دورية أكاديمية
أعرض في EDS

Biocompatible Phosphorescent O 2 Sensors Based on Ir(III) Complexes for In Vivo Hypoxia Imaging.

التفاصيل البيبلوغرافية
العنوان: Biocompatible Phosphorescent O 2 Sensors Based on Ir(III) Complexes for In Vivo Hypoxia Imaging.
المؤلفون: Samandarsangari M; Institute of Chemistry, St. Petersburg State University, Universitetskaya Embankment 7-9, 199034 St. Petersburg, Russia., Kozina DO; Institute of Chemistry, St. Petersburg State University, Universitetskaya Embankment 7-9, 199034 St. Petersburg, Russia., Sokolov VV; Institute of Chemistry, St. Petersburg State University, Universitetskaya Embankment 7-9, 199034 St. Petersburg, Russia., Komarova AD; Institute of Experimental Oncology and Biomedical Technologies, Privolzhskiy Research Medical University, Minin and Pozharsky Sq. 10/1, 603005 Nizhny Novgorod, Russia.; Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, Gagarina Av., 23, 603950 Nizhny Novgorod, Russia., Shirmanova MV; Institute of Experimental Oncology and Biomedical Technologies, Privolzhskiy Research Medical University, Minin and Pozharsky Sq. 10/1, 603005 Nizhny Novgorod, Russia., Kritchenkov IS; Institute of Chemistry, St. Petersburg State University, Universitetskaya Embankment 7-9, 199034 St. Petersburg, Russia., Tunik SP; Institute of Chemistry, St. Petersburg State University, Universitetskaya Embankment 7-9, 199034 St. Petersburg, Russia.
المصدر: Biosensors [Biosensors (Basel)] 2023 Jun 26; Vol. 13 (7). Date of Electronic Publication: 2023 Jun 26.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Pub Country of Publication: Switzerland NLM ID: 101609191 Publication Model: Electronic Cited Medium: Internet ISSN: 2079-6374 (Electronic) Linking ISSN: 20796374 NLM ISO Abbreviation: Biosensors (Basel) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI Pub.
مواضيع طبية MeSH: Oxygen* , Neoplasms*, Humans ; Ligands ; Hypoxia ; Water
مستخلص: In this work, we obtained three new phosphorescent iridium complexes ( Ir1 - Ir3 ) of general stoichiometry [Ir(N^C) 2 (N^N)]Cl decorated with oligo(ethylene glycol) fragments to make them water-soluble and biocompatible, as well as to protect them from aggregation with biomolecules such as albumin. The major photophysical characteristics of these phosphorescent complexes are determined by the nature of two cyclometallating ligands (N^C) based on 2-pyridine-benzothiophene, since quantum chemical calculations revealed that the electronic transitions responsible for the excitation and emission are localized mainly at these fragments. However, the use of various diimine ligands (N^N) proved to affect the quantum yield of phosphorescence and allowed for changing the complexes' sensitivity to oxygen, due to the variations in the steric accessibility of the chromophore center for O 2 molecules. It was also found that the N^N ligands made it possible to tune the biocompatibility of the resulting compounds. The wavelengths of the Ir1 - Ir3 emission maxima fell in the range of 630-650 nm, the quantum yields reached 17% ( Ir1 ) in a deaerated solution, and sensitivity to molecular oxygen, estimated as the ratio of emission lifetime in deaerated and aerated water solutions, displayed the highest value, 8.2, for Ir1 . The obtained complexes featured low toxicity, good water solubility and the absence of a significant effect of biological environment components on the parameters of their emission. Of the studied compounds, Ir1 and Ir2 were chosen for in vitro and in vivo biological experiments to estimate oxygen concentration in cell lines and tumors. These sensors have demonstrated their effectiveness for mapping the distribution of oxygen and for monitoring hypoxia in the biological objects studied.
