دورية أكاديمية
أعرض في EDS

PDIA3 modulates genomic response to 1,25-dihydroxyvitamin D 3 in squamous cell carcinoma of the skin.

التفاصيل البيبلوغرافية
العنوان: PDIA3 modulates genomic response to 1,25-dihydroxyvitamin D 3 in squamous cell carcinoma of the skin.
المؤلفون: Nowak JI; Department of Histology, Medical University of Gdansk, 1a Dębinki, 80-211 Gdansk, Poland. Electronic address: j.chorzepa@gumed.edu.pl., Olszewska AM; Department of Histology, Medical University of Gdansk, 1a Dębinki, 80-211 Gdansk, Poland. Electronic address: anna.olszewska@gumed.edu.pl., Piotrowska A; Department of Histology, Medical University of Gdansk, 1a Dębinki, 80-211 Gdansk, Poland. Electronic address: annapiotrowska@gumed.edu.pl., Myszczyński K; Centre of Biostatistics and Bioinformatics Analysis Medical University of Gdansk, 1a Debinki, 80-211 Gdansk, Poland. Electronic address: kamil.myszczynski@gumed.edu.pl., Domżalski P; Department of Histology, Medical University of Gdansk, 1a Dębinki, 80-211 Gdansk, Poland. Electronic address: pawel.domzalski@gumed.edu.pl., Żmijewski MA; Department of Histology, Medical University of Gdansk, 1a Dębinki, 80-211 Gdansk, Poland. Electronic address: mzmijewski@gumed.edu.pl.
المصدر: Steroids [Steroids] 2023 Nov; Vol. 199, pp. 109288. Date of Electronic Publication: 2023 Aug 05.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 0404536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-5867 (Electronic) Linking ISSN: 0039128X NLM ISO Abbreviation: Steroids Subsets: MEDLINE
أسماء مطبوعة: Publication: New York Ny : Elsevier
Original Publication: San Francisco.
مواضيع طبية MeSH: Carcinoma, Squamous Cell*/genetics , Protein Disulfide-Isomerases*/genetics , Protein Disulfide-Isomerases*/metabolism , Vitamin D*/metabolism, Humans ; Genomics ; Receptors, Calcitriol/genetics ; TRPV Cation Channels/metabolism
مستخلص: An active form of vitamin D 3 (1,25-dihydroxyvitamin D 3 ) acts through vitamin D receptor (VDR) initiating genomic response, but several studies described also non-genomic actions of 1,25-dihydroxyvitamin D 3 , implying the role of PDIA3 in the process. PDIA3 is a membrane-associated disulfide isomerase involved in disulfide bond formation, protein folding, and remodeling. Here, we used a transcriptome-based approach to identify changes in expression profiles in PDIA3-deficient squamous cell carcinoma line A431 after 1,25-dihydroxyvitamin D 3 treatment. PDIA3 knockout led to changes in the expression of more than 2000 genes and modulated proliferation, cell cycle, and mobility of cells; suggesting an important regulatory role of PDIA3. PDIA3-deficient cells showed increased sensitivity to 1,25-dihydroxyvitamin D 3 , which led to decrease migration. 1,25-dihydroxyvitamin D 3 treatment altered also genes expression profile of A431ΔPDIA3 in comparison to A431WT cells, indicating the existence of PDIA3-dependent genes. Interestingly, classic targets of VDR, including CAMP (Cathelicidin Antimicrobial Peptide), TRPV6 (Transient Receptor Potential Cation Channel Subfamily V Member 6), were regulated differently by 1,25-dihydroxyvitamin D 3 , in A431ΔPDIA3. Deletion of PDIA3 impaired 1,25-dihydroxyvitamin D 3 -response of genes, such as PTGS2, MMP12, and FOCAD, which were identified as PDIA3-dependent. Additionally, response to 1,25-dihydroxyvitamin D 3 in cancerous A431 cells differed from immortalized HaCaT keratinocytes, used as non-cancerous control. Finally, silencing of PDIA3 and 1,25-dihydroxyvitamin D 3 , at least partially reverse the expression of cancer-related genes in A431 cells, thus targeting PDIA3 and use of 1,25-dihydroxyvitamin D 3 could be considered in a prevention and therapy of the skin cancer. Taken together, PDIA3 has a strong impact on gene expression and physiology, including genomic response to 1,25-dihydroxyvitamin D 3 .
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: 1,25-dihydroxyvitaminvitamin D3; PDIA3; Squamous cell carcinoma; Transcriptome
المشرفين على المادة: 66772-14-3 (1,25-dihydroxyvitamin D)
EC 5.3.4.1. (PDIA3 protein, human)
EC 5.3.4.1 (Protein Disulfide-Isomerases)
0 (Receptors, Calcitriol)
1406-16-2 (Vitamin D)
0 (TRPV6 channel)
0 (TRPV Cation Channels)
تواريخ الأحداث: Date Created: 20230807 Date Completed: 20231012 Latest Revision: 20231012
رمز التحديث: 20231012
DOI: 10.1016/j.steroids.2023.109288
PMID: 37549780
قاعدة البيانات: MEDLINE
الوصف
تدمد:1878-5867
DOI:10.1016/j.steroids.2023.109288