دورية أكاديمية
أعرض في EDS

CYLD stimulates macrophage phagocytosis of leukemic cells through STAT1 signalling in acute myeloid leukemia.

التفاصيل البيبلوغرافية
العنوان: CYLD stimulates macrophage phagocytosis of leukemic cells through STAT1 signalling in acute myeloid leukemia.
المؤلفون: Huyen NT; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Cau Giay, Ha Noi, Vietnam.; Faculty of Biotechnology, Vietnam National University of Agriculture, Gia Lam, Hanoi, Vietnam., Ngoc NT; University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, Cau Giay, Ha Noi, Vietnam., Giang NH; Institute of Genome Research, Vietnam Academy of Science and Technology, Cau Giay, Hanoi, Vietnam., Trang DT; Institute of Genome Research, Vietnam Academy of Science and Technology, Cau Giay, Hanoi, Vietnam., Hanh HH; Institute of Genome Research, Vietnam Academy of Science and Technology, Cau Giay, Hanoi, Vietnam., Binh VD; National Institute of Hematology and Blood Transfusion, Pham Van Bach, Ha Noi, Vietnam., Giang NV; Faculty of Biotechnology, Vietnam National University of Agriculture, Gia Lam, Hanoi, Vietnam., Canh NX; Faculty of Biotechnology, Vietnam National University of Agriculture, Gia Lam, Hanoi, Vietnam., Xuan NT; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Cau Giay, Ha Noi, Vietnam.; Institute of Genome Research, Vietnam Academy of Science and Technology, Cau Giay, Hanoi, Vietnam.
المصدر: PloS one [PLoS One] 2023 Aug 07; Vol. 18 (8), pp. e0283586. Date of Electronic Publication: 2023 Aug 07 (Print Publication: 2023).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Leukemia, Myeloid, Acute*/genetics, Humans ; Macrophages/metabolism ; Cytokines/metabolism ; Phagocytosis ; RNA, Small Interfering ; Deubiquitinating Enzyme CYLD/metabolism ; STAT1 Transcription Factor/genetics ; STAT1 Transcription Factor/metabolism
مستخلص: Acute myeloid leukemia (AML) is the most aggressive hematopoietic malignancy characterized by uncontrolled proliferation of myeloid progenitor cells within the bone marrow. Tumor suppressor cylindromatosis (CYLD) is a deubiquitinating enzyme, which suppresses inflammatory response in macrophages. Macrophages have a central role in the defense against foreign substances and circulating cancer cells by their professional phagocytic capacity. Little is known about contributions of CYLD to changes in biological properties of human macrophages and its involvement in AML. The present study, therefore, explored whether macrophage functions in healthy individuals and AML patients are influenced by CYLD. To this end, ninety-two newly diagnosed AML patients and 80 healthy controls were recruited. The mRNA expression levels of inflammation-related genes were evaluated by real-time PCR, cell maturation, phagocytosis and apoptosis assays by flow cytometry and secretion of inflammatory cytokines by ELISA. As a result, AML patients with the low CYLD expression were significantly higher in M4/M5 than other subtypes according to the FAB type. The low CYLD expression was also closely associated with older patients and enhanced level of LDH in AML. Moreover, treatment of normal macrophages with CYLD siRNA enhanced activation of STAT-1, leading to increases in expressions of maturation markers and IL-6 production as well as suppression in cell apoptosis and phagocytosis, while macrophage phagocytosis from AML M4/M5b was higher than that from healthy controls upon CYLD siRNA transfection through STAT1 signalling. In conclusion, the inhibitory effects of CYLD on macrophage functions are expected to affect the immune response in AML.
Competing Interests: The authors of this paper declare that they have no financial/commercial conflicts of interests.
(Copyright: © 2023 Huyen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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المشرفين على المادة: 0 (Cytokines)
0 (RNA, Small Interfering)
EC 3.4.19.12 (Deubiquitinating Enzyme CYLD)
0 (STAT1 protein, human)
0 (STAT1 Transcription Factor)
EC 3.4.19.12 (CYLD protein, human)
تواريخ الأحداث: Date Created: 20230807 Date Completed: 20230809 Latest Revision: 20230809
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10406188
DOI: 10.1371/journal.pone.0283586
PMID: 37549179
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0283586