References: Cell Mol Life Sci. 2012 Jun;69(12):2025-39. (PMID: 22249195)
Chem Commun (Camb). 2022 Jan 4;58(3):419-422. (PMID: 34897308)
Adv Healthc Mater. 2018 Aug;7(16):e1800309. (PMID: 29968378)
Cell Rep. 2018 Jun 12;23(11):3312-3326. (PMID: 29898401)
Biomaterials. 2013 Dec;34(37):9307-17. (PMID: 24016849)
Bioconjug Chem. 2020 May 20;31(5):1327-1343. (PMID: 32223218)
Physiol Rev. 2003 Jul;83(3):933-63. (PMID: 12843412)
PLoS One. 2015 Mar 18;10(3):e0121293. (PMID: 25786221)
J Chem Theory Comput. 2012 Dec 11;8(12):4989-5007. (PMID: 26593191)
Angew Chem Int Ed Engl. 2012 Apr 23;51(17):4148-51. (PMID: 22344795)
Bioconjug Chem. 2016 Feb 17;27(2):439-45. (PMID: 26704593)
Methods Mol Biol. 2015;1254:55-71. (PMID: 25431057)
Int J Mol Sci. 2022 Sep 06;23(18):. (PMID: 36142177)
Bioconjug Chem. 2022 Nov 16;33(11):2161-2169. (PMID: 36289566)
Compr Physiol. 2013 Jul;3(3):1135-90. (PMID: 23897683)
Cell Mol Life Sci. 2017 Jan;74(1):141-151. (PMID: 27510419)
Chembiochem. 2012 May 29;13(8):1184-90. (PMID: 22532338)
Cell Metab. 2019 Mar 5;29(3):736-744.e7. (PMID: 30686745)
Biomater Sci. 2014 Jun 7;2(6):853-866. (PMID: 32481818)
Biosensors (Basel). 2023 Jun 26;13(7):. (PMID: 37504079)
Nature. 2014 Apr 10;508(7495):269-73. (PMID: 24590072)
Anal Chem. 2015 Mar 3;87(5):2710-7. (PMID: 25634116)
Nat Biomed Eng. 2018 Apr;2(4):254-264. (PMID: 30899599)
Nat Commun. 2020 Jan 29;11(1):573. (PMID: 31996677)
Angew Chem Int Ed Engl. 2014 Nov 10;53(46):12471-5. (PMID: 25044871)
Anal Chem. 2011 Nov 15;83(22):8756-65. (PMID: 21961699)
J Comput Chem. 2012 Feb 15;33(5):580-92. (PMID: 22162017)
J Neurosci Methods. 2017 Apr 01;281:1-6. (PMID: 28219725)
Am J Physiol Cell Physiol. 2015 Oct 1;309(7):C501-9. (PMID: 26246428)
Sci Rep. 2015 Dec 08;5:17838. (PMID: 26644023)
Nat Methods. 2010 Sep;7(9):755-9. (PMID: 20693997)
Cell. 2012 Feb 3;148(3):399-408. (PMID: 22304911)
Chemistry. 2019 Apr 23;25(23):6026-6037. (PMID: 30805959)
Molecules. 2022 May 14;27(10):. (PMID: 35630633)
Nanoscale. 2013 Dec 21;5(24):12633-44. (PMID: 24177494)
J Cereb Blood Flow Metab. 2022 Mar;42(3):510-525. (PMID: 32515672)
Cell Mol Life Sci. 2018 Aug;75(16):2963-2980. (PMID: 29761206)
J Comput Chem. 2008 Apr 15;29(5):839-45. (PMID: 17849392)
Nat Commun. 2015 Jan 05;6:5834. (PMID: 25556360)
ACS Nano. 2015 May 26;9(5):5275-88. (PMID: 25858428)
J Biomed Opt. 2018 Dec;23(12):1-9. (PMID: 30516039)
J Am Chem Soc. 2018 Jun 27;140(25):7827-7834. (PMID: 29874455)
Cancer Res. 2010 Jun 1;70(11):4490-8. (PMID: 20460508)
J Neurosci Methods. 2013 Jun 15;216(2):146-51. (PMID: 23624034)
Dalton Trans. 2020 May 26;49(20):6751-6763. (PMID: 32373874)
Anal Chem. 2014 Jun 17;86(12):5937-45. (PMID: 24848643)
Biomaterials. 2017 Nov;146:86-96. (PMID: 28898760)
Nature. 2020 Feb;578(7794):278-283. (PMID: 32025033)
Chem Rev. 2005 Aug;105(8):2999-3093. (PMID: 16092826)
Sci Rep. 2020 Dec 3;10(1):21053. (PMID: 33273499)
Curr Opin Chem Biol. 2016 Aug;33:39-45. (PMID: 27281510)
Molecules. 2021 May 13;26(10):. (PMID: 34068190)
Bioorg Med Chem. 2007 Jan 1;15(1):186-93. (PMID: 17079147)
Science. 2007 Oct 5;318(5847):62-4. (PMID: 17916722)
معلومات مُعتمدة: 18-73-10021 Russian Science Foundation
فهرسة مساهمة: Keywords: bioimaging; hypoxia; iridium complexes; oxygen sensing; phosphorescence; phosphorescence lifetime imaging
المشرفين على المادة: 0 (Ligands)
S88TT14065 (Oxygen)
059QF0KO0R (Water)
تواريخ الأحداث: Date Created: 20230728 Date Completed: 20230731 Latest Revision: 20230731
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10377268
DOI: 10.3390/bios13070680
PMID: 37504079
قاعدة البيانات: MEDLINE
الوصف
تدمد:2079-6374
DOI:10.3390/bios13070680