دورية أكاديمية
[Survey of Helicobacter pylori levofloxacin and clarithromycin resistance rates and drug resistance genes in Ningxia, 2020-2022].
| العنوان: | [Survey of Helicobacter pylori levofloxacin and clarithromycin resistance rates and drug resistance genes in Ningxia, 2020-2022]. |
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| المؤلفون: | Du LM; Department of Gastroenterology, the Third Clinical Medical College of Ningxia Medical University, Ningxia Hui Autonomous Region People's Hospital, Yinchuan 750002, China., Hu SJ; Department of Gastroenterology, the Third Clinical Medical College of Ningxia Medical University, Ningxia Hui Autonomous Region People's Hospital, Yinchuan 750002, China., Chen XM; Department of Gastroenterology, the Third Clinical Medical College of Ningxia Medical University, Ningxia Hui Autonomous Region People's Hospital, Yinchuan 750002, China., Deng YY; Department of Gastroenterology, the Third Clinical Medical College of Ningxia Medical University, Ningxia Hui Autonomous Region People's Hospital, Yinchuan 750002, China., Yong HL; Department of Gastroenterology, the Third Clinical Medical College of Ningxia Medical University, Ningxia Hui Autonomous Region People's Hospital, Yinchuan 750002, China., Shi RC; Department of Gastroenterology, Wuzhong People's Hospital, Wuzhong 751199, China., Liu JG; Department of Gastroenterology, the Third Clinical Medical College of Ningxia Medical University, Ningxia Hui Autonomous Region People's Hospital, Yinchuan 750002, China., Cao ZZ; Department of Gastroenterology, the Third Clinical Medical College of Ningxia Medical University, Ningxia Hui Autonomous Region People's Hospital, Yinchuan 750002, China., You YJ; Department of Gastroenterology, the Third Clinical Medical College of Ningxia Medical University, Ningxia Hui Autonomous Region People's Hospital, Yinchuan 750002, China., Liu YX; Department of Gastroenterology, the Third Clinical Medical College of Ningxia Medical University, Ningxia Hui Autonomous Region People's Hospital, Yinchuan 750002, China., Ma SC; Department of Gastroenterology, the Third Clinical Medical College of Ningxia Medical University, Ningxia Hui Autonomous Region People's Hospital, Yinchuan 750002, China., Ma LK; Department of Gastroenterology, the Third Clinical Medical College of Ningxia Medical University, Ningxia Hui Autonomous Region People's Hospital, Yinchuan 750002, China., Li XF; Department of Gastroenterology, the Third Clinical Medical College of Ningxia Medical University, Ningxia Hui Autonomous Region People's Hospital, Yinchuan 750002, China., Li XM; Department of Gastroenterology, the Third Clinical Medical College of Ningxia Medical University, Ningxia Hui Autonomous Region People's Hospital, Yinchuan 750002, China., Hou JB; Department of Gastroenterology, the Third Clinical Medical College of Ningxia Medical University, Ningxia Hui Autonomous Region People's Hospital, Yinchuan 750002, China., Ye ZC; Department of Gastroenterology, the Third Clinical Medical College of Ningxia Medical University, Ningxia Hui Autonomous Region People's Hospital, Yinchuan 750002, China., Sang T; Department of Gastroenterology, the Third Clinical Medical College of Ningxia Medical University, Ningxia Hui Autonomous Region People's Hospital, Yinchuan 750002, China., Cao Y; Department of Gastroenterology, the Third Clinical Medical College of Ningxia Medical University, Ningxia Hui Autonomous Region People's Hospital, Yinchuan 750002, China., Liu H; Department of Gastroenterology, the Third Clinical Medical College of Ningxia Medical University, Ningxia Hui Autonomous Region People's Hospital, Yinchuan 750002, China., Wei XX; Department of Gastroenterology, the Third Clinical Medical College of Ningxia Medical University, Ningxia Hui Autonomous Region People's Hospital, Yinchuan 750002, China., Hu AL; Department of Gastroenterology, the Third Clinical Medical College of Ningxia Medical University, Ningxia Hui Autonomous Region People's Hospital, Yinchuan 750002, China., Li YL; Department of Gastroenterology, the Third Clinical Medical College of Ningxia Medical University, Ningxia Hui Autonomous Region People's Hospital, Yinchuan 750002, China., Gao HJ; Department of Gastroenterology, Tongji Hospital, Tongji University, Institute of Digestive Disease, School of Medicine, Tongji University, Shanghai 200120, China. |
