دورية أكاديمية
A germ-free humanized mouse model shows the contribution of resident microbiota to human-specific pathogen infection.
| العنوان: | A germ-free humanized mouse model shows the contribution of resident microbiota to human-specific pathogen infection. |
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| المؤلفون: | Wahl A; International Center for the Advancement of Translational Science, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. awahl@med.unc.edu.; Division of Infectious Diseases, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. awahl@med.unc.edu.; Center for AIDS Research, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. awahl@med.unc.edu., Yao W; International Center for the Advancement of Translational Science, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Division of Infectious Diseases, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Center for AIDS Research, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Liao B; International Center for the Advancement of Translational Science, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Division of Infectious Diseases, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Center for AIDS Research, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou, China., Chateau M; International Center for the Advancement of Translational Science, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Division of Infectious Diseases, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Center for AIDS Research, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Richardson C; International Center for the Advancement of Translational Science, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Division of Infectious Diseases, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Center for AIDS Research, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Ling L; International Center for the Advancement of Translational Science, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Division of Infectious Diseases, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Center for AIDS Research, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Franks A; International Center for the Advancement of Translational Science, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Division of Infectious Diseases, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Center for AIDS Research, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Senthil K; International Center for the Advancement of Translational Science, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Division of Infectious Diseases, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Center for AIDS Research, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Doyon G; International Center for the Advancement of Translational Science, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Division of Infectious Diseases, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Center for AIDS Research, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Li F; Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Frost J; Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Division of Comparative Medicine, Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Whitehurst CB; Department of Pathology, Microbiology, and Immunology, New York Medical College, Valhalla, NY, USA., Pagano JS; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Fletcher CA; Division of Comparative Medicine, Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Azcarate-Peril MA; Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Division of Gastroenterology and Hepatology, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; UNC Microbiome Core, University of North Carolina, Chapel Hill, NC, USA., Hudgens MG; Department of Biostatistics, Gillings School of Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Rogala AR; Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Division of Comparative Medicine, Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Tucker JD; Division of Infectious Diseases, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Clinical Research Department, Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK., McGowan I; Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, University of Pittsburgh Medical School, Pittsburgh, PA, USA.; Orion Biotechnology, Ottawa, Ontario, Canada., Sartor RB; Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Division of Gastroenterology and Hepatology, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Garcia JV; International Center for the Advancement of Translational Science, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. victor_garcia@med.unc.edu.; Division of Infectious Diseases, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. victor_garcia@med.unc.edu.; Center for AIDS Research, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. victor_garcia@med.unc.edu.; Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. victor_garcia@med.unc.edu. |
