دورية أكاديمية
SARS-CoV-2 RNA and N Antigen Quantification via Wastewater at the Campus Level, Building Cluster Level, and Individual-Building Level.
| العنوان: | SARS-CoV-2 RNA and N Antigen Quantification via Wastewater at the Campus Level, Building Cluster Level, and Individual-Building Level. |
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| المؤلفون: | Zambrana W; Department of Civil and Environmental Engineering, Stanford University, 473 Via Ortega, Stanford, California 94305, United States., Catoe D; Joint Initiative for Metrology in Biology, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, United States., Coffman MM; Department of Civil and Environmental Engineering, Stanford University, 473 Via Ortega, Stanford, California 94305, United States., Kim S; Department of Civil and Environmental Engineering, Stanford University, 473 Via Ortega, Stanford, California 94305, United States., Anand A; Department of Civil and Environmental Engineering, Stanford University, 473 Via Ortega, Stanford, California 94305, United States., Solis D; Department of Pathology, Stanford University School of Medicine, Stanford, California 94305, United States., Sahoo MK; Department of Pathology, Stanford University School of Medicine, Stanford, California 94305, United States., Pinsky BA; Department of Pathology, Stanford University School of Medicine, Stanford, California 94305, United States.; Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California 94305, United States., Bhatt AS; Department of Medicine (Hematology) and Department of Genetics, Stanford University, Stanford, California 94305, United States., Boehm AB; Department of Civil and Environmental Engineering, Stanford University, 473 Via Ortega, Stanford, California 94305, United States., Wolfe MK; Department of Civil and Environmental Engineering, Stanford University, 473 Via Ortega, Stanford, California 94305, United States.; Gangarosa Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, Georgia 30322, United States. |
| المصدر: | ACS ES&T water [ACS ES T Water] 2022 May 12; Vol. 2 (11), pp. 2025-2033. Date of Electronic Publication: 2022 May 12 (Print Publication: 2022). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101778136 Publication Model: eCollection Cited Medium: Internet ISSN: 2690-0637 (Electronic) Linking ISSN: 26900637 NLM ISO Abbreviation: ACS ES T Water Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, District of Columbia : American Chemical Society, [2021]- |
| مستخلص: | Monitoring wastewater for SARS-CoV-2 from populations smaller than those served by wastewater treatment plants may help identify small spatial areas (subsewersheds) where COVID-19 infections are present. We sampled wastewater from three nested locations with different sized populations within the same sewer network at a university campus and quantified SARS-CoV-2 RNA using reverse transcriptase droplet digital polymerase chain reaction (PCR). SARS-CoV-2 RNA concentrations and/or concentrations normalized by PMMoV were positively associated with laboratory-confirmed COVID-19 cases for both the sewershed level and the subsewershed level. We also used an antigen-based assay to detect the nucleocapsid (N) antigen from SARS-CoV-2 in wastewater samples at the sewershed level. The N antigen was regularly detected at the sewershed level, but the results were not associated with either laboratory-confirmed COVID-19 cases or SARS-CoV-2 RNA concentrations. The results of this study indicate that wastewater monitoring based on quantification of SARS-CoV-2 RNA using PCR-based methods is associated with COVID-19 cases at multiple geographic scales within the subsewershed level and can serve to aid the public health response. Competing Interests: The authors declare no competing financial interest. (© 2022 American Chemical Society.) |
| تواريخ الأحداث: | Date Created: 20230808 Latest Revision: 20230829 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1021/acsestwater.2c00050 |
| PMID: | 37552722 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 2690-0637 |
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| DOI: | 10.1021/acsestwater.2c00050 |