| المصدر: | Zhonghua yi xue za zhi [Zhonghua Yi Xue Za Zhi] 2023 Jul 25; Vol. 103 (28), pp. 2163-2167. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Zhonghua yi xue hui Country of Publication: China NLM ID: 7511141 Publication Model: Print Cited Medium: Print ISSN: 0376-2491 (Print) Linking ISSN: 03762491 NLM ISO Abbreviation: Zhonghua Yi Xue Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Beijing : Zhonghua yi xue hui Original Publication: Beijing : Zhonghua yi xue hui. |
| مواضيع طبية MeSH: | Helicobacter Infections* , Helicobacter pylori*/genetics, Female ; Humans ; Male ; Anti-Bacterial Agents/pharmacology ; Clarithromycin/pharmacology ; Cross-Sectional Studies ; Drug Resistance, Bacterial/genetics ; Levofloxacin/pharmacology ; Microbial Sensitivity Tests ; Retrospective Studies ; Adolescent ; Young Adult ; Adult ; Middle Aged ; Aged ; Aged, 80 and over |
| مستخلص: | Objective: To explore the rate of Helicobacter pylori (Hp) resistance to levofloxacin and clarithromycin and the common mutation patterns of resistance genes in Ningxia, and to assess the concordance between phenotypic resistance and genotypic resistance. Methods: Cross-sectional study. Patients diagnosed with Hp infection in 14 hospitals in Ningxia region from February 2020 to May 2022 were retrospectively selected. Hp strains were isolated from gastric biopsy specimens of Hp-infected patients and subjected to phenotypic drug sensitivity testing and detection of resistance genes to analyze the rate of Hp resistance to levofloxacin and clarithromycin and the common mutation patterns of resistance genes in Ningxia region; and the concordance rate and Kappa concordance test were used to assess the concordance between phenotypic resistance and genotypic resistance. Results: A total of 1 942 Hp strains were isolated and cultured, and among the infections, 1 069 cases (55.0%) were male and 873 cases (45.0%) were female, aged (50.0±12.5) years (15-86 years). The rates of Hp resistance to levofloxacin and clarithromycin in Ningxia were 42.1% (818/1 942) and 40.1% (779/1 942), respectively, and the rate of dual resistance to both was 22.8% (443/1 942). The rate of resistance to levofloxacin and clarithromycin of Hp strains from female patients was higher than in male patients (levofloxacin: 50.4%(440/873) vs 35.4%(378/1 069); clarithromycin: 44.4%(388/873) vs 36.6%(391/1 069), both P <0.001). Among the GyrA gene mutations associated with levofloxacin resistance, the differences in mutation rate of amino acid at positions 87 and 91 were statistically significant in both drug-resistant and sensitive strains(both P <0.001), except for Asn87Thr. Hp strains were statistically significant for levofloxacin (Kappa=0.834, P <0.001) and clarithromycin (Kappa=0.829, P <0.001) had good concordance in resistance at the phenotypic and genotypic levels. Conclusion: The resistance of Hp to levofloxacin and clarithromycin in Ningxia region is severe, and there is good consistency between genotypic and phenotypic resistance. |
| معلومات مُعتمدة: | 2019BFG02003, 2021BEG02025 Ningxia Hui Autonomous Region Key Research and Development Program; 2022CMG03039 Ningxia Hui Autonomous Region Science and Technology Special Project to Benefit the People |
| فهرسة مساهمة: | Local Abstract: [Publisher, Chinese] 目的： 探索宁夏地区幽门螺杆菌（Hp）对左氧氟沙星和克拉霉素耐药率及耐药基因的常见突变方式，评估其表型与基因型耐药的一致性。 方法： 横断面研究。回顾性选取2020年2月至2022年5月宁夏地区14家医院确诊为Hp感染的患者。从Hp感染患者的胃活检标本中分离到Hp菌株，进行表型药敏试验和耐药基因的检测，分析宁夏地区Hp对左氧氟沙星和克拉霉素耐药率及耐药基因常见突变方式；采用符合率和Kappa系数评估表型与基因型耐药的一致性。 结果： 共分离培养1 942株Hp，感染者中，男1 069例（55.0%），女873例（45.0%），年龄（50.0±12.5）岁（15~86岁）。宁夏地区Hp对左氧氟沙星和克拉霉素耐药率分别为42.1%（818/1 942）和40.1%（779/1 942），双重耐药率为22.8%（443/1 942）。其中来自女性患者的Hp菌株对左氧氟沙星和克拉霉素耐药率均较男性高（左氧氟沙星：50.4%（440/873）比35.4%（378/1 069）；克拉霉素：44.4%（388/873）比36.6%（391/1 069），均 P <0.001）。与左氧氟沙星耐药有关的GyrA基因突变中，除了Asn87Thr外，第87和91位氨基酸突变率在耐药菌株和敏感菌株间的差异均有统计学意义（均 P <0.001）。Hp菌株对左氧氟沙星（Kappa=0.834， P <0.001）和克拉霉素（Kappa=0.829， P <0.001）的耐药性在表型和基因型上具有良好的一致性。 结论： 宁夏地区Hp对左氧氟沙星和克拉霉素的耐药严重，基因型和表型耐药具有良好的一致性。. |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) H1250JIK0A (Clarithromycin) 6GNT3Y5LMF (Levofloxacin) |
| تواريخ الأحداث: | Date Created: 20230724 Date Completed: 20230801 Latest Revision: 20230801 |
| رمز التحديث: | 20240628 |
| DOI: | 10.3760/cma.j.cn112137-20230214-00206 |
| PMID: | 37482728 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0376-2491 |
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| DOI: | 10.3760/cma.j.cn112137-20230214-00206 |