دورية أكاديمية
Mapping the vaginal microbiota variations in women from a community clinic in Mumbai, India.
| العنوان: | Mapping the vaginal microbiota variations in women from a community clinic in Mumbai, India. |
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| المؤلفون: | Tandon D; Department of Clinical Research, ICMR-National Institute for Research in Reproductive and Child Health, Mumbai, India. Electronic address: gdeepti167@gmail.com., Shah N; Department of Clinical Research, ICMR-National Institute for Research in Reproductive and Child Health, Mumbai, India. Electronic address: shahnr97@gmail.com., Goriwale M; Department of Clinical Research, ICMR-National Institute for Research in Reproductive and Child Health, Mumbai, India. Electronic address: mayuri.goriwale@gmail.com., Karandikar K; Department of Molecular Immunology and Microbiology, ICMR- National Institute for Research in Reproductive and Child Health (NIRRCH), Mumbai, Maharashtra, India. Electronic address: kkalyani8192@gmail.com., Begum S; Department of Biostatistics, ICMR-National Institute for Research in Reproductive and Child Health (NIRRCH), Maharashtra, India. Electronic address: begums@nirrch.res.in., Patil AD; Department of Clinical Research, ICMR-National Institute for Research in Reproductive and Child Health, Mumbai, India. Electronic address: patila@nirrch.res.in., Munne K; Department of Child Health Research, ICMR-National Institute for Research in Reproductive and Child Health, Mumbai, India. Electronic address: munnek@nirrch.res.in., Kamat S; Department of Child Health Research, ICMR-National Institute for Research in Reproductive and Child Health, Mumbai, India. Electronic address: sharmilaskamat@gmail.com., Aranha C; Department of Molecular Immunology and Microbiology, ICMR- National Institute for Research in Reproductive and Child Health (NIRRCH), Mumbai, Maharashtra, India. Electronic address: aranhac@nirrch.res.in., Bhor VM; Department of Molecular Immunology and Microbiology, ICMR- National Institute for Research in Reproductive and Child Health (NIRRCH), Mumbai, Maharashtra, India. Electronic address: bhorv@nirrch.res.in. |
| المصدر: | Indian journal of medical microbiology [Indian J Med Microbiol] 2023 Sep-Oct; Vol. 45, pp. 100393. Date of Electronic Publication: 2023 Jun 06. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 8700903 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1998-3646 (Electronic) Linking ISSN: 02550857 NLM ISO Abbreviation: Indian J Med Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York] : Elsevier Original Publication: New Delhi : Indian Association of Medical Microbiologists |
| مواضيع طبية MeSH: | Vaginosis, Bacterial*/microbiology , Microbiota*/genetics, Female ; Humans ; RNA, Ribosomal, 16S/genetics ; Vagina/microbiology |
| مستخلص: | Purpose: The vaginal microbiome contributes significantly to women's reproductive health and fluctuates due to various physiological and pathological factors. The study's objective is to map the vaginal microbiome of non-pregnant women and evaluate variations based on various potential factors influencing vaginal milieu. Methods: Fifty-two sexually active, non-pregnant women between 18 and 45 years were recruited from a community clinic and clinical history was recorded. Vaginal swabs were collected to assess the vaginal microbiome by sequencing the V3-V4 region of the 16S rRNA using the Illumina HiSeq platform, followed by data analysis with QIIME 2. Vaginal milieu was assessed by Nugent score and profiling cytokines in the cervico-vaginal lavage. Results: Lactobacillus iners (34.3%) were the most abundant species in all women. Significant changes in abundance of genera (Lactobacillus, Prevotella and Anaerococcus), expression of pro-inflammatory cytokine IFN-γ and changes in alpha and beta diversity was observed in women having asymptomatic bacterial vaginosis (BV). Differences in beta diversity were seen between healthy women and women exhibiting presence of Candida spp. Variations in the abundance of genera (Lactobacillus, Bifidobacterium, Porphyromonas) were observed in women who had delivery less than twelve months back, probably as more of these women (50%, 53.7%) had higher abnormal Nugent score. Conclusion: Lactobacillus iners was the most prevalent vaginal species in women from a Mumbai community clinic. Maximum variations in the vaginal microbiome characterized by a perturbation of the Lactobacillus predominant vaginal microbiota are seen in those women who have asymptomatic BV and childbirth within last twelve months. (Copyright © 2023 Indian Association of Medical Microbiologists. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: 16S rRNA gene sequencing; Asymptomatic vaginal infections; Bacterial vaginosis; Cytokines; Time after delivery; Vaginal microbiome |
| المشرفين على المادة: | 0 (RNA, Ribosomal, 16S) |
| SCR Organism: | Lactobacillus iners |
| تواريخ الأحداث: | Date Created: 20230812 Date Completed: 20231102 Latest Revision: 20231104 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.ijmmb.2023.100393 |
| PMID: | 37573043 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 1998-3646 |
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| DOI: | 10.1016/j.ijmmb.2023.100393 |