| المصدر: | Nature biotechnology [Nat Biotechnol] 2024 Jun; Vol. 42 (6), pp. 905-915. Date of Electronic Publication: 2023 Aug 10. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature America Publishing Country of Publication: United States NLM ID: 9604648 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1546-1696 (Electronic) Linking ISSN: 10870156 NLM ISO Abbreviation: Nat Biotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York Ny : Nature America Publishing Original Publication: New York, NY : Nature Pub. Co., [1996- |
| مواضيع طبية MeSH: | Disease Models, Animal* , Microbiota* , Germ-Free Life* , HIV Infections*/immunology , HIV Infections*/microbiology, Animals ; Humans ; Mice ; Epstein-Barr Virus Infections/immunology ; Herpesvirus 4, Human/immunology ; CD4-Positive T-Lymphocytes/immunology ; HIV ; Gastrointestinal Microbiome |
| مستخلص: | Germ-free (GF) mice, which are depleted of their resident microbiota, are the gold standard for exploring the role of the microbiome in health and disease; however, they are of limited value in the study of human-specific pathogens because they do not support their replication. Here, we develop GF mice systemically reconstituted with human immune cells and use them to evaluate the role of the resident microbiome in the acquisition, replication and pathogenesis of two human-specific pathogens, Epstein-Barr virus (EBV) and human immunodeficiency virus (HIV). Comparison with conventional (CV) humanized mice showed that resident microbiota enhance the establishment of EBV infection and EBV-induced tumorigenesis and increase mucosal HIV acquisition and replication. HIV RNA levels were higher in plasma and tissues of CV humanized mice compared with GF humanized mice. The frequency of CCR5 + CD4 + T cells throughout the intestine was also higher in CV humanized mice, indicating that resident microbiota govern levels of HIV target cells. Thus, resident microbiota promote the acquisition and pathogenesis of two clinically relevant human-specific pathogens. (© 2023. The Author(s), under exclusive licence to Springer Nature America, Inc.) |
| References: | Annu Rev Virol. 2014;1:55-69. (PMID: 25821837) Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4516-22. (PMID: 20534432) PLoS One. 2013 May 01;8(5):e62578. (PMID: 23658749) Curr HIV Res. 2020;18(1):19-28. (PMID: 31870268) Clin Transl Immunology. 2017 Feb 10;6(2):e128. (PMID: 28243438) Nat Med. 2006 Dec;12(12):1365-71. (PMID: 17115046) J Virol. 2011 Aug;85(15):7582-93. (PMID: 21593172) Retrovirology. 2013 Oct 24;10:121. (PMID: 24156277) PLoS One. 2012;7(11):e49526. (PMID: 23185351) Semin Cancer Biol. 1992 Oct;3(5):297-307. (PMID: 1335793) Front Immunol. 2019 Feb 06;10:143. (PMID: 30787928) Antimicrob Agents Chemother. 2015 Dec;59(12):7847-51. (PMID: 26392489) AIDS. 2001 Sep 7;15(13):1627-34. (PMID: 11546936) J Clin Invest. 2018 Jul 2;128(7):2862-2876. (PMID: 29863499) Proc Natl Acad Sci U S A. 1998 Jul 21;95(15):8886-91. (PMID: 9671774) Nat Rev Immunol. 2004 Jun;4(6):478-85. (PMID: 15173836) Cancer Res. 2000 Oct 15;60(20):5781-8. (PMID: 11059774) Lancet. 1991 Oct 19;338(8773):969-73. (PMID: 1681341) PLoS Pathog. 2014 Aug 28;10(8):e1004333. (PMID: 25165855) Immunology. 1993 May;79(1):32-7. (PMID: 8509140) PLoS One. 2013;8(3):e60024. (PMID: 23527295) Nat Commun. 2020 Apr 29;11(1):2097. (PMID: 32350281) mBio. 2014 Apr 29;5(3):e01015-14. (PMID: 24781742) Nature. 2014 Dec 4;516(7529):94-8. (PMID: 25409145) mBio. 2019 Jan 15;10(1):. (PMID: 30647153) Front Microbiol. 2011 May 26;2:111. (PMID: 21833339) Nat Methods. 2016 Jul;13(7):581-3. (PMID: 27214047) J Immunol. 2010 Feb 15;184(4):2026-37. (PMID: 20089703) Nat Biotechnol. 2019 Oct;37(10):1163-1173. (PMID: 31451733) Science. 2011 Oct 14;334(6053):245-9. (PMID: 21998394) Proc Natl Acad Sci U S A. 1998 Jul 7;95(14):8245-50. (PMID: 9653172) Cancer Res. 1999 Apr 1;59(7):1485-91. (PMID: 10197618) Science. 2011 Oct 14;334(6053):249-52. (PMID: 21998395) Front Virol. 2022;2:. (PMID: 35611388) Nat Commun. 2019 Dec 11;10(1):5656. (PMID: 31827089) J Exp Med. 2007 Apr 16;204(4):705-14. (PMID: 17389241) J Neurol Neurosurg Psychiatry. 2012 Mar;83(3):353-4. (PMID: 21791511) EBioMedicine. 2016 Jan 26;4:40-9. (PMID: 26981570) J Virol. 2012 Jan;86(1):630-4. (PMID: 22013053) Nature. 2020 Feb;578(7793):154-159. (PMID: 31969705) Sci Rep. 2018 Sep 21;8(1):14184. (PMID: 30242285) PLoS Pathog. 2014 Feb 20;10(2):e1003829. (PMID: 24586144) J Immunol. 1998 Sep 15;161(6):3195-201. (PMID: 9743388) AIMS Microbiol. 