of Clostridium sensu stricto 1 , Enterococcus , Lactococcus , and the shared genera of Escherichia -Shigella in the dog microbiome. A resistome capture sequencing approach (ResCap) furthermore, revealed associations between detected ESBL-EC carriage and the increased abundance of the antimicrobial resistance genes: cmlA , dfrA , dhfR , floR , and sul3 . In summary, our study showed that ESBL-EC carriage is associated with a distinct microbiome and resistome composition. IMPORTANCE The gut microbiome of humans and animals is an important source of multidrug resistant pathogens, including beta-lactamase-producing Escherichia coli (ESBL-EC). In this study, we assessed if the carriage of ESBL-EC in dogs was associated with changes in gut composition of bacteria and antimicrobial resistant genes (ARGs). Therefore, stool samples from 57 dogs were collected every 2 weeks for a total of 6 weeks. Sixty eight percent of the dogs carried ESBL-EC during at least one of the time points analyzed. By investigating the gut microbiome and resistome composition, we observed specific changes at time points when dogs were colonized with ESBL-EC compared to time points whenESBL-EC were not detected. In conclusion, our study highlights the importance to study the microbial diversity in companion animals, as gut colonization of particular antimicrobial resistant bacteria might be an indication of a changed microbial composition that is associated with the selection of particular ARGs.
Competing Interests: The authors declare no conflict of interest.
References: PLoS One. 2013 Apr 22;8(4):e61217. (PMID: 23630581)
J Clin Microbiol. 2000 Dec;38(12):4593-8. (PMID: 11101601)
Sci Rep. 2019 Jul 29;9(1):10979. (PMID: 31358818)
J Infect Dev Ctries. 2017 Sep 05;11(8):652-655. (PMID: 31085827)
Front Microbiol. 2013 Aug 16;4:242. (PMID: 23966992)
Nat Rev Microbiol. 2021 Jan;19(1):55-71. (PMID: 32887946)
Antimicrob Resist Infect Control. 2018 Nov 1;7:127. (PMID: 30410748)
ISME J. 2011 Jun;5(6):962-72. (PMID: 21270840)
Nucleic Acids Res. 2020 Jan 8;48(D1):D561-D569. (PMID: 31722416)
Nat Rev Immunol. 2013 Nov;13(11):790-801. (PMID: 24096337)
Environ Microbiol. 2019 Apr;21(4):1331-1343. (PMID: 30680877)
Int J Syst Evol Microbiol. 2017 May;67(5):1393-1399. (PMID: 28126042)
FEMS Microbiol Lett. 2005 Feb 1;243(1):285-91. (PMID: 15668031)
Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. (PMID: 33113351)
Parasitol Res. 2015 Feb;114(2):561-9. (PMID: 25468379)
PeerJ. 2017 Mar 2;5:e3019. (PMID: 28265505)
J Antimicrob Chemother. 2021 Mar 12;76(4):901-908. (PMID: 33428729)
PLoS One. 2018 Aug 15;13(8):e0201279. (PMID: 30110340)
Br J Nutr. 2019 Jul 28;122(2):131-140. (PMID: 30924428)
Cell Host Microbe. 2017 May 10;21(5):592-602.e4. (PMID: 28494240)
New Microbes New Infect. 2016 Nov 28;17:27-29. (PMID: 28275436)
Antimicrob Resist Infect Control. 2020 Jul 14;9(1):107. (PMID: 32665016)
Nat Methods. 2016 Jul;13(7):581-3. (PMID: 27214047)
Bull Environ Contam Toxicol. 2021 Feb;106(2):399-405. (PMID: 33471190)
Antimicrob Agents Chemother. 2015;59(6):3117-24. (PMID: 25779568)
J Antimicrob Chemother. 2020 Feb 1;75(2):342-350. (PMID: 31711228)
FEMS Microbiol Rev. 2004 Nov;28(5):519-42. (PMID: 15539072)
Microbiome. 2014 Feb 24;2(1):6. (PMID: 24558975)
Nat Microbiol. 2020 Mar;5(3):511-524. (PMID: 31988379)
Gut Pathog. 2017 Nov 21;9:68. (PMID: 29201150)
Nat Commun. 2020 Oct 15;11(1):5206. (PMID: 33060586)
Anim Nutr. 2021 Dec;7(4):1189-1204. (PMID: 34754961)
One Health. 2021 Aug 31;13:100322. (PMID: 34522760)
Nucleic Acids Res. 2013 Jan;41(Database issue):D590-6. (PMID: 23193283)
Lancet Microbe. 2022 Jun;3(6):e443-e451. (PMID: 35659906)
Clin Infect Dis. 2020 Nov 5;71(8):1847-1855. (PMID: 31688916)
Water Res. 2014 Jan 1;48:100-7. (PMID: 24091187)
Microbiome. 2018 Jan 15;6(1):11. (PMID: 29335005)
BMC Vet Res. 2017 Feb 28;13(1):65. (PMID: 28245817)
Emerg Infect Dis. 2007 Apr;13(4):559-65. (PMID: 17553270)
Sci Rep. 2021 Sep 16;11(1):18486. (PMID: 34531508)
Microbiologyopen. 2012 Sep;1(3):340-7. (PMID: 23170232)
Nature. 2007 Oct 18;449(7164):804-10. (PMID: 17943116)
Vet Parasitol. 2014 Aug 29;204(3-4):304-9. (PMID: 24880647)
Braz J Microbiol. 2016 Apr-Jun;47(2):337-44. (PMID: 26991286)
Anim Microbiome. 2020 Feb 7;2(1):4. (PMID: 33500005)
J Infect Dis. 2008 Apr 15;197(8):1079-81. (PMID: 18419525)
BMC Bioinformatics. 2018 Aug 29;19(1):307. (PMID: 30157759)
J Microbiol Immunol Infect. 2020 Oct;53(5):757-765. (PMID: 30857922)
Sci Rep. 2022 Feb 3;12(1):1892. (PMID: 35115599)
Nat Biotechnol. 2019 Aug;37(8):852-857. (PMID: 31341288)
Poult Sci. 2021 Jan;100(1):273-282. (PMID: 33357691)
فهرسة مساهمة: Keywords: ESBL; microbiome; resistome
المشرفين على المادة: 0 (Bacterial Proteins)
0 (RNA, Ribosomal, 16S)
EC 3.5.2.6 (beta-Lactamases)
0 (Anti-Bacterial Agents)
تواريخ الأحداث: Date Created: 20230705 Date Completed: 20230821 Latest Revision: 20230824
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10434115
DOI: 10.1128/spectrum.00063-23
PMID: 37404183
قاعدة البيانات: MEDLINE
الوصف
تدمد:2165-0497
DOI:10.1128/spectrum.00063-23