دورية أكاديمية
Tracking new insights into antifungal and anti-mycotoxigenic properties of a biofilm forming Pediococcus pentosaceus strain isolated from grain silage.
| العنوان: | Tracking new insights into antifungal and anti-mycotoxigenic properties of a biofilm forming Pediococcus pentosaceus strain isolated from grain silage. |
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| المؤلفون: | Mendonça CMN; Laboratory of Microbial Biomolecules, Department of Biochemical and Pharmaceutical Technology, University of São Paulo, 05508-000 São Paulo, Brazil; Centre for Healthy Eating and Food Innovation (HEFI), Faculty of Science and Engineering, Maastricht University - campus Venlo, Villafloraweg 1, 5928 SZ Venlo, the Netherlands., Oliveira RC; Laboratory of Microbial Biomolecules, Department of Biochemical and Pharmaceutical Technology, University of São Paulo, 05508-000 São Paulo, Brazil., Pizauro LJL; Department of Agricultural and Environmental Biotechnology, School of Agricultural and Veterinary Sciences (FCAV), UNESP, Jaboticabal, Brazil., Pereira WA; Laboratory of Microbial Biomolecules, Department of Biochemical and Pharmaceutical Technology, University of São Paulo, 05508-000 São Paulo, Brazil., Abboud K; Department of Agricultural and Environmental Biotechnology, School of Agricultural and Veterinary Sciences (FCAV), UNESP, Jaboticabal, Brazil., Almeida S; Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, 05508-000 São Paulo, Brazil., Watanabe IS; Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, 05508-000 São Paulo, Brazil., Varani AM; Department of Agricultural and Environmental Biotechnology, School of Agricultural and Veterinary Sciences (FCAV), UNESP, Jaboticabal, Brazil., Domínguez JM; Industrial Biotechnology and Environmental Engineering Group 'BiotecnIA', Chemical Engineering Department, University of Vigo (Campus Ourense), As Lagoas s/n, 32004 Ourense, Spain., Correa B; Laboratory of Mycotoxins and Toxigenic Fungi, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, 05508-900 São Paulo, Brazil., Venema K; Department of Agricultural and Environmental Biotechnology, School of Agricultural and Veterinary Sciences (FCAV), UNESP, Jaboticabal, Brazil., Azevedo POS; Laboratory of Microbial Biomolecules, Department of Biochemical and Pharmaceutical Technology, University of São Paulo, 05508-000 São Paulo, Brazil; SAZ Animal Nutrition, São Paulo, Brazil., Oliveira RPS; Laboratory of Microbial Biomolecules, Department of Biochemical and Pharmaceutical Technology, University of São Paulo, 05508-000 São Paulo, Brazil. Electronic address: rpsolive@usp.br. |
| المصدر: | International journal of food microbiology [Int J Food Microbiol] 2023 Nov 16; Vol. 405, pp. 110337. Date of Electronic Publication: 2023 Jul 23. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Publishers Country of Publication: Netherlands NLM ID: 8412849 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3460 (Electronic) Linking ISSN: 01681605 NLM ISO Abbreviation: Int J Food Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier Science Publishers, c1984- |
| مواضيع طبية MeSH: | Mycotoxins*/analysis , Aflatoxins*/analysis, Antifungal Agents/pharmacology ; Antifungal Agents/analysis ; Pediococcus pentosaceus ; Tandem Mass Spectrometry ; Silage/analysis ; Aspergillus flavus ; Edible Grain/chemistry |
| مستخلص: | The present study offers detailed insights into the antifungal and anti-mycotoxigenic potential of a biofilm forming lactic acid bacterium (Pediococcus pentosaceus) against one atoxigenic (Aspergillus flavus) and two toxigenic (Aspergillus nomius and Fusarium verticillioides) fungal strains. The antifungal effect of P. pentosaceus LBM18 strain was initially investigated through comparative analysis of fungi physiology by macroscopic visual evaluations and scanning electron microscopy examinations. The effects over fungal growth rate and asexual sporulation were additionally accessed. Furthermore, analytical evaluations of mycotoxin production were carried out by HPLC-MS/MS to provide insights on the bacterial anti-mycotoxigenic activity over fungal production of the aflatoxins B1, B2, G1 and G2 as well as fumonisins B1 and B2. Finally, reverse transcription quantitative real-time PCR (RT-qPCR) analysis was employed at the most effective bacterial inoculant concentration to evaluate, at the molecular level, the down-regulation of genes aflR, aflQ and aflD, related to the biosynthesis of aflatoxins by the strain of Aspergillus nomius. The effects over mycotoxin contamination were thought to be result of a combination of several biotic and abiotic factors, such as interaction between living beings and physical-chemical aspects of the environment, respectively. Several possible mechanisms of action were addressed along with potentially deleterious effects ascribing from P. pentosaceus misuse as biopesticide, emphasizing the importance of evaluating lactic acid bacteria safety in new applications, concentrations, and exposure scenarios. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Aflatoxins; Biofilm; Crops contamination; Fumonisin; Lactic acid bacteria; Mycotoxin |
| المشرفين على المادة: | 0 (Antifungal Agents) 0 (Mycotoxins) 0 (Aflatoxins) |
| SCR Organism: | Aspergillus nomius |
| تواريخ الأحداث: | Date Created: 20230728 Date Completed: 20230925 Latest Revision: 20230925 |
| رمز التحديث: | 20230925 |
| DOI: | 10.1016/j.ijfoodmicro.2023.110337 |
| PMID: | 37506547 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1879-3460 |
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| DOI: | 10.1016/j.ijfoodmicro.2023.110337 |