دورية أكاديمية
Proteomics analyses of human plasma reveal triosephosphate isomerase as a potential blood marker of methotrexate resistance in rheumatoid arthritis.
| العنوان: | Proteomics analyses of human plasma reveal triosephosphate isomerase as a potential blood marker of methotrexate resistance in rheumatoid arthritis. |
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| المؤلفون: | Escal J; Université Jean Monnet Saint-Étienne, CHU Saint-Etienne, Laboratoire de Pharmacologie et Toxicologie, INSERM, SAINBIOSE U1059, Saint-Etienne, France., Neel T; CHU Saint-Étienne, Service de Rhumatologie, Saint-Etienne, France., Hodin S; Université Jean Monnet Saint-Étienne, INSERM, SAINBIOSE U1059, Saint-Etienne, France., Boussoualim K; CHU Saint-Étienne, Service de Rhumatologie, Saint-Etienne, France., Amouzougan A; CHU Saint-Étienne, Service de Rhumatologie, Saint-Etienne, France., Coassy A; CHU Saint-Étienne, Service de Rhumatologie, Saint-Etienne, France., Locrelle H; CHU Saint-Étienne, Service de Rhumatologie, Saint-Etienne, France., Thomas T; Université Jean Monnet Saint-Étienne, CHU Saint-Étienne, Service de Rhumatologie, Mines Saint-Etienne, INSERM, SAINBIOSE U1059, Saint-Etienne, France., Delavenne X; Université Jean Monnet Saint-Étienne, CHU Saint-Etienne, Laboratoire de Pharmacologie et Toxicologie, INSERM, SAINBIOSE U1059, Saint-Etienne, France., Marotte H; Université Jean Monnet Saint-Étienne, CHU Saint-Étienne, Service de Rhumatologie, Mines Saint-Etienne, INSERM, SAINBIOSE U1059, Saint-Etienne, France. |
| المصدر: | Rheumatology (Oxford, England) [Rheumatology (Oxford)] 2024 May 02; Vol. 63 (5), pp. 1368-1376. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 100883501 Publication Model: Print Cited Medium: Internet ISSN: 1462-0332 (Electronic) Linking ISSN: 14620324 NLM ISO Abbreviation: Rheumatology (Oxford) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Oxford, UK : Avenel, N.J. : Oxford University Press ; Distributed by Mercury International, c1999- |
| مواضيع طبية MeSH: | Triose-Phosphate Isomerase*/blood , Arthritis, Rheumatoid*/drug therapy , Arthritis, Rheumatoid*/blood , Methotrexate*/therapeutic use , Proteomics* , Biomarkers*/blood , Drug Resistance* , Antirheumatic Agents*/therapeutic use, Humans ; Female ; Male ; Middle Aged ; Adult ; Aged ; Glucose-6-Phosphate Isomerase/blood ; Blood Proteins/metabolism |
| مستخلص: | Objective: The objective of this study was to assess differentially expressed blood proteins between patients with active RA and patients in remission after MTX treatment, with the aim of identifying a biomarker of MTX resistance (MTXR). Methods: Two populations of RA patients treated with a stable dose of s.c. MTX for at least 3 months were constituted according to the DAS28: remission (DAS28 < 2.6; n = 24) and active disease (DAS28 > 3.2; n = 32). The two groups of RA patients were homogeneous regarding their epidemiological characteristics, except for the duration of treatment, which was longer in the remission group. After collection of a blood sample, plasma protein digestion was performed, followed by untargeted proteomics analysis. Then, a targeted analysis was performed to confirm the results of the untargeted approach. Results: Untargeted proteomics analysis revealed eight plasma proteins that were differentially expressed between the two groups of patients. Among them, triosephosphate isomerase (TPI-1) and glucose-6-phosphate isomerase (GPI), which are main actors in glycolysis, were found down-regulated in the active group. This result was confirmed for TPI-1 in the targeted proteomics analysis. Conclusion: A first step was achieved in the search for biomarkers of MTXR, with the identification of two actors in glycolysis (TPI-1 and GPI). The next step will be to confirm these results in a larger cohort, including samples from treatment-naive patients, to assess the predictive potential of these protein markers. (© The Author(s) 2023. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved. For permissions, please email: journals.permissions@oup.com.) |
| فهرسة مساهمة: | Keywords: RA; biomarker; methotrexate; proteomics |
| المشرفين على المادة: | EC 5.3.1.1 (Triose-Phosphate Isomerase) YL5FZ2Y5U1 (Methotrexate) 0 (Biomarkers) 0 (Antirheumatic Agents) EC 5.3.1.9 (Glucose-6-Phosphate Isomerase) 0 (Blood Proteins) |
| تواريخ الأحداث: | Date Created: 20230801 Date Completed: 20240502 Latest Revision: 20240521 |
| رمز التحديث: | 20240521 |
| DOI: | 10.1093/rheumatology/kead390 |
| PMID: | 37527020 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1462-0332 |
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| DOI: | 10.1093/rheumatology/kead390 |