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Calculation of ATP production rates using the Seahorse XF Analyzer.

التفاصيل البيبلوغرافية
العنوان: Calculation of ATP production rates using the Seahorse XF Analyzer.
المؤلفون: Desousa BR; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, USA., Kim KK; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, USA., Jones AE; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, USA., Ball AB; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, USA., Hsieh WY; Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA, USA., Swain P; Agilent Technologies, Santa Clara, CA, USA., Morrow DH; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, USA., Brownstein AJ; Department of Medicine, University of California, Los Angeles, Los Angeles, CA, USA., Ferrick DA; Agilent Technologies, Santa Clara, CA, USA., Shirihai OS; Department of Medicine, University of California, Los Angeles, Los Angeles, CA, USA., Neilson A; Agilent Technologies, Santa Clara, CA, USA., Nathanson DA; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, USA., Rogers GW; Agilent Technologies, Santa Clara, CA, USA., Dranka BP; Agilent Technologies, Santa Clara, CA, USA., Murphy AN; Cytokinetics Inc., South San Francisco, CA, USA., Affourtit C; School of Biomedical Sciences, University of Plymouth, Plymouth, UK., Bensinger SJ; Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA, USA., Stiles L; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, USA.; Department of Medicine, University of California, Los Angeles, Los Angeles, CA, USA., Romero N; Agilent Technologies, Santa Clara, CA, USA., Divakaruni AS; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, USA.
المصدر: EMBO reports [EMBO Rep] 2023 Oct 09; Vol. 24 (10), pp. e56380. Date of Electronic Publication: 2023 Aug 07.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 100963049 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-3178 (Electronic) Linking ISSN: 1469221X NLM ISO Abbreviation: EMBO Rep Subsets: MEDLINE
أسماء مطبوعة: Publication: 2024- : [London] : Nature Publishing Group
Original Publication: Oxford, UK : Published for EMBO by Oxford University Press, 2000-
مواضيع طبية MeSH: Smegmamorpha*/metabolism, Animals ; Mitochondria/metabolism ; Energy Metabolism ; Glycolysis ; Oxidative Phosphorylation ; Adenosine Triphosphate/metabolism ; Mammals/metabolism
مستخلص: Oxidative phosphorylation and glycolysis are the dominant ATP-generating pathways in mammalian metabolism. The balance between these two pathways is often shifted to execute cell-specific functions in response to stimuli that promote activation, proliferation, or differentiation. However, measurement of these metabolic switches has remained mostly qualitative, making it difficult to discriminate between healthy, physiological changes in energy transduction or compensatory responses due to metabolic dysfunction. We therefore present a broadly applicable method to calculate ATP production rates from oxidative phosphorylation and glycolysis using Seahorse XF Analyzer data and empirical conversion factors. We quantify the bioenergetic changes observed during macrophage polarization as well as cancer cell adaptation to in vitro culture conditions. Additionally, we detect substantive changes in ATP utilization upon neuronal depolarization and T cell receptor activation that are not evident from steady-state ATP measurements. This method generates a single readout that allows the direct comparison of ATP produced from oxidative phosphorylation and glycolysis in live cells. Additionally, the manuscript provides a framework for tailoring the calculations to specific cell systems or experimental conditions.
(© 2023 The Authors. Published under the terms of the CC BY 4.0 license.)
References: Cell Metab. 2016 Jan 12;23(1):27-47. (PMID: 26771115)
Cell Stem Cell. 2016 Oct 6;19(4):476-490. (PMID: 27618217)
