دورية أكاديمية
The identification of diurnal variations on circulating immune cells by finger prick blood sampling in small sample sizes: a pilot study.
| العنوان: | The identification of diurnal variations on circulating immune cells by finger prick blood sampling in small sample sizes: a pilot study. |
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| المؤلفون: | Bushell D; Faculty of Health Sciences and Medicine, Bond University, Robina, Australia., Tan JKH; Clem Jones Centre for Regenerative Medicine, Bond University, Robina, Australia., Smith J; Clem Jones Centre for Regenerative Medicine, Bond University, Robina, Australia., Moro C; Faculty of Health Sciences and Medicine, Bond University, Robina, Australia. |
| المصدر: | Laboratory medicine [Lab Med] 2024 Mar 07; Vol. 55 (2), pp. 220-226. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 0250641 Publication Model: Print Cited Medium: Internet ISSN: 1943-7730 (Electronic) Linking ISSN: 00075027 NLM ISO Abbreviation: Lab Med Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oct. 2015- : Oxford, England: Oxford University Press Original Publication: Philadelphia, Lippincott. |
| مواضيع طبية MeSH: | T-Lymphocytes* , Killer Cells, Natural*, Humans ; Pilot Projects ; Sample Size ; Leukocytes ; Flow Cytometry |
| مستخلص: | Objective: There are well-described impacts of biological rhythms on human physiology. With the increasing push for routine blood tests for preventative medical care and clinical and physiological research, optimizing effectiveness is paramount. This study aimed to determine whether it is feasible to assess diurnal variations of peripheral lymphocyte prevalence using finger prick blood in a small sample size. Methods: Using polychromatic flow cytometry, the prevalence of lymphocytes was assessed using 25 µL fingertip blood samples at 8 AM and 5 PM from 8 participants. Results: TH cells and B cells showed significantly higher percentages in the 5 PM samples, whereas NK cells demonstrated a significantly higher morning percentage. T cells, leukocytes, and cytotoxic T cells showed no significant changes. Conclusion: The detection of diurnal variations demonstrates that small blood volumes can be used to detect lymphocyte variations. The lower blood volume required provides a new testing method for clinical and research settings. (© The Author(s) 2023. Published by Oxford University Press on behalf of American Society for Clinical Pathology.) |
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| فهرسة مساهمة: | Keywords: T cells; blood; clinical; lymphocytes; physiology; variations |
| تواريخ الأحداث: | Date Created: 20230721 Date Completed: 20240311 Latest Revision: 20240311 |
| رمز التحديث: | 20240311 |
| مُعرف محوري في PubMed: | PMC10920974 |
| DOI: | 10.1093/labmed/lmad062 |
| PMID: | 37478465 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1943-7730 |
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| DOI: | 10.1093/labmed/lmad062 |