دورية أكاديمية

Dual host-pathogen small RNA sequencing during wheat stem rust infection.

التفاصيل البيبلوغرافية
العنوان: Dual host-pathogen small RNA sequencing during wheat stem rust infection.
المؤلفون: Mueth NA; Molecular Plant Sciences Program, Washington State University, Pullman, Washington, USA. mueth@uw.edu.; Department of Plant Pathology, Washington State University, Pullman, Washington, USA. mueth@uw.edu.; Genome Sciences Department, University of Washington, Seattle, WA, USA. mueth@uw.edu., Hulbert SH; Molecular Plant Sciences Program, Washington State University, Pullman, Washington, USA.; Department of Plant Pathology, Washington State University, Pullman, Washington, USA.
المصدر: BMC research notes [BMC Res Notes] 2023 Aug 14; Vol. 16 (1), pp. 171. Date of Electronic Publication: 2023 Aug 14.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Biomed Central Country of Publication: England NLM ID: 101462768 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-0500 (Electronic) Linking ISSN: 17560500 NLM ISO Abbreviation: BMC Res Notes Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Biomed Central, 2008.
مواضيع طبية MeSH: MicroRNAs*/genetics , Basidiomycota*/genetics, Sequence Analysis, RNA ; Base Sequence ; Gene Library ; Plant Diseases/genetics ; Plant Diseases/microbiology
مستخلص: Objectives: RNA sequencing of two organisms in a symbiotic interaction can yield insights that are not found in samples from each organism alone. We present a sequencing dataset focusing on the small RNA fraction from wheat plants (Triticum aestivum) infected with the biotrophic pathogen wheat stem rust fungus (Puccinia graminis f.sp. tritici). Simultaneous small RNA sequencing of this agronomically important crop and its adversary can lead to a better understanding of the role of noncoding RNAs in both plant and fungal biology.
Data Description: Small RNA libraries were constructed from infected and mock-infected plant tissue and sequenced on the Ion Torrent platform. Quality control was performed to ensure sample and data integrity. Using this dataset, researchers can employ previously established methods to map subsets of reads exclusively to each organism's genome. Subsequent analyses can be undertaken to discover microRNAs, predict small RNA targets, and generate hypotheses for further laboratory experiments.
(© 2023. BioMed Central Ltd., part of Springer Nature.)
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معلومات مُعتمدة: 2017-67011-26066 National Institute of Food and Agriculture; 2017-67011-26066 National Institute of Food and Agriculture; Mistletoe Research Fellowship Momental Foundation
فهرسة مساهمة: Keywords: Fungi; Plant pathogen; Puccinia; Small RNA; Stem rust; Wheat
المشرفين على المادة: 0 (MicroRNAs)
تواريخ الأحداث: Date Created: 20230814 Date Completed: 20230816 Latest Revision: 20231121
رمز التحديث: 20231121
مُعرف محوري في PubMed: PMC10426046
DOI: 10.1186/s13104-023-06426-8
PMID: 37580827
قاعدة البيانات: MEDLINE
الوصف
تدمد:1756-0500
DOI:10.1186/s13104-023-06426-8