دورية أكاديمية
أعرض في EDS

Fighting Pseudomonas aeruginosa Infections: Antibacterial and Antibiofilm Activity of D-Q53 CecB, a Synthetic Analog of a Silkworm Natural Cecropin B Variant.

التفاصيل البيبلوغرافية
العنوان: Fighting Pseudomonas aeruginosa Infections: Antibacterial and Antibiofilm Activity of D-Q53 CecB, a Synthetic Analog of a Silkworm Natural Cecropin B Variant.
المؤلفون: Varponi I; Department of Biology, University of Padova, Via U. Bassi 58/B, 35131 Padova, Italy., Ferro S; Department of Biomedical Sciences, University of Padova, Via U. Bassi 58/B, 35131 Padova, Italy., Menilli L; Department of Biology, University of Padova, Via U. Bassi 58/B, 35131 Padova, Italy., Grapputo A; Department of Biology, University of Padova, Via U. Bassi 58/B, 35131 Padova, Italy.; National Biodiversity Future Centre, Piazza Marina 61, 90133 Palermo, Italy., Moret F; Department of Biology, University of Padova, Via U. Bassi 58/B, 35131 Padova, Italy., Mastrotto F; Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via F. Marzolo 5, 35131 Padova, Italy., Marin O; Department of Biomedical Sciences, University of Padova, Via U. Bassi 58/B, 35131 Padova, Italy., Sandrelli F; Department of Biology, University of Padova, Via U. Bassi 58/B, 35131 Padova, Italy.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2023 Aug 06; Vol. 24 (15). Date of Electronic Publication: 2023 Aug 06.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Cecropins*/pharmacology , Cecropins*/therapeutic use , Pseudomonas Infections*/drug therapy , Pseudomonas Infections*/microbiology, Animals ; Humans ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/chemistry ; Biofilms/drug effects ; Bombyx ; Microbial Sensitivity Tests ; Pseudomonas aeruginosa/drug effects
مستخلص: Pseudomonas aeruginosa is an opportunistic Gram-negative bacterium responsible for severe nosocomial infections and is considered a critical pulmonary pathogen for both immunocompromised and cystic fibrosis patients. Planktonic cells of P. aeruginosa possess intrinsic and acquired resistances, inactivating several classes of conventional antibiotics. Additionally, this bacterium can grow, forming biofilms, and complex structures, further hampering the action of multiple antibiotics. Here, we report the biological properties of D-Q53 CecB, an all-D enantiomer of the silkworm natural peptide Q53 CecB. Compared to the L-variant, D-Q53 CecB was resistant to in vitro degradation by humans and P. aeruginosa elastases and showed an enhanced bactericidal activity against P. aeruginosa planktonic bacteria. D-Q53 CecB was thermostable and maintained its antimicrobial activity at high salt concentrations and in the presence of divalent cations or fetal-bovine serum, although at reduced levels. Against different types of human cells, D-Q53 CecB showed cytotoxic phenomena at concentrations several folds higher compared to those active against P. aeruginosa. When L- and D-Q53 CecB were compared for their antibiofilm properties, both peptides were active in inhibiting biofilm formation. However, the D-enantiomer was extremely effective in inducing biofilm degradation, suggesting this peptide as a favorable candidate in an anti- Pseudomonas therapy.
References: Contemp Clin Dent. 2015 Oct-Dec;6(4):461-5. (PMID: 26681848)
Pulm Pharmacol Ther. 2008 Aug;21(4):595-9. (PMID: 18234534)
FEMS Microbiol Lett. 2008 Aug;285(1):40-50. (PMID: 18513356)
Methods Mol Biol. 2017;1548:309-322. (PMID: 28013514)
Sci Rep. 2020 Apr 10;10(1):6232. (PMID: 32277108)
Int J Pept Protein Res. 1990 Mar;35(3):161-214. (PMID: 2191922)
Chem Soc Rev. 2021 Apr 26;50(8):4932-4973. (PMID: 33710195)
Environ Microbiol. 2013 Oct;15(10):2865-78. (PMID: 23751003)
Antimicrob Agents Chemother. 2008 Jan;52(1):85-91. (PMID: 17954700)
Mol Microbiol. 2009 Dec;74(6):1380-92. (PMID: 19889094)
Curr Med Chem. 2022;29(25):4307-4310. (PMID: 34979887)
Proc Natl Acad Sci U S A. 1990 Jun;87(12):4761-5. (PMID: 1693777)
Sci Rep. 2019 Apr 24;9(1):6496. (PMID: 31019225)
Am Rev Respir Dis. 1993 Dec;148(6 Pt 1):1633-7. (PMID: 8256912)
