دورية أكاديمية
Effect of hypoandrogenism on expression of transient receptor potential vanilloid channels in rat penile corpus cavernosum and erectile function.
| العنوان: | Effect of hypoandrogenism on expression of transient receptor potential vanilloid channels in rat penile corpus cavernosum and erectile function. |
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| المؤلفون: | Liu G; Department of Urology, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, China., Liu J; Department of Urology, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, China., Kong X; Department of Urology, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, China., Xiong WJ; Department of Urology, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, China., Jiang R; Department of Urology, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, China. |
| المصدر: | The journal of sexual medicine [J Sex Med] 2023 Aug 25; Vol. 20 (9), pp. 1153-1160. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: Netherlands NLM ID: 101230693 Publication Model: Print Cited Medium: Internet ISSN: 1743-6109 (Electronic) Linking ISSN: 17436095 NLM ISO Abbreviation: J Sex Med Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2023- : [Oxford] : Oxford University Press Original Publication: Malden, MA : Blackwell Pub. |
| مواضيع طبية MeSH: | Erectile Dysfunction*/etiology , Transient Receptor Potential Channels*/metabolism , Transient Receptor Potential Channels*/pharmacology , Transient Receptor Potential Channels*/therapeutic use, Humans ; Rats ; Male ; Animals ; TRPV Cation Channels/genetics ; TRPV Cation Channels/metabolism ; TRPV Cation Channels/pharmacology ; Rats, Sprague-Dawley ; Endothelial Cells/metabolism ; Nitrites/metabolism ; Nitrites/pharmacology ; Nitrites/therapeutic use ; Penile Erection/physiology ; Penis ; Nitric Oxide Synthase Type III/metabolism |
| مستخلص: | Background: Hypoandrogenism is a cause of erectile dysfunction (ED). Vascular smooth muscle cell contraction and relaxation are regulated by TRPV1-4 channels. However, the influence of hypoandrogenism on TRPV1-4 and its relationship with erectile function remain unclear. Aim: To reveal whether hypoandrogenism affects erectile function by influencing TRPV1-4 expression in the corpus cavernosum of rats. Methods: Male Sprague-Dawley rats (N = 36) aged 8 weeks were assigned to 6 groups at random (n = 6): sham operation, castrated, castrated + testosterone replacement, sham operation + transfection, castrated + transfection, and castrated + empty transfection. Four weeks after castration, 20 μL of lentiviral vector (1 × 108 TU/mL) carrying the TRPV4 gene was injected into the penile cavernous tissue of the transfection groups. One week after transfection, the maximum intracavernous pressure (ICPmax)/mean arterial pressure (MAP) and the content of TRPV1-4, phosphorylated eNOS (p-eNOS)/eNOS, and nitric oxide (NO) in penile cavernous tissue of each group were measured. Outcomes: Under low androgen conditions, TRPV4 expression in endothelial cells in the rat penile cavernosum was sharply reduced, resulting in a decrease in p-eNOS/eNOS and NO content, which could inhibit erectile function. Results: In rat penile cavernous tissue, TRPV1-4 was expressed in the cell membranes of endothelial cells and smooth muscle cells. The ICPmax/MAP and the content of TRPV4, p-eNOS/eNOS, and NO end product nitrite level in rat penile cavernous tissue was markedly reduced in the castrated group as compared with the sham group (P < .05). The ICPmax/MAP and the content of TRPV4, p-eNOS/eNOS, and NO end product nitrite level in rat penile cavernous tissue were markedly improved in the castrated + transfection group vs the castrated group (P < .01). Clinical Implications: Upregulation of TRPV4 expression in penile cavernosum tissue might be a viable therapeutic for ED caused by hypoandrogenism. Strengths and Limitations: The specific mechanism of TRPV4 in ED needs to be further verified by androgen receptor or TRPV4 gene knockout experiments. Conclusion: Hypoandrogenism may cause ED by reducing the expression of TRPV4 in rat penile cavernous tissue. Upregulation of TRPV4 expression in penile cavernous tissue can increase the ratio of p-eNOS/eNOS and NO levels and ameliorate the erectile function of castrated rats. (© The Author(s) 2023. Published by Oxford University Press on behalf of The International Society of Sexual Medicine. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.) |
| فهرسة مساهمة: | Keywords: TRPV; eNOS; erectile dysfunction; hypoandrogenism |
| المشرفين على المادة: | 0 (TRPV Cation Channels) 0 (Transient Receptor Potential Channels) 0 (Nitrites) EC 1.14.13.39 (Nitric Oxide Synthase Type III) |
| تواريخ الأحداث: | Date Created: 20230725 Date Completed: 20230904 Latest Revision: 20230908 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1093/jsxmed/qdad093 |
| PMID: | 37490314 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1743-6109 |
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| DOI: | 10.1093/jsxmed/qdad093 |