دورية أكاديمية
MHC Tetramers Specifically Identify High- and Low-avidity Donor-specific B Cells in Transplantation Tolerance and Rejection.
العنوان: | MHC Tetramers Specifically Identify High- and Low-avidity Donor-specific B Cells in Transplantation Tolerance and Rejection. |
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المؤلفون: | Durgam SS; Department of Surgery, The University of Chicago, Chicago, IL., Khiew SHW; Department of Surgery, The University of Chicago, Chicago, IL., Sayin I; Department of Surgery, The University of Chicago, Chicago, IL., Jain D; Department of Surgery, The University of Chicago, Chicago, IL., Yin D; Department of Surgery, The University of Chicago, Chicago, IL., Cavazzoni CB; Renal Division, Transplantation Research Center, Brigham and Women's Hospital, Harvard Medical School, Boston, MA., Sage PT; Renal Division, Transplantation Research Center, Brigham and Women's Hospital, Harvard Medical School, Boston, MA., King RG; Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL., Chong AS; Department of Surgery, The University of Chicago, Chicago, IL. |
المصدر: | Transplantation [Transplantation] 2023 Dec 01; Vol. 107 (12), pp. 2526-2532. Date of Electronic Publication: 2023 Nov 22. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 0132144 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1534-6080 (Electronic) Linking ISSN: 00411337 NLM ISO Abbreviation: Transplantation Subsets: MEDLINE |
أسماء مطبوعة: | Publication: Hagerstown, MD : Lippincott Williams & Wilkins Original Publication: Baltimore, Williams & Wilkins. |
مواضيع طبية MeSH: | Transplantation Tolerance* , Major Histocompatibility Complex*, Humans ; Mice ; Animals ; Histocompatibility Antigens Class II ; Immunoglobulin G ; Antibodies, Monoclonal ; Receptors, Antigen, B-Cell |
مستخلص: | Background: Although donor-specific antibody pre- and posttransplantation is routinely assessed, accurate quantification of memory alloreactive B cells that mediate recall antibody response remains challenging. Major histocompatibility complex (MHC) tetramers have been used to identify alloreactive B cells in mice and humans, but the specificity of this approach has not been rigorously assessed. Methods: B-cell receptors from MHC tetramer-binding single B cells were expressed as mouse recombinant immunoglobulin G1 (rIgG1) monoclonal antibodies, and the specificity was assessed with a multiplex bead assay. Relative binding avidity of rIgG1 was measured by modified dilution series technique and surface plasmon resonance. Additionally, immunoglobulin heavy chain variable regions of 50 individual B-cell receptors were sequenced to analyze the rate of somatic hypermutation. Results: The multiplex bead assay confirmed that expressed rIgG1 monoclonal antibodies were preferentially bound to bait MHC class II I-E d over control I-A d and I-A b tetramers. Furthermore, the dissociation constant 50 binding avidities of the rIgG1 ranged from 10 mM to 7 nM. The majority of tetramer-binding B cells were low avidity, and ~12.8% to 15.2% from naive and tolerant mice and 30.9% from acute rejecting mice were higher avidity (dissociation constant 50 <1 mM). Conclusions: Collectively, these studies demonstrate that donor MHC tetramers, under stringent binding conditions with decoy self-MHC tetramers, can specifically identify a broad repertoire of donor-specific B cells under conditions of rejection and tolerance. Competing Interests: The authors declare no conflicts of interest. (Copyright © 2023 Wolters Kluwer Health, Inc. All rights reserved.) |
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معلومات مُعتمدة: | P01 AI097113 United States AI NIAID NIH HHS; R01 AI142747 United States AI NIAID NIH HHS; R01 AI148705 United States AI NIAID NIH HHS |
المشرفين على المادة: | 0 (Histocompatibility Antigens Class II) 0 (Immunoglobulin G) 0 (Antibodies, Monoclonal) 0 (Receptors, Antigen, B-Cell) |
تواريخ الأحداث: | Date Created: 20230726 Date Completed: 20231128 Latest Revision: 20240218 |
رمز التحديث: | 20240218 |
مُعرف محوري في PubMed: | PMC10811295 |
DOI: | 10.1097/TP.0000000000004702 |
PMID: | 37493609 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1534-6080 |
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DOI: | 10.1097/TP.0000000000004702 |