دورية أكاديمية
أعرض في EDS

Cell fragmentation in mouse preimplantation embryos induced by ectopic activation of the polar body extrusion pathway.

التفاصيل البيبلوغرافية
العنوان: Cell fragmentation in mouse preimplantation embryos induced by ectopic activation of the polar body extrusion pathway.
المؤلفون: Pelzer D; Institut Curie, PSL Research University, CNRS UMR 3215, INSERM U934, Paris, France., de Plater L; Institut Curie, PSL Research University, CNRS UMR 3215, INSERM U934, Paris, France., Bradbury P; Institut Curie, PSL Research University, CNRS UMR 3215, INSERM U934, Paris, France., Eichmuller A; Institut Curie, PSL Research University, CNRS UMR 3215, INSERM U934, Paris, France., Bourdais A; Institut de Génétique et Développement de Rennes, Université de Rennes, CNRS UMR 6290, Rennes, France., Halet G; Institut de Génétique et Développement de Rennes, Université de Rennes, CNRS UMR 6290, Rennes, France., Maître JL; Institut Curie, PSL Research University, CNRS UMR 3215, INSERM U934, Paris, France.
المصدر: The EMBO journal [EMBO J] 2023 Sep 04; Vol. 42 (17), pp. e114415. Date of Electronic Publication: 2023 Jul 10.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 8208664 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1460-2075 (Electronic) Linking ISSN: 02614189 NLM ISO Abbreviation: EMBO J Subsets: MEDLINE
أسماء مطبوعة: Publication: 2024- : [London] : Nature Publishing Group
Original Publication: Eynsham, Oxford, England : Published for the European Molecular Biology Organization by IRL Press, [c1982-
مواضيع طبية MeSH: Polar Bodies*/metabolism , Actomyosin*/metabolism, Humans ; Animals ; Mice ; Blastocyst ; Chromosomes ; Meiosis ; Oocytes/metabolism ; Spindle Apparatus/genetics ; Myosin Type I/genetics ; Myosin Type I/metabolism
مستخلص: Cell fragmentation is commonly observed in human preimplantation embryos and is associated with poor prognosis during assisted reproductive technology (ART) procedures. However, the mechanisms leading to cell fragmentation remain largely unknown. Here, light sheet microscopy imaging of mouse embryos reveals that inefficient chromosome separation due to spindle defects, caused by dysfunctional molecular motors Myo1c or dynein, leads to fragmentation during mitosis. Extended exposure of the cell cortex to chromosomes locally triggers actomyosin contractility and pinches off cell fragments. This process is reminiscent of meiosis, during which small GTPase-mediated signals from chromosomes coordinate polar body extrusion (PBE) by actomyosin contraction. By interfering with the signals driving PBE, we find that this meiotic signaling pathway remains active during cleavage stages and is both required and sufficient to trigger fragmentation. Together, we find that fragmentation happens in mitosis after ectopic activation of actomyosin contractility by signals emanating from DNA, similar to those observed during meiosis. Our study uncovers the mechanisms underlying fragmentation in preimplantation embryos and, more generally, offers insight into the regulation of mitosis during the maternal-zygotic transition.
(© 2023 The Authors. Published under the terms of the CC BY NC ND 4.0 license.)