Anal Chem. 2009 Aug 15;81(16):6868-78. (PMID: 19555051)
Elife. 2020 Aug 14;9:. (PMID: 32795388)
EMBO Rep. 2023 Oct 9;24(10):e56380. (PMID: 37548091)
Nature. 2011 Apr 28;472(7344):476-80. (PMID: 21525932)
Immunity. 2013 Feb 21;38(2):225-36. (PMID: 23415911)
Cell Rep. 2020 Feb 11;30(6):1898-1909.e4. (PMID: 32049019)
Stem Cells. 2008 Apr;26(4):960-8. (PMID: 18218821)
Biochem J. 2011 Apr 15;435(2):297-312. (PMID: 21726199)
Arterioscler Thromb Vasc Biol. 2004 Jan;24(1):23-8. (PMID: 14525794)
Mol Cell. 2014 Jul 17;55(2):253-63. (PMID: 24882210)
Biochim Biophys Acta. 2015 Feb;1847(2):171-181. (PMID: 25449966)
Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):1127-31. (PMID: 8430084)
PLoS One. 2014 Jun 16;9(6):e99413. (PMID: 24932585)
J Biol Chem. 2013 Jan 11;288(2):915-26. (PMID: 23152496)
Annu Rev Cell Dev Biol. 2011;27:441-64. (PMID: 21985671)
J Neurochem. 2009 May;109(4):1179-91. (PMID: 19519782)
Cell Metab. 2012 Jun 6;15(6):827-37. (PMID: 22682223)
Methods Enzymol. 2009;457:405-24. (PMID: 19426881)
Mol Cell Biol. 2003 Feb;23(3):1085-94. (PMID: 12529412)
J Biol Chem. 2005 Aug 12;280(32):28894-902. (PMID: 15932874)
Curr Opin Neurobiol. 2020 Aug;63:111-121. (PMID: 32559637)
Cell. 2018 Sep 20;175(1):117-132.e21. (PMID: 30197082)
NMR Biomed. 2012 Nov;25(11):1234-44. (PMID: 22419606)
Diabetes. 2001 Feb;50(2):361-6. (PMID: 11272148)
STAR Protoc. 2020 Dec 19;2(1):100235. (PMID: 33364623)
Cell. 2014 Dec 18;159(7):1603-14. (PMID: 25525878)
Cell. 2017 May 4;169(4):570-586. (PMID: 28475890)
Trends Immunol. 2017 Jun;38(6):395-406. (PMID: 28396078)
Cell Metab. 2016 Jul 12;24(1):158-66. (PMID: 27374498)
Methods Enzymol. 2014;547:309-54. (PMID: 25416364)
J Mol Cell Cardiol. 2009 Jul;47(1):96-103. (PMID: 19269295)
Nat Metab. 2020 Feb;2(2):127-129. (PMID: 32694689)
Nature. 2019 Nov;575(7782):380-384. (PMID: 31666695)
J Nucl Med. 2008 Jun;49 Suppl 2:24S-42S. (PMID: 18523064)
Proc Natl Acad Sci U S A. 2010 Sep 28;107(39):16823-7. (PMID: 20847295)
Biochem Soc Trans. 2005 Nov;33(Pt 5):897-904. (PMID: 16246006)
Methods Mol Biol. 2011;793:63-75. (PMID: 21913093)
J Cell Biol. 2017 Apr 3;216(4):1091-1105. (PMID: 28254829)
Nat Immunol. 2013 Oct;14(10):1064-72. (PMID: 23955661)
J Biol Chem. 2017 Apr 28;292(17):7189-7207. (PMID: 28270511)
Essays Biochem. 2010;47:1-23. (PMID: 20533897)
Cell Rep. 2016 Feb 23;14(7):1590-1601. (PMID: 26876179)
Cancer Metab. 2014 Jun 05;2:7. (PMID: 24982758)
Methods Enzymol. 2014;547:355-71. (PMID: 25416365)
Am J Physiol. 1997 Apr;272(4 Pt 1):C1345-51. (PMID: 9142861)
Methods Enzymol. 2014;542:125-49. (PMID: 24862264)
Cell Rep. 2016 Oct 11;17(3):684-696. (PMID: 27732846)
Methods Mol Biol. 2019;1897:425-431. (PMID: 30539462)
Annu Rev Immunol. 2013;31:259-83. (PMID: 23298210)
Cell Metab. 2017 Jan 10;25(1):27-42. (PMID: 27641100)
Nat Metab. 2022 Aug;4(8):978-994. (PMID: 35971004)
Science. 1992 Sep 25;257(5078):1906-12. (PMID: 1329199)
J Biol Chem. 2022 Sep;298(9):102286. (PMID: 35868562)
Mol Cell Endocrinol. 2010 Apr 29;318(1-2):24-33. (PMID: 19720110)
Cell. 2017 Oct 5;171(2):358-371.e9. (PMID: 28985563)
Cell. 2016 Oct 6;167(2):457-470.e13. (PMID: 27667687)
J Biomol Screen. 2003 Oct;8(5):566-70. (PMID: 14567784)
معلومات مُعتمدة: T32 GM136547 United States GM NIGMS NIH HHS; R01 HL157710 United States HL NHLBI NIH HHS; T32 CA009056 United States CA NCI NIH HHS; R35 GM138003 United States GM NIGMS NIH HHS; P30 DK063491 United States DK NIDDK NIH HHS; P50 CA092131 United States CA NCI NIH HHS; P01 HL146358 United States HL NHLBI NIH HHS; T32 GM136614 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: ATP; ECAR; Seahorse XF Analyzer; glycolysis; oxidative phosphorylation
المشرفين على المادة: 8L70Q75FXE (Adenosine Triphosphate)
تواريخ الأحداث: Date Created: 20230807 Date Completed: 20231010 Latest Revision: 20240210
رمز التحديث: 20240210
مُعرف محوري في PubMed: PMC10561364
DOI: 10.15252/embr.202256380
PMID: 37548091
قاعدة البيانات: MEDLINE
الوصف
تدمد:1469-3178
DOI:10.15252/embr.202256380