دورية أكاديمية
Kaempferol from Moringa oleifera demonstrated potent antivenom activities via inhibition of metalloproteinase and attenuation of Bitis arietans venom-induced toxicities.
| العنوان: | Kaempferol from Moringa oleifera demonstrated potent antivenom activities via inhibition of metalloproteinase and attenuation of Bitis arietans venom-induced toxicities. |
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| المؤلفون: | Ajisebiola BS; Department of Zoology, Osun State University, Osogbo, Nigeria; Animal Physiology Unit, Department of Zoology, University of Ibadan, Ibadan, Nigeria. Electronic address: dbabslinks@yahoo.com., Oladele JO; Biochemistry Unit, Department of Chemical Sciences, Kings University, Ode-Omu, Nigeria., Adeyi AO; Animal Physiology Unit, Department of Zoology, University of Ibadan, Ibadan, Nigeria. Electronic address: delegenius@yahoo.com. |
| المصدر: | Toxicon : official journal of the International Society on Toxinology [Toxicon] 2023 Sep; Vol. 233, pp. 107242. Date of Electronic Publication: 2023 Aug 08. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pergamon Press Country of Publication: England NLM ID: 1307333 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3150 (Electronic) Linking ISSN: 00410101 NLM ISO Abbreviation: Toxicon Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Oxford ; New York : Pergamon Press, c1962- |
| مواضيع طبية MeSH: | Moringa oleifera* , Snake Bites*/drug therapy, Animals ; Rats ; Antivenins/pharmacology ; Antivenins/therapeutic use ; Kaempferols/pharmacology ; Kaempferols/therapeutic use ; Snake Venoms ; Metalloproteases/therapeutic use |
| مستخلص: | Bitis arietans venom (BAV) can induce severe pathophysiological disorders after envenoming. However, studies have shown that the Moringa oleifera fraction is effective against BAV toxicities and contains bioactive compounds with significant antivenom potency. This research aimed to identify the main active antivenom compound in the M. oleifera fraction responsible for neutralizing the toxicities induced by BAV. The compounds identified from M. oleifera fraction were docked in silico against the catalytic site of the Snake Venom Metalloproteinase (SVMP) to determine the lead inhibitor compound. The antivenom potency of the lead inhibitor compound was tested against BAV toxicities and metalloproteinase isolated from BAV using in vitro and in vivo methods, while EchiTab-Plus polyvalent antivenom served as a standard drug. The in silico prediction revealed kaempferol as the lead inhibitor compound with a docking score of -7.0 kcal/mol. Kaempferol effectively inhibited metalloproteinase activity at 0.2 mg/ml, compared to antivenom (0.4 mg/ml) and demonstrated significant antihaemorrhagic, antihaemolytic and coagulant effects against BAV activities. Furthermore, kaempferol showed a significant dose-dependent effect on altered haematological indices observed in rats challenged with LD Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Bitis arietans; Envenomation; Kaempferol; Metalloproteinase; Moringa oleifera; Natural inhibitor |
| المشرفين على المادة: | 0 (Antivenins) 0 (Kaempferols) 0 (Snake Venoms) EC 3.4.- (Metalloproteases) |
| SCR Organism: | Bitis arietans |
| تواريخ الأحداث: | Date Created: 20230809 Date Completed: 20230920 Latest Revision: 20230920 |
| رمز التحديث: | 20230920 |
| DOI: | 10.1016/j.toxicon.2023.107242 |
| PMID: | 37558138 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-3150 |
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| DOI: | 10.1016/j.toxicon.2023.107242 |