Int J Mol Sci. 2021 Aug 23;22(16):. (PMID: 34445806)
Mol Microbiol. 2014 May;92(3):488-506. (PMID: 24655293)
PLoS Pathog. 2008 Nov;4(11):e1000213. (PMID: 19023416)
Mol Cell Biochem. 2001 Feb;218(1-2):105-11. (PMID: 11330824)
Clin Microbiol Rev. 2004 Jan;17(1):57-71. (PMID: 14726455)
Environ Microbiol. 2015 Nov;17(11):4148-63. (PMID: 25808785)
Profiles Drug Subst Excip Relat Methodol. 2021;46:273-307. (PMID: 33461699)
J Appl Microbiol. 2001 Jul;91(1):29-37. (PMID: 11442711)
NPJ Biofilms Microbiomes. 2019 May 23;5(1):15. (PMID: 31149345)
Front Microbiol. 2018 May 08;9:855. (PMID: 29867793)
Mol Microbiol. 2010 Oct;78(1):158-72. (PMID: 20735777)
Front Microbiol. 2011 Aug 22;2:167. (PMID: 21991261)
Future Med Chem. 2015;7(4):493-512. (PMID: 25875875)
Amino Acids. 2015 Dec;47(12):2505-19. (PMID: 26162435)
Appl Environ Microbiol. 2013 Dec;79(23):7116-21. (PMID: 24038684)
Ann Am Thorac Soc. 2016 Apr;13 Suppl 2:S156-62. (PMID: 27115951)
Antimicrob Agents Chemother. 1972 Jun;1(6):466-9. (PMID: 4218943)
Sci Rep. 2017 Jul 31;7(1):6953. (PMID: 28761101)
Microb Pathog. 2016 Sep;98:45-9. (PMID: 27354207)
Front Cell Infect Microbiol. 2022 Feb 28;12:851784. (PMID: 35295755)
Proc Biol Sci. 2018 Mar 14;285(1874):. (PMID: 29540517)
Biomol Concepts. 2016 Jun 1;7(3):179-87. (PMID: 27159920)
Clin Microbiol Rev. 2020 May 13;33(3):. (PMID: 32404435)
Nat Rev Microbiol. 2010 Sep;8(9):634-44. (PMID: 20694026)
Front Chem. 2017 Jun 19;5:40. (PMID: 28674688)
Antimicrob Agents Chemother. 2003 Jan;47(1):317-23. (PMID: 12499208)
J Bacteriol. 2012 Aug;194(16):4285-94. (PMID: 22685276)
Exp Cell Res. 1998 Sep 15;243(2):359-66. (PMID: 9743595)
Biotechnol Adv. 2019 Jan - Feb;37(1):177-192. (PMID: 30500353)
Nature. 2005 Aug 25;436(7054):1171-5. (PMID: 16121184)
Front Microbiol. 2019 Dec 11;10:2854. (PMID: 31921036)
J Intern Med. 2012 Dec;272(6):541-61. (PMID: 23025745)
ACS Infect Dis. 2019 Jul 12;5(7):1200-1213. (PMID: 31045339)
Curr Biol. 2016 Jan 11;26(1):R14-9. (PMID: 26766224)
Antimicrob Agents Chemother. 2012 May;56(5):2696-704. (PMID: 22354291)
mBio. 2015 Jun 30;6(4):e00749. (PMID: 26126853)
Ann Clin Microbiol Antimicrob. 2007 Nov 11;6:14. (PMID: 17996119)
Future Med Chem. 2021 Mar;13(6):529-531. (PMID: 33467930)
EMBO Mol Med. 2020 Mar 6;12(3):e10264. (PMID: 32048461)
J Microbiol Biotechnol. 2017 Jun 28;27(6):1053-1064. (PMID: 28301918)
Int J Mol Sci. 2020 Nov 17;21(22):. (PMID: 33212950)
Front Microbiol. 2013 Feb 14;4:21. (PMID: 23419933)
Med Mycol. 2000 Aug;38(4):301-8. (PMID: 10975698)
Drug Deliv Transl Res. 2022 Aug;12(8):1788-1810. (PMID: 34841492)
FEBS J. 2019 Oct;286(19):3874-3891. (PMID: 31144441)
Antimicrob Agents Chemother. 1993 Aug;37(8):1614-9. (PMID: 8215272)
Nat Rev Microbiol. 2021 Apr;19(4):256-271. (PMID: 33149273)
Sci Rep. 2017 Apr 21;7(1):1048. (PMID: 28432358)
Can J Microbiol. 1980 Feb;26(2):146-54. (PMID: 6105908)
Int J Mol Sci. 2019 Nov 22;20(23):. (PMID: 31766730)
Biomolecules. 2018 Jan 19;8(1):. (PMID: 29351202)
Angew Chem Int Ed Engl. 2002 Feb 1;41(3):441-5. (PMID: 12491372)
Sci Rep. 2021 Jan 14;11(1):1267. (PMID: 33446738)
Environ Microbiol. 2012 Aug;14(8):1913-28. (PMID: 22176658)
Front Chem. 2019 Nov 28;7:824. (PMID: 31850313)
Nat Rev Microbiol. 2021 Dec;19(12):786-797. (PMID: 34183822)
PLoS Pathog. 2013;9(8):e1003526. (PMID: 23950711)
PLoS Pathog. 2011 Jan 27;7(1):e1001264. (PMID: 21298031)
PLoS One. 2022 Aug 25;17(8):e0273504. (PMID: 36006947)
Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6901-4. (PMID: 11381130)
معلومات مُعتمدة: Dor 2021; LANF_ECCELLENZA18_01 Department of Biology, University of Padova
فهرسة مساهمة: Keywords: AMP; Pseudomonas aeruginosa; antimicrobial activity; biofilm; cecropin; enantiomer; protease degradation
المشرفين على المادة: 0 (Anti-Bacterial Agents)
0 (Cecropins)
80451-05-4 (cecropin B protein, Insecta)
تواريخ الأحداث: Date Created: 20230812 Date Completed: 20230814 Latest Revision: 20230814
رمز التحديث: 20230815
مُعرف محوري في PubMed: PMC10419416
DOI: 10.3390/ijms241512496
PMID: 37569868
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms241512496