References: Curr Top Dev Biol. 2018;128:295-338. (PMID: 29477167)
Nucleic Acids Res. 2018 Jan 4;46(D1):D1266-D1270. (PMID: 29069414)
Nat Cell Biol. 2015 Jul;17(7):849-55. (PMID: 26075357)
J Assist Reprod Genet. 2020 Jun;37(6):1349-1354. (PMID: 32285294)
J Cell Biol. 2007 Jan 29;176(3):295-305. (PMID: 17261848)
Nat Methods. 2010 Mar;7(3):168-9. (PMID: 20195247)
Cell. 2013 Jul 18;154(2):391-402. (PMID: 23870127)
Dev Biol. 2013 May 1;377(1):202-12. (PMID: 23384564)
Mol Biol Cell. 1998 Sep;9(9):2509-25. (PMID: 9725909)
Nat Cell Biol. 2016 Dec;18(12):1302-1310. (PMID: 27842058)
Blood. 2012 Jan 5;119(1):238-50. (PMID: 21908426)
Bioinformatics. 2015 Jan 15;31(2):166-9. (PMID: 25260700)
Nat Struct Mol Biol. 2013 Sep;20(9):1131-9. (PMID: 23934149)
Trends Cell Biol. 2017 Feb;27(2):151-162. (PMID: 27647018)
J Cell Sci. 2019 Apr 26;132(8):. (PMID: 30872458)
Methods Cell Biol. 2017;139:187-201. (PMID: 28215336)
EMBO Rep. 2021 Oct 5;22(10):e52387. (PMID: 34431205)
Rev Reprod. 1999 Sep;4(3):125-34. (PMID: 10521149)
Nature. 2012 Mar 18;484(7392):125-9. (PMID: 22425997)
Genome Res. 2019 Mar;29(3):367-382. (PMID: 30683754)
EMBO J. 2023 Sep 4;42(17):e114415. (PMID: 37427462)
Acta Histochem. 2015 Jan;117(1):111-25. (PMID: 25554607)
Sci Adv. 2020 Apr 03;6(14):eaaz5004. (PMID: 32284983)
Nat Methods. 2012 Jun 28;9(7):676-82. (PMID: 22743772)
Nat Genet. 2017 Jun;49(6):941-945. (PMID: 28459456)
J Cell Sci. 2016 Jul 15;129(14):2689-95. (PMID: 27401928)
Development. 2008 Sep;135(18):3081-91. (PMID: 18725515)
Genesis. 2007 Sep;45(9):593-605. (PMID: 17868096)
Nat Commun. 2012;3:1251. (PMID: 23212380)
Biol Reprod. 1978 Apr;18(3):481-9. (PMID: 667253)
Development. 2008 Feb;135(3):425-9. (PMID: 18094023)
Cell Rep. 2019 Mar 19;26(12):3212-3220.e4. (PMID: 30893595)
Genome Biol. 2014 Feb 03;15(2):R29. (PMID: 24485249)
Fertil Steril. 1999 May;71(5):836-42. (PMID: 10231042)
Cell. 2021 May 27;184(11):2860-2877.e22. (PMID: 33964210)
Fertil Steril. 2011 Mar 15;95(4):1197-204. (PMID: 21146166)
J Cell Biol. 2021 Oct 4;220(10):. (PMID: 34424312)
J Cell Sci. 2018 Nov 22;131(22):. (PMID: 30467138)
J Cell Biol. 2012 Oct 1;199(1):97-110. (PMID: 23027904)
Science. 2018 Nov 23;362(6417):949-952. (PMID: 30467170)
PLoS Biol. 2021 Sep 7;19(9):e3001376. (PMID: 34491981)
Hum Reprod. 2001 Apr;16(4):719-29. (PMID: 11278225)
PLoS Biol. 2022 Mar 24;20(3):e3001593. (PMID: 35324889)
Semin Cell Dev Biol. 2021 Dec;120:22-31. (PMID: 34253437)
Genome Biol. 2013 Apr 25;14(4):R36. (PMID: 23618408)
J Anat. 1978 May;126(Pt 1):209-20. (PMID: 418052)
Nature. 2011 Aug 07;476(7361):462-6. (PMID: 21822289)
Nat Commun. 2022 Nov 8;13(1):6755. (PMID: 36347869)
J Cell Biol. 2021 Dec 6;220(12):. (PMID: 34705028)
Nat Methods. 2008 Jul;5(7):605-7. (PMID: 18536722)
Nucleic Acids Res. 2019 Jul 2;47(W1):W171-W174. (PMID: 31106371)
Nat Cell Biol. 2019 Aug;21(8):966-977. (PMID: 31371827)
Science. 2014 Jan 10;343(6167):193-6. (PMID: 24408435)
Science. 2013 Feb 15;339(6121):819-23. (PMID: 23287718)
Genesis. 2011 Jul;49(7):579-90. (PMID: 21445964)
Dev Cell. 2012 Aug 14;23(2):371-83. (PMID: 22898780)
Nature. 2015 Aug 27;524(7566):489-92. (PMID: 26168397)
Dev Cell. 2007 Feb;12(2):301-8. (PMID: 17276346)
Reprod Biomed Online. 2017 Dec;35(6):640-645. (PMID: 29111314)
فهرسة مساهمة: Keywords: cytoskeleton; meiosis; mitosis; morphogenesis; preimplantation development
المشرفين على المادة: 9013-26-7 (Actomyosin)
0 (Myo1c protein, mouse)
EC 3.6.1.- (Myosin Type I)
تواريخ الأحداث: Date Created: 20230710 Date Completed: 20230905 Latest Revision: 20230906
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10476277
DOI: 10.15252/embj.2023114415
PMID: 37427462
قاعدة البيانات: MEDLINE
الوصف
تدمد:1460-2075
DOI:10.15252/embj.2023114415