دورية أكاديمية
Cork sheet as an efficient biosorbent for forensic toxicology: Application to vitreous humor analysis.
| العنوان: | Cork sheet as an efficient biosorbent for forensic toxicology: Application to vitreous humor analysis. |
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| المؤلفون: | Ossanes DS; Graduate Program in Health Sciences, Federal University of Health Sciences of Porto Alegre, Porto Alegre, RS 90050-170, Brazil., Birk L; Graduate Program in Health Sciences, Federal University of Health Sciences of Porto Alegre, Porto Alegre, RS 90050-170, Brazil., Petry AUS; Graduate Program in Health Sciences, Federal University of Health Sciences of Porto Alegre, Porto Alegre, RS 90050-170, Brazil.; Division of Postmortem Inspection, Associação Hospitalar Vila Nova, Porto Alegre, RS 91750-040, Brazil.; Graduate Program in Pathology, Federal University of Health Sciences of Porto Alegre, Porto Alegre, RS 90050-170, Brazil., de Menezes FP; Division of Postmortem Inspection, Associação Hospitalar Vila Nova, Porto Alegre, RS 91750-040, Brazil., Gonzaga AP; Division of Postmortem Inspection, Associação Hospitalar Vila Nova, Porto Alegre, RS 91750-040, Brazil., Schlickmann PF; Division of Postmortem Inspection, Associação Hospitalar Vila Nova, Porto Alegre, RS 91750-040, Brazil., Eller S; Graduate Program in Health Sciences, Federal University of Health Sciences of Porto Alegre, Porto Alegre, RS 90050-170, Brazil., de Oliveira TF; Graduate Program in Health Sciences, Federal University of Health Sciences of Porto Alegre, Porto Alegre, RS 90050-170, Brazil. |
| المصدر: | Journal of analytical toxicology [J Anal Toxicol] 2023 Sep 15; Vol. 47 (7), pp. 580-587. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 7705085 Publication Model: Print Cited Medium: Internet ISSN: 1945-2403 (Electronic) Linking ISSN: 01464760 NLM ISO Abbreviation: J Anal Toxicol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2012- : Oxford : Oxford University Press Original Publication: 1977-<2011>: Niles, Ill., Preston Publications. |
| مواضيع طبية MeSH: | Vitreous Body*/chemistry , Solid Phase Microextraction*/methods, Humans ; Forensic Toxicology ; Psychotropic Drugs/analysis ; Solvents/analysis ; Benzodiazepines/analysis |
| مستخلص: | There is an increasing number of people affected worldwide by mental health disorders, such as depression and anxiety. One of the main courses of treatment, along with psychotherapy, is the use of psychoactive medications, like antidepressants and benzodiazepines. Also, the unprescribed use of these substances is a concerning public health issue. Hence, the analysis of psychotropic medications is mandatory in postmortem toxicology and various biological samples can be used for this detection, among them the vitreous humor (VH) stands out. Also, there is a demand for more sustainable and more efficient extraction methodologies according to green chemistry. An example is solid phase microextraction techniques (SPME), which use a solid sorbent and small solvent amounts. Biosorbents are substances of natural origin with sorptive properties, and they have been successfully used in SPME in environmental toxicology for water analysis, mainly. This study aimed to develop a sustainable, fast, cheap and simple SPME methodology using cork sheet strips as a biosorbent, to extract antidepressants, benzodiazepines and others from VH samples by liquid chromatography coupled to tandem mass spectrometry. The extraction was conducted in a 96-well plate using 200 µL of VH and optimization of relevant parameters for extraction was performed. For solvent optimization, two simplex-centroid experiments were planned for extraction and desorption and to evaluate time and pH, a Doehlert design experiment was performed. The analytical method for the determination and quantification of 17 substances was validated. The quantification limits were 5 ng/mL for all analytes and the calibration curves were linear between 5 and 30 ng/mL. This study was able to develop an efficient, cheap, simple and fast microextraction method for 17 analytes in VH, using strips of cork sheet for extraction and a 96-well plate as a container. Furthermore, this approach system could be automated for routine toxicology laboratories. (© The Author(s) 2023. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.) |
| معلومات مُعتمدة: | CAPES-Finance Code 001 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior |
| المشرفين على المادة: | 0 (Psychotropic Drugs) 0 (Solvents) 12794-10-4 (Benzodiazepines) |
| تواريخ الأحداث: | Date Created: 20230728 Date Completed: 20230918 Latest Revision: 20230918 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1093/jat/bkad045 |
| PMID: | 37506044 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1945-2403 |
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| DOI: | 10.1093/jat/bkad045 |