التفاصيل البيبلوغرافية
| العنوان: |
Development of an Efficient, Effective, and Economical Technology for Proteome Analysis. |
| المؤلفون: |
Yu Y; University of Delaware., Martin K; University of Delaware., Le H; University of Delaware., Yang C; University of Delaware., Lu G; CDS Analytical LLC., Zhang X; CDS Analytical LLC., Grimes C; University of Delaware., Zhuang Z; University of Delaware., Asare-Okai PN; University of Delaware. |
| المصدر: |
Research square [Res Sq] 2023 Jul 17. Date of Electronic Publication: 2023 Jul 17. |
| نوع المنشور: |
Preprint |
| اللغة: |
English |
| بيانات الدورية: |
Country of Publication: United States NLM ID: 101768035 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: Res Sq Subsets: PubMed not MEDLINE |
| مستخلص: |
Proteomics experiments have typically high economic and technical barriers to broad biomedical scientists, which not only result in costly supplies and accessories for sample preparation but also the reluctance to adapt new techniques. In the present study, we present an effective and efficient, yet economical technology, which we call E3technology, for proteomics sample preparation. By immobilizing silica microparticles into a polytetrafluoroethylene (PTFE) matrix, we developed a novel medium, which could be used as a robust and reliable proteomics platform to generate LCMS-friendly samples in a rapid and low-cost fashion. Using different formats of E3technology, including E3tip, E3filter, E3cartridge, and E3plate, we explored a variety of sample types in varied complexity, quantity, volume, and size, including bacterial, fungi, mammalian cells, mouse tissue, and human body fluids. We benchmark their performance against several established approaches. Our data suggest that E3technology outperforms many of the currently available techniques in terms of proteome identification and quantitation. It is widely applicable, highly reproducible, readily scalable and automatable, and is user-friendly and stress-free to non-expert proteomics laboratories. It does not require specialized expertise and equipment, and significantly lowers the technical and economical barrier to proteomics experiments. An enhanced version, E4technology, also opens new avenues to sample preparation for low input and/or low-cell proteomics analysis. The presented technologies by our study represent a breakthrough innovation in biomedical science, and we anticipate widespread adoption by the proteomics community.
Competing Interests: Conflict of interests The authors would like to declare the following conflict of interest: G.L and X.Z are employees of CDS Analytical. Other authors declare no competing financial interest. A PCT patent application (status pending) has been filed for methods described in this study. |
| التعليقات: |
Update in: Cell Rep Methods. 2024 Jun 17;4(6):100796. doi: 10.1016/j.crmeth.2024.100796. (PMID: 38866007) |
| معلومات مُعتمدة: |
P20 GM104316 United States GM NIGMS NIH HHS |
| تواريخ الأحداث: |
Date Created: 20230728 Latest Revision: 20240624 |
| رمز التحديث: |
20240624 |
| مُعرف محوري في PubMed: |
PMC10371162 |
| DOI: |
10.21203/rs.3.rs-3165690/v1 |
| PMID: |
37502920 |
| قاعدة البيانات: |
MEDLINE |
