دورية أكاديمية
In silico and in vitro safety assessment of a fungal biomass from Rhizomucor pusillus for use as a novel food ingredient.
| العنوان: | In silico and in vitro safety assessment of a fungal biomass from Rhizomucor pusillus for use as a novel food ingredient. |
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| المؤلفون: | Scaife K; Intertek Health Sciences Inc., 2233 Argentia Road, Suite 21, Mississauga, ON, L5N 2X7, Canada. Electronic address: kevin.scaife@intertek.com., Vo TD; Intertek Health Sciences Inc., 2233 Argentia Road, Suite 21, Mississauga, ON, L5N 2X7, Canada., Dommels Y; The Protein Brewery B.V., Goeseelsstraat 10, 4817, MV, Breda, the Netherlands., Leune E; The Protein Brewery B.V., Goeseelsstraat 10, 4817, MV, Breda, the Netherlands., Albermann K; Labvantage - Biomax GmbH, Robert-Koch-Str. 2, 82152, Planegg, Germany., Pařenicová L; The Protein Brewery B.V., Goeseelsstraat 10, 4817, MV, Breda, the Netherlands; BioXact, Böttgerwater 44, 2497, ZJ, Den Haag, Netherlands. |
| المصدر: | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2023 Sep; Vol. 179, pp. 113972. Date of Electronic Publication: 2023 Jul 31. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 8207483 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6351 (Electronic) Linking ISSN: 02786915 NLM ISO Abbreviation: Food Chem Toxicol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Exeter : Elsevier Science Ltd Original Publication: Oxford ; New York : Pergamon Press, c1982- |
| مواضيع طبية MeSH: | Food Ingredients*, Humans ; Biomass ; Rhizomucor/genetics |
| مستخلص: | To address the growing world population and reduce the impact of environmental changes on the global food supply, ingredients are being produced using microorganisms to yield sustainable and innovative products. Food ingredients manufactured using modern biotechnology must be produced by non-toxigenic and nonpathogenic production organisms that do not harbor antimicrobial resistance (AMR). Several fungal species represent attractive targets as sources of alternative food products. One such product is a fungal biomass obtained from the fermentation of Rhizomucor pusillus strain CBS 143028. The whole genome sequence of this strain was annotated and subjected to sequence homology searches and in silico phenotype prediction tools to identify genetic elements encoding for protein toxins active via oral consumption, virulence factors associated with pathogenicity, and determinants of AMR. The in silico investigation revealed no genetic elements sharing significant sequence homology with putative virulence factors, protein toxins, or AMR determinants, including the absence of mucoricin, an essential toxin in the pathogenesis of mucormycosis. These in silico findings were corroborated in vitro based on the absence of clinically relevant mycotoxin or antibacterial secondary metabolites. Consequently, it is unlikely that R. pusillis strain CBS 143028 would pose a safety concern for use in food for human consumption. Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Kevin Scaife reports financial support was provided by The Protein Brewery B.V. Trung Vo reports financial support was provided by The Protein Brewery B.V. Yvonne Dommels reports financial support was provided by The Protein Brewery B.V. Elisa Leune reports financial support was provided by The Protein Brewery B.V. Kaj Albermann reports financial support was provided by The Protein Brewery B.V. Lucie Parenicova reports financial support was provided by The Protein Brewery B.V. Kevin Scaife reports a relationship with The Protein Brewery B.V. that includes: consulting or advisory. Trung Vo reports a relationship with The Protein Brewery B.V. that includes: consulting or advisory. Yvonne Dommels reports a relationship with The Protein Brewery B.V. that includes: employment. Elisa Leune reports a relationship with The Protein Brewery B.V. that includes: employment. Kaj Albermann reports a relationship with The Protein Brewery B.V. that includes: non-financial support. Lucie Parenicova reports a relationship with The Protein Brewery B.V. that includes: employment. (Copyright © 2023 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Bioinformatics; Food safety; Fungal biomass; Meat alternative; Rhizomucor pusillus; Sustainability |
| المشرفين على المادة: | 0 (Food Ingredients) |
| SCR Organism: | Rhizomucor pusillus |
| تواريخ الأحداث: | Date Created: 20230802 Date Completed: 20230907 Latest Revision: 20230907 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.fct.2023.113972 |
| PMID: | 37532172 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-6351 |
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| DOI: | 10.1016/j.fct.2023.113972 |