دورية أكاديمية
أعرض في EDS

Sema4D is diminished in leukocyte platelet-rich fibrin and impairs pre-osteoblastic MC3T3-E1 cells' functionality.

التفاصيل البيبلوغرافية
العنوان: Sema4D is diminished in leukocyte platelet-rich fibrin and impairs pre-osteoblastic MC3T3-E1 cells' functionality.
المؤلفون: Smanio Neto H; Faculdade São Leopoldo Mandic, Instituto de Pesquisas São Leopoldo Mandic, Implantology, Campinas, SP, Brazil., Moy PK; UCLA, Department of Oral & Maxillofacial Surgery, Los Angeles, CA, USA., Martinez EF; Faculdade São Leopoldo Mandic, Instituto de Pesquisas São Leopoldo Mandic, Oral Pathology and Cell Biology, Campinas, SP, Brazil., Pelegrine AA; Faculdade São Leopoldo Mandic, Instituto de Pesquisas São Leopoldo Mandic, Implantology, Campinas, SP, Brazil., Abdalla HB; Faculdade São Leopoldo Mandic, Instituto de Pesquisas São Leopoldo Mandic, Neuroimmune Interface of Pain Research Lab, Campinas, SP, Brazil., Clemente-Napimoga JT; Faculdade São Leopoldo Mandic, Instituto de Pesquisas São Leopoldo Mandic, Neuroimmune Interface of Pain Research Lab, Campinas, SP, Brazil., Napimoga MH; Faculdade São Leopoldo Mandic, Instituto de Pesquisas São Leopoldo Mandic, Neuroimmune Interface of Pain Research Lab, Campinas, SP, Brazil. Electronic address: marcelo.napimoga@slmandic.edu.br.
المصدر: Archives of oral biology [Arch Oral Biol] 2023 Nov; Vol. 155, pp. 105778. Date of Electronic Publication: 2023 Jul 29.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Pergamon Press Country of Publication: England NLM ID: 0116711 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1506 (Electronic) Linking ISSN: 00039969 NLM ISO Abbreviation: Arch Oral Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: Oxford : Pergamon Press
Original Publication: Oxford, New York [etc.] Pergamon Press.
مواضيع طبية MeSH: Platelet-Rich Fibrin*/metabolism , Semaphorins*/pharmacology , Semaphorins*/metabolism, Cell Differentiation/genetics ; Leukocytes/metabolism ; Osteoblasts ; Osteocalcin/metabolism ; Osteogenesis/genetics ; Animals ; Mice
مستخلص: Objective: Semaphorin 4D (Sema4D) is a coupling factor expressed on osteoclasts that may hinder osteoblast differentiation. Since the leukocyte platelet-rich fibrin (L-PRF) membrane promotes growth factor concentration, this study aims to quantify the amount of Sema4D in L-PRF membranes, and analyze the impact of Sema4D on osteoblast cell function in vitro.
Design: Enzyme-linked immunosorbent assay (ELISA) was used to quantify the levels of Sema4D in both L-PRF and whole blood (serum). To analyze the impairment of Sema4D on osteoblasts, MC3T3-E1 cells were induced to osteogenic differentiation and exposed to Sema4D ranging from 10 to 500 ng/ml concentrations. The following parameters were assayed: 1) cell viability by MTT assay after 24, 48, and 72 h; 2) matrix mineralization by Alizarin Red staining after 14 days, 3) Runt-related transcription factor 2 (RUNX-2), osteocalcin (OCN), osteonectin (ONC), bone sialoprotein (BSP) and alkaline phosphatase (ALP) gene expression by qPCR. For all data, the significance level was set at 5%.
Results: The amount of Sema4D in the whole blood (serum) was higher than in L-PRF. Osteoblasts exposed to Sema4D at all tested concentrations exhibited a decrease in matrix mineralization formation as well in RUNX-2, OCN, ONC, BSP, and ALP gene expression (p < 0.05).
Conclusion: The presence of Sema4D, a molecule known for suppressing osteoblast activity, diminishes within L-PRF, enhancing its ability to facilitate bone regeneration.
Competing Interests: Declaration of Competing Interest We wish to confirm that there are no known conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome. We confirm that the manuscript has been read and approved by all named authors and that there are no other persons who satisfied the criteria for authorship but are not listed. We further confirm that the order of authors listed in the manuscript has been approved by all of us. We confirm that we have given due consideration to the protection of intellectual property associated with this work and that there are no impediments to publication, including the timing of publication, with respect to intellectual property. In so doing we confirm that we have followed the regulations of our institutions concerning intellectual property. We further confirm that any aspect of the work covered in this manuscript that has involved either experimental animals has been conducted with the ethical approval of all relevant bodies and that such approvals are acknowledged within the manuscript.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Bone regeneration; L-PRF; Osteogenesis; Semaphorin 4D
المشرفين على المادة: 0 (CD100 antigen)
104982-03-8 (Osteocalcin)
0 (Semaphorins)
0 (Sema4d protein, mouse)
تواريخ الأحداث: Date Created: 20230812 Date Completed: 20231011 Latest Revision: 20231011
رمز التحديث: 20231215
DOI: 10.1016/j.archoralbio.2023.105778
PMID: 37572522
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-1506
DOI:10.1016/j.archoralbio.2023.105778