دورية أكاديمية
Up-regulation of Trim28 in pregnancy-induced hypertension is involved in the injury of human umbilical vein endothelial cells through the p38 signaling pathway.
| العنوان: | Up-regulation of Trim28 in pregnancy-induced hypertension is involved in the injury of human umbilical vein endothelial cells through the p38 signaling pathway. |
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| المؤلفون: | Chen M; Department of Obstetrics and Gynecology, The Affiliated Taian City Central Hospital of Qingdao University, Taian, Shandong Province, PR China., Shi P; Department of Obstetrics and Gynecology, The Affiliated Taian City Central Hospital of Qingdao University, Taian, Shandong Province, PR China., Wang P; Department of Obstetrics and Gynecology, The Affiliated Taian City Central Hospital of Qingdao University, Taian, Shandong Province, PR China., Zhang T; Department of Obstetrics and Gynecology, The Affiliated Taian City Central Hospital of Qingdao University, Taian, Shandong Province, PR China., Zhao J; Department of Obstetrics and Gynecology, The Affiliated Taian City Central Hospital of Qingdao University, Taian, Shandong Province, PR China., Zhao L; Department of Obstetrics and Gynecology, The Affiliated Taian City Central Hospital of Qingdao University, Taian, Shandong Province, PR China. tazxyycch@126.com. |
| المصدر: | Histology and histopathology [Histol Histopathol] 2024 May; Vol. 39 (5), pp. 603-610. Date of Electronic Publication: 2023 Jul 21. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Histology and Histopathology Country of Publication: Spain NLM ID: 8609357 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1699-5848 (Electronic) Linking ISSN: 02133911 NLM ISO Abbreviation: Histol Histopathol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Murcia : Histology and Histopathology Original Publication: Murcia, Spain : Gutenberg, 1986- |
| مواضيع طبية MeSH: | Apoptosis* , Cell Movement* , Cell Proliferation* , Human Umbilical Vein Endothelial Cells*/metabolism , Hypertension, Pregnancy-Induced*/metabolism , Hypertension, Pregnancy-Induced*/pathology , p38 Mitogen-Activated Protein Kinases*/metabolism , Tripartite Motif-Containing Protein 28*/metabolism , Up-Regulation*, Adult ; Female ; Humans ; Pregnancy ; MAP Kinase Signaling System ; Placenta/metabolism ; Signal Transduction ; Mitogen-Activated Protein Kinase 14 |
| مستخلص: | Aims: The present study is to analyze the regulation and potential molecular mechanism of Trim28 on vascular endothelial injury induced by pregnancy-induced hypertension (PIH). Methods: Trim28 mRNA in placental tissues and peripheral blood from PIH patients were determined by quantitative real-time polymerase chain reaction. The serum from PIH was used to stimulate human umbilical vein endothelial cells (HUVECs). After silencing Trim28 in HUVECs, we used CCK-8 assay, Transwell assay and flow cytometry to investigate proliferation, migration and apoptosis. Western blotting was used to measure Trim28 protein level and p38 phosphorylation level. After addition of p38 inhibitor, the proliferation, migration and apoptosis of HUVECs with silenced Trim28 were studied again. Results: Trim28 expression in placental tissues and peripheral blood from PIH patients is elevated, and serum from these patients can up-regulate the expression of Trim28 in HUVECs in vitro. Trim28 silencing significantly inhibits the proliferation and migration of HUVECs by affecting the cell cycle. Down-regulation of Trim28 expression promotes the apoptosis of HUVECs. Trim28 regulates the biological function of HUVECs by affecting the activity of the p38 signaling pathway. Conclusions: The present study demonstrates that Trim28 is up-regulated in peripheral blood of patients with PIH and participates in HUVECs injury through the p38 signaling pathway. (©The Author(s) 2024. Open Access. This article is licensed under a Creative Commons CC-BY International License.) |
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| المشرفين على المادة: | EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases) EC 2.3.2.27 (TRIM28 protein, human) EC 2.3.2.27 (Tripartite Motif-Containing Protein 28) EC 2.7.11.24 (Mitogen-Activated Protein Kinase 14) |
| تواريخ الأحداث: | Date Created: 20230731 Date Completed: 20240424 Latest Revision: 20240506 |
| رمز التحديث: | 20240507 |
| DOI: | 10.14670/HH-18-651 |
| PMID: | 37522419 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1699-5848 |
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| DOI: | 10.14670/HH-18-651 |