دورية أكاديمية
أعرض في EDS

Development of ionic liquid-coated PLGA nanoparticles for applications in intravenous drug delivery.

التفاصيل البيبلوغرافية
العنوان: Development of ionic liquid-coated PLGA nanoparticles for applications in intravenous drug delivery.
المؤلفون: Hamadani CM; Department of Chemistry and Biochemistry, University of Mississippi, Oxford, MS, USA., Dasanayake GS; Department of Chemistry and Biochemistry, University of Mississippi, Oxford, MS, USA., Gorniak ME; Department of Chemistry and Biochemistry, University of Mississippi, Oxford, MS, USA., Pride MC; Department of Chemistry and Biochemistry, University of Mississippi, Oxford, MS, USA., Monroe W; Department of Chemistry and Biochemistry, University of Mississippi, Oxford, MS, USA., Chism CM; Department of Chemistry and Biochemistry, University of Mississippi, Oxford, MS, USA., Heintz R; Department of Chemistry and Biochemistry, University of Mississippi, Oxford, MS, USA., Jarrett E; Department of Chemistry and Biochemistry, University of Mississippi, Oxford, MS, USA., Singh G; Department of Chemistry and Biochemistry, University of Mississippi, Oxford, MS, USA., Edgecomb SX; Department of Chemistry and Biochemistry, University of Mississippi, Oxford, MS, USA., Tanner EEL; Department of Chemistry and Biochemistry, University of Mississippi, Oxford, MS, USA. eetanner@olemiss.edu.
المصدر: Nature protocols [Nat Protoc] 2023 Aug; Vol. 18 (8), pp. 2509-2557. Date of Electronic Publication: 2023 Jul 19.
نوع المنشور: Journal Article; Review; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101284307 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1750-2799 (Electronic) Linking ISSN: 17502799 NLM ISO Abbreviation: Nat Protoc Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London, UK : Nature Pub. Group, 2006-
مواضيع طبية MeSH: Ionic Liquids* , Nanoparticles*/chemistry, Mice ; Animals ; Polylactic Acid-Polyglycolic Acid Copolymer ; Polyglycolic Acid/chemistry ; Lactic Acid ; Tissue Distribution ; Drug Carriers/chemistry
مستخلص: Polymeric nanoparticles (NPs) are a promising platform for medical applications in drug delivery. However, their use as drug carriers is limited by biological (e.g., immunological) barriers after intravenous administration. Ionic liquids (ILs), formed from bulky asymmetric cations and anions, have a wide variety of physical internal and external interfacing properties. When assembled on polymeric NPs as biomaterial coatings, these external-interfacing properties can be tuned to extend their circulation half-life when intravenously injected, as well as drive biodistribution to sites of interest for selective organ accumulation. In our work, we are particularly interested in optimizing IL coatings to enable red blood cell hitchhiking in whole blood. In this protocol, we describe the preparation and physicochemical and biological characterization of choline carboxylate IL-coated polymeric NPs. The procedure is divided into five stages: (1) synthesis and characterization of choline-based ILs (1 week); (2) bare poly(lactic-co-glycolic acid) (50:50, acid terminated) Resomer 504H (PLGA) NP assembly, modified from previously established protocols, with dye encapsulation (7 h); (3) modification of the bare particles with IL coating (3 h); (4) physicochemical characterization of both PLGA and IL-PLGA NPs by dynamic light scattering, 1 H nuclear magnetic resonance spectroscopy, and transmission electron microscopy (1 week); (5) ex vivo evaluation of intravenous biocompatibility (including serum-protein resistance and hemolysis) and red blood cell hitchhiking in whole BALB/c mouse blood via fluorescence-activated cell sorting (1 week). With practice and technique refinement, this protocol is accessible to late-stage graduate students and early-stage postdoctoral scientists.
(© 2023. Springer Nature Limited.)
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المشرفين على المادة: 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
0 (Ionic Liquids)
26009-03-0 (Polyglycolic Acid)
33X04XA5AT (Lactic Acid)
0 (Drug Carriers)
تواريخ الأحداث: Date Created: 20230719 Date Completed: 20230809 Latest Revision: 20230812
رمز التحديث: 20231215
DOI: 10.1038/s41596-023-00843-6
PMID: 37468651
قاعدة البيانات: MEDLINE
الوصف
تدمد:1750-2799
DOI:10.1038/s41596-023-00843-6