دورية أكاديمية
A cross-sectional study on the prevalence and drug susceptibility pattern of methicillin-resistant Staphylococcus aureus isolated from patients in the Buea Health District, Cameroon.
| العنوان: | A cross-sectional study on the prevalence and drug susceptibility pattern of methicillin-resistant Staphylococcus aureus isolated from patients in the Buea Health District, Cameroon. |
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| المؤلفون: | Ndedy MM; Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Laboratory for Emerging Infectious Diseases, University of Buea, Buea, South West Region, Cameroon., Nyasa RB; Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon., Esemu SN; Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Laboratory for Emerging Infectious Diseases, University of Buea, Buea, South West Region, Cameroon., Kfusi JA; Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Laboratory for Emerging Infectious Diseases, University of Buea, Buea, South West Region, Cameroon., Keneh NK; Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Laboratory for Emerging Infectious Diseases, University of Buea, Buea, South West Region, Cameroon., Masalla TN; Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Laboratory for Emerging Infectious Diseases, University of Buea, Buea, South West Region, Cameroon., Ndip LM; Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Laboratory for Emerging Infectious Diseases, University of Buea, Buea, South West Region, Cameroon. |
| المصدر: | The Pan African medical journal [Pan Afr Med J] 2023 May 10; Vol. 45, pp. 28. Date of Electronic Publication: 2023 May 10 (Print Publication: 2023). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: African Field Epidemiology Network Country of Publication: Uganda NLM ID: 101517926 Publication Model: eCollection Cited Medium: Internet ISSN: 1937-8688 (Electronic) NLM ISO Abbreviation: Pan Afr Med J Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Kampala, Uganda : African Field Epidemiology Network |
| مواضيع طبية MeSH: | Methicillin-Resistant Staphylococcus aureus*/isolation & purification , Staphylococcal Infections*/drug therapy , Staphylococcal Infections*/epidemiology, Cameroon/epidemiology ; Cross-Sectional Studies ; Prevalence ; Anti-Bacterial Agents/therapeutic use ; Humans ; Male ; Female ; Young Adult ; Adult ; Middle Aged ; Gene Amplification |
| مستخلص: | Introduction: Staphylococcus aureus, which is part of the normal flora accounts for most acute and chronic infections in humans, and treatment options are greatly limited, when infection is caused by methicillin-resistant Staphylococcus aureus (MRSA). This study was to determine the prevalence and antimicrobial susceptibility pattern of MRSA from clinical samples obtained randomly from patients in Buea Health District. Methods: a total of 264 wounds, nasopharynx, and urine samples were collected from patients from different hospitals in Buea and transported to the laboratory in the University of Buea, for analysis. Samples were inoculated on mannitol salt agar for S. aureus isolation, characterized morphologically by gram staining and biochemically by catalase, coagulase, and hemolysis tests. Diagnosis of S. aureus was confirmed by molecular identification of the nuc gene. MRSA was identified from S. aureus by oxacillin screening and confirmed by molecular identification of the mecA gene. The data were analyzed using SPSS version 17.0. Results: S. aureus was isolated from 70 (26.52%) and all were confirmed molecularly by nuc gene amplification. MRSA by oxacillin screening was 36 (13.64%) while MRSA detected by mecA gene amplification was 34 (12.88%). Antimicrobial susceptibility testing revealed 100% resistance to ampicillin, 88.24% to cefixime and 70.59% to ceftriaxone while low resistance was observed to meropenem (11.76%), doxycycline (14.71%), and vancomycin (17.67%). Conclusion: MRSA isolated from Buea Health District are resistant to ampicillin, cefixime, and ceftriaxone. The antimicrobials (meropenem, doxycycline, and vancomycin) should be used to treat MRSA infections in Buea Health District. Competing Interests: The authors declare no competing interests. (Copyright: Morgan Mokeo Ndedy et al.) |
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| فهرسة مساهمة: | Keywords: Buea Health District; Methicillin-resistant Staphylococcus aureus; nasopharynx; urine; wounds |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (mecA protein, Staphylococcus aureus) |
| تواريخ الأحداث: | Date Created: 20230731 Date Completed: 20231018 Latest Revision: 20231018 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC10386538 |
| DOI: | 10.11604/pamj.2023.45.28.36860 |
| PMID: | 37521764 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1937-8688 |
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| DOI: | 10.11604/pamj.2023.45.28.36860 |