دورية أكاديمية
Genome-wide screening for regulators of degradation of insulin secretory granules with a fluorescent reporter.
| العنوان: | Genome-wide screening for regulators of degradation of insulin secretory granules with a fluorescent reporter. |
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| المؤلفون: | Kanai A; Department of Endocrinology & Metabolism, Juntendo University Graduate School of Medicine, Japan., Nishida Y; Department of Endocrinology & Metabolism, Juntendo University Graduate School of Medicine, Japan. Electronic address: y-nishida@juntendo.ac.jp., Iwamoto T; Department of Endocrinology & Metabolism, Juntendo University Graduate School of Medicine, Japan., Yokota M; Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan., Aoyama S; Department of Endocrinology & Metabolism, Juntendo University Graduate School of Medicine, Japan., Ueki K; Department of Endocrinology & Metabolism, Juntendo University Graduate School of Medicine, Japan., Ito M; Department of Endocrinology & Metabolism, Juntendo University Graduate School of Medicine, Japan., Uzawa H; Department of Endocrinology & Metabolism, Juntendo University Graduate School of Medicine, Japan., Iida H; Department of Endocrinology & Metabolism, Juntendo University Graduate School of Medicine, Japan., Koike M; Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan., Watada H; Department of Endocrinology & Metabolism, Juntendo University Graduate School of Medicine, Japan. |
| المصدر: | Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2023 Oct 08; Vol. 676, pp. 132-140. Date of Electronic Publication: 2023 Jul 20. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2002- >: San Diego, CA : Elsevier Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Insulin*/metabolism , Insulin-Secreting Cells*/metabolism, Insulin Secretion ; Membrane Proteins/metabolism ; Coloring Agents/metabolism ; Secretory Vesicles/metabolism ; Cytoplasmic Granules/metabolism |
| مستخلص: | Insulin is essential in controlling blood glucose levels, and its synthesis and secretion have been well investigated. In contrast, how insulin secretory granules (ISGs) are degraded in pancreatic beta cells remains largely unknown. To clarify the mechanism, we constructed a fluorescent reporter detecting ISG degradation, where EGFP and mCherry are tandemly conjugated to a cytoplasmic region of ZnT8, an ISG membrane-localized protein. Depletion of serum and amino acid stimulated lysosomal ISG degradation detected with the reporter. Next, with MIN6 cells expressing Cas9 and the reporter, we investigated the involvement of conventional Atg5/7-dependent autophagy to show that it is dispensable for the ISG degradation process. Finally, we performed genome-wide screening by enriching the cells lacking the ISG degradation and showed that pathways regulating autophagy are not identified. These results suggest that alternative degradation in lysosomes, instead of conventional autophagy, may be involved in ISG degradation. Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Yuya Nishida reports financial support was provided by Japan Society for the Promotion of Science. Hirotaka Watada reports financial support was provided by Japan Society for the Promotion of Science. Masato Koike reports financial support was provided by Japan Society for the Promotion of Science. (Copyright © 2023 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Autophagy; Crinophagy; Genome-wide screening; Insulin; Secretory granules |
| المشرفين على المادة: | 0 (Insulin) 0 (Membrane Proteins) 0 (Coloring Agents) |
| تواريخ الأحداث: | Date Created: 20230729 Date Completed: 20230904 Latest Revision: 20230904 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.bbrc.2023.07.040 |
| PMID: | 37516030 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2104 |
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| DOI: | 10.1016/j.bbrc.2023.07.040 |