دورية أكاديمية
In situ formation of fluorescence species for the detection of alkaline phosphatase and organophosphorus pesticide via the ascorbate oxidase mimetic activity of AgPd bimetallic nanoflowers.
| العنوان: | In situ formation of fluorescence species for the detection of alkaline phosphatase and organophosphorus pesticide via the ascorbate oxidase mimetic activity of AgPd bimetallic nanoflowers. |
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| المؤلفون: | Luo L; Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China., Liu J; Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China., Liu Y; Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China., Chen H; Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China., Zhang Y; Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China., Liu M; Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China. Electronic address: liuml@hunnu.edu.cn., Yao S; Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China. |
| المصدر: | Food chemistry [Food Chem] 2024 Jan 01; Vol. 430, pp. 137062. Date of Electronic Publication: 2023 Jul 29. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 7702639 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7072 (Electronic) Linking ISSN: 03088146 NLM ISO Abbreviation: Food Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Barking : Elsevier Applied Science Publishers Original Publication: Barking, Eng., Applied Science Publishers. |
| مواضيع طبية MeSH: | Alkaline Phosphatase* , Pesticides*, Ascorbate Oxidase ; Organophosphorus Compounds ; Fluorescent Dyes ; Limit of Detection |
| مستخلص: | Sensitive detection strategies for enzymes and their inhibitors not only have critical significance in clinical diagnosis but can be extended to food and environment analysis. Here, bimetallic AgPd alloy nanoflowers were synthesized using carbon dots as the reductant via a two-step reduction method. The AgPd bimetallic nanoflowers exhibit ascorbate oxidase mimetic activity, which can rapidly catalyze the oxidization of ascorbic acid to dehydroascorbic acid (DHA). The DHA can then react with o-phenylenediamine (OPD) to produce 3-(1, 2-dihydroxyethyl)furo[3,4-b] quinoxalin-1(3H)-one (DFQ), accompanying with the characteristic fluorescence peak at 440 nm for DFQ. Alkaline phosphatase catalyzes l-ascorbyl-2-phosphate to generate AA, while organic phosphorus pesticides (OPPs) such as chlorpyrifos can inhibit the activity of alkaline phosphatase. By utilizing the enzyme-nanozyme tandem catalytic reaction, the fluorescence of the system varies in the presence of different concentrations of ALP or OPPs, which can be used to detect ALP in serum and OPPs in vegetables and fruits. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: AgPd/C nanoflowers; Alkaline phosphatase (ALP); Ascorbate oxidase (AAO); C-dots; Organophosphorus pesticides (OPPs) |
| المشرفين على المادة: | EC 3.1.3.1 (Alkaline Phosphatase) EC 1.10.3.3 (Ascorbate Oxidase) 0 (Organophosphorus Compounds) 0 (Pesticides) 0 (Fluorescent Dyes) |
| تواريخ الأحداث: | Date Created: 20230805 Date Completed: 20230907 Latest Revision: 20230907 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.foodchem.2023.137062 |
| PMID: | 37542966 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-7072 |
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| DOI: | 10.1016/j.foodchem.2023.137062 |