Green analytical toxicology method for determination of synthetic cathinones in oral fluid samples by microextraction by packed sorbent and liquid chromatography-tandem mass spectrometry.

التفاصيل البيبلوغرافية
العنوان:	Green analytical toxicology method for determination of synthetic cathinones in oral fluid samples by microextraction by packed sorbent and liquid chromatography-tandem mass spectrometry.
المؤلفون:	da Cunha KF; Campinas Poison Control Center, Faculty of Medical Sciences, University of Campinas, Campinas, São Paulo, 13083‑859, Brazil.; Faculty of Medical Sciences, University of Campinas, Campinas, São Paulo, 13083‑859, Brazil., Oliveira KD; Faculty of Medical Sciences, University of Campinas, Campinas, São Paulo, 13083‑859, Brazil., Costa JL; Campinas Poison Control Center, Faculty of Medical Sciences, University of Campinas, Campinas, São Paulo, 13083‑859, Brazil. jose.jlc@fcf.unicamp.br.; Faculty of Pharmaceutical Sciences, University of Campinas, Campinas, São Paulo, 13083‑859, Brazil. jose.jlc@fcf.unicamp.br.
المصدر:	Forensic toxicology [Forensic Toxicol] 2024 Jan; Vol. 42 (1), pp. 18-30. Date of Electronic Publication: 2023 Jul 28.
نوع المنشور:	Journal Article; Research Support, Non-U.S. Gov't; Comment
اللغة:	English
بيانات الدورية:	Publisher: Japanese Association of Forensic Toxicology Country of Publication: Japan NLM ID: 101315563 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1860-8965 (Print) Linking ISSN: 18608965 NLM ISO Abbreviation: Forensic Toxicol Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Tokyo : Japanese Association of Forensic Toxicology : Springer, 2006-
مواضيع طبية MeSH:	Synthetic Cathinone* , Tandem Mass Spectrometry*/methods, Chromatography, Liquid/methods ; Solid Phase Microextraction/methods ; Limit of Detection
مستخلص:	Purpose: We developed and validated a method for quantitative analysis of ten synthetic cathinones in oral fluid (OF) samples, using microextraction by packed sorbent (MEPS) for sample preparation followed by liquid chromatography‒tandem mass spectrometry (LC‒MS/MS). Method: OF samples were collected with a Quantisal™ device and 200 µL was extracted using a C18 MEPS cartridge installed on a semi-automated pipette and then analyzed using LC‒M/SMS. Results: Linearity was achieved between 0.1 and 25 ng/mL, with a limit of detection (LOD) of 0.05 ng/mL and a limit of quantification (LOQ) of 0.1 ng/mL. Imprecision (% relative standard deviation) and bias (%) were better than 11.6% and 7.5%, respectively. The method had good specificity and selectivity against 9 different blank OF samples (from different donors) and 68 pharmaceutical and drugs of abuse with concentrations varying between 400 and 10,000 ng/mL. No evidence of carryover was observed. The analytes were stable after three freeze/thaw cycles and when kept in the autosampler (10 °C) for up to 24 h. The method was successfully applied to quantify 41 authentic positive samples. Methylone (mean 0.6 ng/mL, median 0.2 ng/mL), N-ethylpentylone (mean 16.7 ng/mL, median 0.35 ng/mL), eutylone (mean 39.1 ng/mL, median 3.6 ng/mL), mephedrone (mean 0.5 ng/mL, median 0.5 ng/mL), and 4-chloroethcathinone (8.1 ng/mL) were quantified in these samples. Conclusion: MEPS was an efficient technique for Green Analytical Toxicology purposes, which required only 650 µL organic solvent and 200 µL sodium hydroxide, and the BIN cartridge had a lifespan of 100 sample extractions. (© 2023. The Author(s), under exclusive licence to Japanese Association of Forensic Toxicology.)
التعليقات:	Comment on: J Anal Toxicol. 2022 Aug 13;46(7):705-711. (PMID: 34592764)
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معلومات مُعتمدة:	2018/00432-1 Fundação de Amparo à Pesquisa do Estado de São Paulo; 2018/11849-0 Fundação de Amparo à Pesquisa do Estado de São Paulo; 2020/10809-5 Fundação de Amparo à Pesquisa do Estado de São Paulo; 2017/02147-0 Fundação de Amparo à Pesquisa do Estado de São Paulo; 001 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; 88887.516176/2020-00 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
فهرسة مساهمة:	Keywords: Green analytical toxicology (GAT); LC–MS/MS; Microextraction by packed sorbent (MEPS); Oral fluid; Synthetic cathinones
المشرفين على المادة:	0 (Synthetic Cathinone)
تواريخ الأحداث:	Date Created: 20230728 Date Completed: 20240125 Latest Revision: 20240216
رمز التحديث:	20240216
DOI:	10.1007/s11419-023-00671-z
PMID:	37505359
قاعدة البيانات:	MEDLINE

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تدمد:	1860-8965
DOI:	10.1007/s11419-023-00671-z