دورية أكاديمية
Precise CRISPR-Cas9-mediated mutation of a membrane trafficking domain in the Drosophila vesicular monoamine transporter gene.
العنوان: | Precise CRISPR-Cas9-mediated mutation of a membrane trafficking domain in the Drosophila vesicular monoamine transporter gene. |
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المؤلفون: | Asuncion JD; Medical Scientist Training Program, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA.; Department of Psychiatry and Biobehavioral Sciences, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA.; UCLA Neuroscience Interdepartmental Program, University of California, Los Angeles, CA, 90095, USA., Eamani A; Department of Psychiatry and Biobehavioral Sciences, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA., Rohrbach EW; Department of Psychiatry and Biobehavioral Sciences, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA.; UCLA Neuroscience Interdepartmental Program, University of California, Los Angeles, CA, 90095, USA., Knapp EM; Department of Psychiatry and Biobehavioral Sciences, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA., Deshpande SA; Department of Psychiatry and Biobehavioral Sciences, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA., Bonanno SL; Department of Psychiatry and Biobehavioral Sciences, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA., Murphy JE; Department of Biological Sciences, Delaware State University, Dover, DE, USA, 19901, USA., Lawal HO; Department of Biological Sciences, Delaware State University, Dover, DE, USA, 19901, USA., Krantz DE; Department of Psychiatry and Biobehavioral Sciences, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA. |
المصدر: | Current research in physiology [Curr Res Physiol] 2023 Jun 20; Vol. 6, pp. 100101. Date of Electronic Publication: 2023 Jun 20 (Print Publication: 2023). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier B.V Country of Publication: Netherlands NLM ID: 101769251 Publication Model: eCollection Cited Medium: Internet ISSN: 2665-9441 (Electronic) Linking ISSN: 26659441 NLM ISO Abbreviation: Curr Res Physiol Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: [Amsterdam] : Elsevier B.V., [2019]- |
مستخلص: | Monoamine neurotransmitters such as noradrenalin are released from both synaptic vesicles (SVs) and large dense-core vesicles (LDCVs), the latter mediating extrasynaptic signaling. The contribution of synaptic versus extrasynaptic signaling to circuit function and behavior remains poorly understood. To address this question, we have previously used transgenes encoding a mutation in the Drosophila Vesicular Monoamine Transporter ( dVMAT ) that shifts amine release from SVs to LDCVs. To circumvent the use of transgenes with non-endogenous patterns of expression, we have now used CRISPR-Cas9 to generate a trafficking mutant in the endogenous dVMAT gene. To minimize disruption of the dVMAT coding sequence and a nearby RNA splice site, we precisely introduced a point mutation using single-stranded oligonucleotide repair. A predicted decrease in fertility was used as a phenotypic screen to identify founders in lieu of a visible marker. Phenotypic analysis revealed a defect in the ovulation of mature follicles and egg retention in the ovaries. We did not detect defects in the contraction of lateral oviducts following optogenetic stimulation of octopaminergic neurons. Our findings suggest that release of mature eggs from the ovary is disrupted by changing the balance of VMAT trafficking between SVs and LDCVs. Further experiments using this model will help determine the mechanisms that sensitize specific circuits to changes in synaptic versus extrasynaptic signaling. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2023 The Authors.) |
