دورية أكاديمية
In vitro evaluation of antioxidant, cytotoxic, trypanocidal and antimicrobial activities of lignin obtained from Caesalpinia ferrea leaves and its use as an excipient in the release of oxacillin and fluconazole.
| العنوان: | In vitro evaluation of antioxidant, cytotoxic, trypanocidal and antimicrobial activities of lignin obtained from Caesalpinia ferrea leaves and its use as an excipient in the release of oxacillin and fluconazole. |
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| المؤلفون: | da Silva BRMG; Department of Antibiotics, Laboratory of Chemistry and Therapeutic Innovation, Federal of Pernambuco, 50670-901, Brazil., Arruda POF; Department of Antibiotics, Laboratory of Chemistry and Therapeutic Innovation, Federal of Pernambuco, 50670-901, Brazil., Dos Santos JCB; Department of Antibiotics, Laboratory of Chemistry and Therapeutic Innovation, Federal of Pernambuco, 50670-901, Brazil., Neves RP; Medical Mycology Laboratory, Federal University of Pernambuco, 50670-901, Brazil., Pereira VRA; Oswaldo Cruz Pernambuco Foundation (Fiocruz/PE), Department of Immunology, 50670-420 Recife, PE, Brazil., de Lima MCA; Department of Antibiotics, Laboratory of Chemistry and Therapeutic Innovation, Federal of Pernambuco, 50670-901, Brazil., de Araújo HDA; Laboratory of Immunopathology and Infectious-Parasitic Diseases Keizo Asami - LIKA, 50670-420 Recife, PE, Brazil., Marques DSC; Department of Antibiotics, Laboratory of Chemistry and Therapeutic Innovation, Federal of Pernambuco, 50670-901, Brazil., da Cruz Filho IJ; Department of Antibiotics, Laboratory of Chemistry and Therapeutic Innovation, Federal of Pernambuco, 50670-901, Brazil. Electronic address: iranildoj@gmail.com. |
| المصدر: | International journal of biological macromolecules [Int J Biol Macromol] 2023 Oct 01; Vol. 250, pp. 126225. Date of Electronic Publication: 2023 Aug 07. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Guildford, Eng., IPC Science and Technology Press. |
| مستخلص: | In this context, the objective of this work was to isolate an alkaline lignin from the leaves of C. ferrea, in addition to investigating different biological activities and its use in the production of releasing tablets in vitro. Initially, the analysis of the composition of the leaves was performed, the contents were: cellulose (33.09 ± 0.3 %), hemicellulose (25.13 ± 0.1 %), lignin (18.29 ± 0.1 %), extractives (17.28 ± 1.0 %) and ash (6.20 ± 0.1 %). The leaves were fractionated to obtain alkaline lignin. The yield of obtaining lignin was 80.12 ± 0.1 %. The obtained lignin was characterized by the techniques: elemental analysis, FTIR, UV/Vis, 2D-NMR, GPC, TGA/DTG, DSC and PY-GC/MS. The results showed that the lignin obtained is of the GSH type, of low molecular weight and thermally stable. The in vitro antioxidant activity was evaluated by different assays promoting results only for DPPH (559.9 ± 0.8 μg/mL) and ABTS (484.1 ± 0.1 μg/mL) being able to promote low antioxidant activity. In addition, it showed low cytotoxicity in normal mammalian cells and promising antitumor and trypanocidal activity. Regarding antimicrobial activity, it was able to inhibit the growth of a strain of Staphylococcus aureus resistant to methicillin, presenting MIC values equal to the standard antibiotic oxacillin. It was also able to inhibit a strain of Candida albicans HAM13 sensitive to fluconazole. In addition, lignin promoted a synergistic effect by promoting a decrease in MIC against these two strains evaluated. Finally, lignin proved to be an excipient with potential for controlled release of antimicrobials. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could appear to influence the work reported in this article. (Copyright © 2023. Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: Alkaline lignin; Caesalpinia ferrea; Candida albicans; Drug release; Staphylococcus aureus |
| تواريخ الأحداث: | Date Created: 20230809 Latest Revision: 20231017 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.ijbiomac.2023.126225 |
| PMID: | 37558029 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0003 |
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| DOI: | 10.1016/j.ijbiomac.2023.126225 |