دورية أكاديمية
أعرض في EDS

Real-Time, In Situ Imaging of Macrophages via Phase-Change Peptide Nanoemulsions.

التفاصيل البيبلوغرافية
العنوان: Real-Time, In Situ Imaging of Macrophages via Phase-Change Peptide Nanoemulsions.
المؤلفون: Kim I; Department of Biomedical Engineering, Pennsylvania State University, University Park, PA, 16802-4400, USA., Elliott JC; Graduate Program in Acoustics, Pennsylvania State University, University Park, PA, 16802-4400, USA., Lawanprasert A; Department of Biomedical Engineering, Pennsylvania State University, University Park, PA, 16802-4400, USA., Wood GM; Graduate Program in Acoustics, Pennsylvania State University, University Park, PA, 16802-4400, USA., Simon JC; Department of Biomedical Engineering, Pennsylvania State University, University Park, PA, 16802-4400, USA.; Graduate Program in Acoustics, Pennsylvania State University, University Park, PA, 16802-4400, USA., Medina SH; Department of Biomedical Engineering, Pennsylvania State University, University Park, PA, 16802-4400, USA.; Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, 16802-4400, USA.
المصدر: Small (Weinheim an der Bergstrasse, Germany) [Small] 2023 Nov; Vol. 19 (46), pp. e2301673. Date of Electronic Publication: 2023 Jul 14.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101235338 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1613-6829 (Electronic) Linking ISSN: 16136810 NLM ISO Abbreviation: Small Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Weinheim, Germany : Wiley-VCH, c2005-
مواضيع طبية MeSH: Macrophages* , Phagocytes*, Animals ; Swine ; Ultrasonography ; Peptides
مستخلص: Macrophages are specialized phagocytes that play central roles in immunity and tissue repair. Their diverse functionalities have led to an evolution of new allogenic and autologous macrophage products. However, realizing the full therapeutic potential of these cell-based therapies requires development of imaging technologies that can track immune cell migration within tissues in real-time. Such innovations will not only inform treatment regimens and empower interpretation of therapeutic outcomes but also enable prediction and early intervention during adverse events. Here, phase-changing nanoemulsion contrast agents are reported that permit real-time, continuous, and high-fidelity ultrasound imaging of macrophages in situ. Using a de novo designed peptide emulsifier, liquid perfluorocarbon nanoemulsions are prepared and show that rational control over interfacial peptide assembly affords formulations with tunable acoustic sensitivity, macrophage internalization, and in cellulo stability. Imaging experiments demonstrate that emulsion-loaded macrophages can be readily visualized using standard diagnostic B-mode and Doppler ultrasound modalities. This allows on-demand and long-term tracking of macrophages within porcine coronary arteries, as an exemplary model. The results demonstrate that this platform is poised to open new opportunities for non-invasive, contrast-enhanced imaging of cell-based immunotherapies in tissues, while leveraging the low-cost, portable, and safe nature of diagnostic ultrasound.
(© 2023 The Authors. Small published by Wiley-VCH GmbH.)
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معلومات مُعتمدة: R35 GM142902 United States GM NIGMS NIH HHS; R21 DK128638 United States DK NIDDK NIH HHS
فهرسة مساهمة: Keywords: fluorine; macrophage; peptide; self-assembly; ultrasound
المشرفين على المادة: 0 (Peptides)
تواريخ الأحداث: Date Created: 20230715 Date Completed: 20231116 Latest Revision: 20240218
رمز التحديث: 20240218
مُعرف محوري في PubMed: PMC10787802
DOI: 10.1002/smll.202301673
PMID: 37452514
قاعدة البيانات: MEDLINE
الوصف
تدمد:1613-6829
DOI:10.1002/smll.202301673