References: | Neurology. 2003 Aug 12;61(3):310-5. (PMID: 12913189) Curr Biol. 2014 Mar 31;24(7):725-30. (PMID: 24631243) Proc Natl Acad Sci U S A. 2015 Dec 15;112(50):15480-5. (PMID: 26621748) Genetics. 2020 Oct;216(2):343-352. (PMID: 32817008) Nucleic Acids Res. 2013 Oct;41(19):9049-61. (PMID: 23907390) Elife. 2020 Oct 20;9:. (PMID: 33077027) Neuropharmacology. 2019 Dec 15;161:107564. (PMID: 30851308) Nature. 2016 May 5;533(7601):125-9. (PMID: 27120160) J Neurochem. 2021 Oct;159(1):156-171. (PMID: 34309872) BMC Biol. 2006 Jun 12;4:17. (PMID: 16768790) NPJ Parkinsons Dis. 2021 Mar 5;7(1):22. (PMID: 33674612) Nat Rev Neurosci. 2019 Mar;20(3):177-186. (PMID: 30647451) Nat Methods. 2011 Jun 05;8(7):592-8. (PMID: 21642964) Genetics. 2013 Aug;194(4):1029-35. (PMID: 23709638) Curr Biol. 2015 Feb 16;25(4):467-72. (PMID: 25619767) Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):4562-7. (PMID: 22393023) Front Cell Neurosci. 2022 Jul 22;16:926794. (PMID: 35936490) J Neurochem. 2017 Sep;142(6):857-875. (PMID: 28628197) Dis Model Mech. 2018 Oct 18;11(10):. (PMID: 30355591) Genome Res. 2014 Sep;24(9):1526-33. (PMID: 25096406) J Neurosci. 2003 Sep 17;23(24):8480-8. (PMID: 13679416) J Neurogenet. 2021 Sep;35(3):132-153. (PMID: 33909537) J Biol Chem. 2021 Jan-Jun;296:100787. (PMID: 34015332) Genetics. 2009 Feb;181(2):525-41. (PMID: 19033154) Genetics. 2013 Sep;195(1):289-91. (PMID: 23833182) J Biomed Res. 2020 Aug 21;35(5):339-350. (PMID: 34230437) PLoS One. 2009;4(3):e4716. (PMID: 19262750) Curr Biol. 2015 Mar 16;25(6):751-758. (PMID: 25728694) Front Aging. 2022 Dec 01;3:1068455. (PMID: 36531741) Proc Natl Acad Sci U S A. 2015 Jan 13;112(2):578-83. (PMID: 25548178) Front Physiol. 2012 Jun 06;3:175. (PMID: 22685436) Cell. 2011 Jul 22;146(2):318-31. (PMID: 21757228) PLoS One. 2014 Aug 06;9(8):e104441. (PMID: 25099506) J Biol Chem. 2010 Mar 5;285(10):6867-78. (PMID: 20053989) Fly (Austin). 2014;8(1):52-7. (PMID: 24141137) Dev Biol. 2003 Dec 1;264(1):179-90. (PMID: 14623240) Front Mol Biosci. 2022 Mar 02;9:834498. (PMID: 35295842) Curr Biol. 2015 Sep 21;25(18):2435-40. (PMID: 26344091) J Neurosci. 2018 Jun 6;38(23):5302-5312. (PMID: 29739866) PLoS Genet. 2015 Oct 16;11(10):e1005604. (PMID: 26473732) Mol Psychiatry. 2006 Jan;11(1):99-113. (PMID: 16189511) PLoS One. 2014 Jun 12;9(6):e99732. (PMID: 24923784) Elife. 2013 Apr 16;2:e00415. (PMID: 23599892) Sci Rep. 2013;3:srep02120. (PMID: 23817146) J Exp Biol. 2017 May 15;220(Pt 10):1830-1836. (PMID: 28235907) J Cell Physiol. 2006 Oct;209(1):183-98. (PMID: 16826564) Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):9591-6. (PMID: 24927590) Sci Rep. 2018 Oct 17;8(1):15314. (PMID: 30333565) J Biol Chem. 2005 Apr 15;280(15):14948-55. (PMID: 15691831) J Neurosci. 1999 Jan 1;19(1):72-84. (PMID: 9870940) Sci Rep. 2016 Oct 19;6:35359. (PMID: 27759117) J Cell Biol. 2022 May 2;221(5):. (PMID: 35426896) Prog Neurobiol. 2010 Feb 9;90(2):82-100. (PMID: 19853007) Annu Rev Neurosci. 1997;20:125-56. (PMID: 9056710) Biol Methods Protoc. 2022 Jan 12;7(1):bpac003. (PMID: 35087953) J Neurosci. 2014 May 14;34(20):6924-37. (PMID: 24828646) J Neurochem. 2021 Feb;156(4):445-464. (PMID: 32797733) PLoS Genet. 2008 Nov;4(11):e1000245. (PMID: 18989452) Neurochem Res. 2020 Jun;45(6):1335-1343. (PMID: 32146647) Transl Psychiatry. 2014 Oct 14;4:e464. (PMID: 25313507) Neurochem Res. 2022 Jan;47(1):37-60. (PMID: 33830406) Cell Mol Life Sci. 2012 Feb;69(3):471-84. (PMID: 21818550) J Neurobiol. 2005 Sep 5;64(3):239-58. (PMID: 15849736) Science. 2013 Feb 15;339(6121):819-23. (PMID: 23287718) Biol Proced Online. 2020 Jul 14;22:14. (PMID: 32684853) Cold Spring Harb Perspect Biol. 2012 Feb 01;4(2):. (PMID: 22199021) iScience. 2022 Jul 02;25(8):104697. (PMID: 35880044) |
تواريخ الأحداث: | Date Created: 20230706 Latest Revision: 20230718 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC10318446 |
DOI: | 10.1016/j.crphys.2023.100101 |
PMID: | 37409154 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2665-9441 |
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DOI: | 10.1016/j.crphys.2023.100101 |