دورية أكاديمية
Optimization of fecal sample homogenization for untargeted metabolomics.
العنوان: | Optimization of fecal sample homogenization for untargeted metabolomics. |
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المؤلفون: | Tarazona Carrillo K; Department of Chemistry, University of Alberta, Edmonton, AB, T6G 2G2, Canada., Nam SL; Department of Chemistry, University of Alberta, Edmonton, AB, T6G 2G2, Canada., de la Mata AP; Department of Chemistry, University of Alberta, Edmonton, AB, T6G 2G2, Canada., de Bruin OM; DNA Genotek, Ottawa, ON, Canada., Doukhanine E; DNA Genotek, Ottawa, ON, Canada., Harynuk J; Department of Chemistry, University of Alberta, Edmonton, AB, T6G 2G2, Canada. james.harynuk@ualberta.ca. |
المصدر: | Metabolomics : Official journal of the Metabolomic Society [Metabolomics] 2023 Aug 11; Vol. 19 (8), pp. 74. Date of Electronic Publication: 2023 Aug 11. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Springer Country of Publication: United States NLM ID: 101274889 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-3890 (Electronic) Linking ISSN: 15733882 NLM ISO Abbreviation: Metabolomics Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: New York : Springer, c2006- |
مواضيع طبية MeSH: | Metabolomics*/methods , Metabolome*, Reproducibility of Results ; Feces ; Solvents |
مستخلص: | Introduction: Fecal samples are highly complex and heterogeneous, containing materials at various stages of digestion. The heterogeneity and complexity of feces make stool metabolomics inherently challenging. The level of homogenization influences the outcome of the study, affecting the metabolite profiles and reproducibility; however, there is no consensus on how fecal samples should be prepared to overcome the topographical discrepancy and obtain data representative of the stool as a whole. Objectives: Various combinations of homogenization conditions were compared to investigate the effects of bead size, addition of solvents and the differences between wet-frozen and lyophilized feces. Methods: The homogenization parameters were systematically altered to evaluate the solvent usage, bead size, and whether lyophilization is required in homogenization. The metabolic coverage and reproducibility were compared among the different conditions. Results: The current work revealed that a combination of mechanical and chemical lysis obtained by bead-beating with a mixture of big and small sizes of beads in an organic solvent is an effective way to homogenize fecal samples with adequate reproducibility and metabolic coverage. Lyophilization is required when bead-beating is not available. Conclusions: A comprehensive and systematical evaluation of various fecal matter homogenization conditions provides a profound understanding for the effects of different homogenization methods. Our findings would be beneficial to assist with standardization of fecal sample homogenization protocol. (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.) |
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فهرسة مساهمة: | Keywords: Bead-beating; Feces; GC × GC-TOFMS; Homogenization; Lyophilization; Metabolomics; Microbiome |
المشرفين على المادة: | 0 (Solvents) |
تواريخ الأحداث: | Date Created: 20230811 Date Completed: 20230814 Latest Revision: 20230821 |
رمز التحديث: | 20240628 |
DOI: | 10.1007/s11306-023-02036-4 |
PMID: | 37566260 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1573-3890 |
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DOI: | 10.1007/s11306-023-02036-4 |