دورية أكاديمية
أعرض في EDS

MHC Tetramers Specifically Identify High- and Low-avidity Donor-specific B Cells in Transplantation Tolerance and Rejection.

التفاصيل البيبلوغرافية
العنوان: MHC Tetramers Specifically Identify High- and Low-avidity Donor-specific B Cells in Transplantation Tolerance and Rejection.
المؤلفون: Durgam SS; Department of Surgery, The University of Chicago, Chicago, IL., Khiew SHW; Department of Surgery, The University of Chicago, Chicago, IL., Sayin I; Department of Surgery, The University of Chicago, Chicago, IL., Jain D; Department of Surgery, The University of Chicago, Chicago, IL., Yin D; Department of Surgery, The University of Chicago, Chicago, IL., Cavazzoni CB; Renal Division, Transplantation Research Center, Brigham and Women's Hospital, Harvard Medical School, Boston, MA., Sage PT; Renal Division, Transplantation Research Center, Brigham and Women's Hospital, Harvard Medical School, Boston, MA., King RG; Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL., Chong AS; Department of Surgery, The University of Chicago, Chicago, IL.
المصدر: Transplantation [Transplantation] 2023 Dec 01; Vol. 107 (12), pp. 2526-2532. Date of Electronic Publication: 2023 Nov 22.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 0132144 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1534-6080 (Electronic) Linking ISSN: 00411337 NLM ISO Abbreviation: Transplantation Subsets: MEDLINE
أسماء مطبوعة: Publication: Hagerstown, MD : Lippincott Williams & Wilkins
Original Publication: Baltimore, Williams & Wilkins.
مواضيع طبية MeSH: Transplantation Tolerance* , Major Histocompatibility Complex*, Humans ; Mice ; Animals ; Histocompatibility Antigens Class II ; Immunoglobulin G ; Antibodies, Monoclonal ; Receptors, Antigen, B-Cell
مستخلص: Background: Although donor-specific antibody pre- and posttransplantation is routinely assessed, accurate quantification of memory alloreactive B cells that mediate recall antibody response remains challenging. Major histocompatibility complex (MHC) tetramers have been used to identify alloreactive B cells in mice and humans, but the specificity of this approach has not been rigorously assessed.
Methods: B-cell receptors from MHC tetramer-binding single B cells were expressed as mouse recombinant immunoglobulin G1 (rIgG1) monoclonal antibodies, and the specificity was assessed with a multiplex bead assay. Relative binding avidity of rIgG1 was measured by modified dilution series technique and surface plasmon resonance. Additionally, immunoglobulin heavy chain variable regions of 50 individual B-cell receptors were sequenced to analyze the rate of somatic hypermutation.
Results: The multiplex bead assay confirmed that expressed rIgG1 monoclonal antibodies were preferentially bound to bait MHC class II I-E d over control I-A d and I-A b tetramers. Furthermore, the dissociation constant 50 binding avidities of the rIgG1 ranged from 10 mM to 7 nM. The majority of tetramer-binding B cells were low avidity, and ~12.8% to 15.2% from naive and tolerant mice and 30.9% from acute rejecting mice were higher avidity (dissociation constant 50 <1 mM).
Conclusions: Collectively, these studies demonstrate that donor MHC tetramers, under stringent binding conditions with decoy self-MHC tetramers, can specifically identify a broad repertoire of donor-specific B cells under conditions of rejection and tolerance.
Competing Interests: The authors declare no conflicts of interest.
(Copyright © 2023 Wolters Kluwer Health, Inc. All rights reserved.)
References: Sensors (Basel). 2015 May 05;15(5):10481-510. (PMID: 25951336)
Curr Opin Organ Transplant. 2022 Oct 1;27(5):385-391. (PMID: 35950881)
J Immunol Methods. 2010 Jun 30;358(1-2):46-55. (PMID: 20361969)
Int J Immunogenet. 2020 Jun;47(3):227-234. (PMID: 32390325)
Transplantation. 2016 Aug;100(8):1683-91. (PMID: 27362308)
Transplantation. 2012 Jul 27;94(2):172-7. (PMID: 22735711)
Nat Rev Immunol. 2021 Apr;21(4):209-220. (PMID: 33024284)
J Am Soc Nephrol. 2020 Sep;31(9):2193-2204. (PMID: 32764139)
Nat Protoc. 2013 Jun;8(6):1073-87. (PMID: 23660756)
Am J Transplant. 2012 Oct;12(10):2641-51. (PMID: 22759336)
Am J Transplant. 2011 Sep;11(9):1916-26. (PMID: 21827613)
J Immunol. 2015 Nov 1;195(9):4069-73. (PMID: 26416270)
Curr Opin Chem Biol. 2015 Feb;24:112-20. (PMID: 25461729)
J Clin Invest. 2020 Jul 1;130(7):3453-3466. (PMID: 32452834)
Nat Rev Immunol. 2015 Mar;15(3):160-71. (PMID: 25698678)
Adv Immunol. 1993;54:229-70. (PMID: 8379463)
Am J Transplant. 2019 Feb;19(2):368-380. (PMID: 30085394)
J Immunol Methods. 2009 Oct 31;350(1-2):183-93. (PMID: 19716372)
J Am Soc Nephrol. 2014 Jul;25(7):1575-85. (PMID: 24610932)
N Engl J Med. 2018 Sep 20;379(12):1150-1160. (PMID: 30231232)
Front Immunol. 2021 Sep 28;12:738123. (PMID: 34650561)
Cell Rep. 2018 Aug 21;24(8):2112-2126. (PMID: 30134172)
Lancet. 2005 Apr 30-May 6;365(9470):1570-6. (PMID: 15866311)
Immunity. 2020 Jul 14;53(1):172-186.e6. (PMID: 32610078)
Immunity. 2013 May 23;38(5):918-29. (PMID: 23684984)
Am J Transplant. 2016 Aug;16(8):2312-23. (PMID: 26928966)
Transplantation. 2019 Apr;103(4):716-723. (PMID: 30418423)
Transpl Int. 2018 Aug;31(8):900-908. (PMID: 29570868)
معلومات مُعتمدة: P01 AI097113 United States AI NIAID NIH HHS; R01 AI142747 United States AI NIAID NIH HHS; R01 AI148705 United States AI NIAID NIH HHS
المشرفين على المادة: 0 (Histocompatibility Antigens Class II)
0 (Immunoglobulin G)
0 (Antibodies, Monoclonal)
0 (Receptors, Antigen, B-Cell)
تواريخ الأحداث: Date Created: 20230726 Date Completed: 20231128 Latest Revision: 20240218
رمز التحديث: 20240218
مُعرف محوري في PubMed: PMC10811295
DOI: 10.1097/TP.0000000000004702
PMID: 37493609
قاعدة البيانات: MEDLINE
الوصف
تدمد:1534-6080
DOI:10.1097/TP.0000000000004702