2019 Mar 22;5(1):102-116. (PMID: 31384706) J Vis Exp. 2019 Aug 30;(150):. (PMID: 31524867) Front Immunol. 2020 Feb 18;11:214. (PMID: 32133003) PLoS Pathog. 2012;8(5):e1002681. (PMID: 22615558) Science. 2014 Nov 7;346(6210):755-9. (PMID: 25378626) J Virol. 2004 Dec;78(23):12987-95. (PMID: 15542650) PLoS One. 2010 Jan 21;5(1):e8829. (PMID: 20098623) J Virol. 2015 Nov;89(21):10868-78. (PMID: 26292320) Nat Med. 2017 Nov;23(11):1271-1276. (PMID: 28967921) Front Physiol. 2018 Oct 31;9:1534. (PMID: 30429801) Clin Immunol. 1999 Jun;91(3):254-62. (PMID: 10370370) BMC Microbiol. 2017 Sep 13;17(1):194. (PMID: 28903732) BMC Bioinformatics. 2019 Nov 15;20(1):581. (PMID: 31730472) Cell Host Microbe. 2014 Jan 15;15(1):36-46. (PMID: 24439896) Cell. 2012 Jun 22;149(7):1578-93. (PMID: 22726443) Sci Rep. 2017 Feb 01;7:41098. (PMID: 28145472) mSphere. 2021 Oct 27;6(5):e0075121. (PMID: 34585958) Nature. 2020 Feb;578(7793):160-165. (PMID: 31969707) Nature. 2021 Mar;591(7850):451-457. (PMID: 33561864) J Virol. 2003 Nov;77(21):11708-17. (PMID: 14557656) N Engl J Med. 1989 Feb 23;320(8):502-6. (PMID: 2536894) J Clin Immunol. 2001 Mar;21(2):116-25. (PMID: 11332650) BMC Infect Dis. 2014 Dec 13;14:683. (PMID: 25495598) J Virol. 2005 Apr;79(8):4828-37. (PMID: 15795268) Nat Med. 2006 Nov;12(11):1316-22. (PMID: 17057712) Sci Transl Med. 2011 Nov 2;3(107):107fs7. (PMID: 22049067) J Antimicrob Chemother. 2016 Nov;71(11):3185-3194. (PMID: 27494916) Nature. 1970 Dec 12;228(5276):1056-8. (PMID: 4320657) J Clin Invest. 2016 Mar 1;126(3):892-904. (PMID: 26854925) J Infect Dis. 2000 Mar;181(3):933-8. (PMID: 10720515) J Immunol. 1999 Oct 15;163(8):4597-603. (PMID: 10510404) PLoS Biol. 2016 Aug 19;14(8):e1002533. (PMID: 27541692) Front Cell Infect Microbiol. 2022 Feb 23;12:852066. (PMID: 35281433) Mucosal Immunol. 2012 Sep;5(5):555-66. (PMID: 22569301) J Clin Invest. 2016 Apr 1;126(4):1353-66. (PMID: 26950420) Science. 2015 Jan 16;347(6219):266-9. (PMID: 25431490) Nat Immunol. 2013 Jul;14(7):676-84. (PMID: 23778795) Mucosal Immunol. 2008 Jan;1(1):23-30. (PMID: 19079157) J Virol. 2014 Jun;88(11):6411-22. (PMID: 24672041) Curr Opin HIV AIDS. 2021 Jul 1;16(4):200-208. (PMID: 34039843) Appl Environ Microbiol. 2018 Feb 14;84(5):. (PMID: 29269490) Microbiome. 2018 May 17;6(1):90. (PMID: 29773078) J Infect Dis. 2000 Mar;181(3):927-32. (PMID: 10720514) Exp Mol Med. 2020 Sep;52(9):1383-1396. (PMID: 32908211) Biochimie. 2012 Mar;94(3):839-46. (PMID: 22178321) Gut Microbes. 2013 Sep-Oct;4(5):361-70. (PMID: 23887190) PLoS One. 2016 Jul 20;11(7):e0159517. (PMID: 27438728) Mucosal Immunol. 2014 Jul;7(4):983-94. (PMID: 24399150) Clin Microbiol Rev. 2011 Jan;24(1):193-209. (PMID: 21233512) Nat Rev Immunol. 2009 May;9(5):313-23. (PMID: 19343057) Nat Commun. 2022 Jan 26;13(1):521. (PMID: 35082297) Immunology. 2014 Aug;142(4):562-72. (PMID: 24409837) PLoS Pathog. 2012;8(6):e1002732. (PMID: 22737068) Proc Natl Acad Sci U S A. 2014 Feb 25;111(8):3146-51. (PMID: 24516154) J Virol. 2012 Mar;86(5):2715-28. (PMID: 22190722) N Engl J Med. 2001 Dec 27;345(26):1877-82. (PMID: 11756578) J Virol. 2013 May;87(10):5437-46. (PMID: 23468485) Cell. 2009 Oct 30;139(3):485-98. (PMID: 19836068) Cell Rep. 2013 Jun 27;3(6):1874-84. (PMID: 23791525) |
| معلومات مُعتمدة: | U19 AI082637 United States AI NIAID NIH HHS; UM1 AI126619 United States AI NIAID NIH HHS; P30 DK034987 United States DK NIDDK NIH HHS; P30 CA016086 United States CA NCI NIH HHS; P30DK034987 U.S. Department of Health & Human Services | National Institutes of Health (NIH); DK131585 U.S. Department of Health & Human Services | National Institutes of Health (NIH); D43 TW009532 United States TW FIC NIH HHS; AI123010 U.S. Department of Health & Human Services | National Institutes of Health (NIH); P30 DK056350 United States DK NIDDK NIH HHS; 1UM1AI126619 U.S. Department of Health & Human Services | National Institutes of Health (NIH); P30 AI050410 United States AI NIAID NIH HHS; R34 MH119963 United States MH NIMH NIH HHS; U19AI082637 U.S. Department of Health & Human Services | National Institutes of Health (NIH); P40 OD010995 United States OD NIH HHS; P40OD010995 U.S. Department of Health & Human Services | National Institutes of Health (NIH); D43TW009532 U.S. Department of Health & Human Services | NIH | Fogarty International Center (FIC); R01 AI123010 United States AI NIAID NIH HHS; UM1 AI164567 United States AI NIAID NIH HHS; R01 DK131585 United States DK NIDDK NIH HHS |
| تواريخ الأحداث: | Date Created: 20230810 Date Completed: 20240616 Latest Revision: 20240718 |
| رمز التحديث: | 20240718 |
| مُعرف محوري في PubMed: | PMC11073568 |
| DOI: | 10.1038/s41587-023-01906-5 |
| PMID: | 37563299 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1546-1696 |
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| DOI: | 10.1038/s41587-023-01906-5 |