دورية أكاديمية
N 6 -Methyladenosine Directly Regulates CD40L Expression in CD4 + T Lymphocytes.
العنوان: | N 6 -Methyladenosine Directly Regulates CD40L Expression in CD4 + T Lymphocytes. |
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المؤلفون: | van Vroonhoven ECN; Center for Translational Immunology, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands., Picavet LW; Center for Translational Immunology, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands., Scholman RC; Center for Translational Immunology, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands., van den Dungen NAM; Department of Experimental Cardiology, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands., Mokry M; Department of Experimental Cardiology, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands., Evers A; Department of Medical Microbiology, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands., Lebbink RJ; Department of Medical Microbiology, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands., Calis JJA; Center for Translational Immunology, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands., Vastert SJ; Center for Translational Immunology, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands.; Department of Pediatric Rheumatology and Immunology, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands., van Loosdregt J; Center for Translational Immunology, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands. |
المصدر: | Biology [Biology (Basel)] 2023 Jul 14; Vol. 12 (7). Date of Electronic Publication: 2023 Jul 14. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101587988 Publication Model: Electronic Cited Medium: Print ISSN: 2079-7737 (Print) Linking ISSN: 20797737 NLM ISO Abbreviation: Biology (Basel) Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, 2012- |
مستخلص: | T cell activation is a highly regulated process, modulated via the expression of various immune regulatory proteins including cytokines, surface receptors and co-stimulatory proteins. N 6 -methyladenosine (m 6 A) is an RNA modification that can directly regulate RNA expression levels and it is associated with various biological processes. However, the function of m 6 A in T cell activation remains incompletely understood. We identify m 6 A as a novel regulator of the expression of the CD40 ligand (CD40L) in human CD4 + lymphocytes. Manipulation of the m 6 A 'eraser' fat mass and obesity-associated protein (FTO) and m 6 A 'writer' protein methyltransferase-like 3 (METTL3) directly affects the expression of CD40L. The m 6 A 'reader' protein YT521-B homology domain family-2 (YTHDF2) is hypothesized to be able to recognize and bind m 6 A specific sequences on the CD40L mRNA and promotes its degradation. This study demonstrates that CD40L expression in human primary CD4 + T lymphocytes is regulated via m 6 A modifications, elucidating a new regulatory mechanism in CD4 + T cell activation that could possibly be leveraged in the future to modulate T cell responses in patients with immune-related diseases. |
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فهرسة مساهمة: | Keywords: CD40 ligand; RNA methylation; T cell activation; adaptive immunity; autoimmunity; epitranscriptomics |
تواريخ الأحداث: | Date Created: 20230729 Latest Revision: 20230731 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC10376055 |
DOI: | 10.3390/biology12071004 |
PMID: | 37508433 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2079-7737 |
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DOI: | 10.3390/biology12071004 |