التفاصيل البيبلوغرافية
| العنوان: |
[Chloroplast genomic characterization and phylogenetic analysis of Pellionia scabra ]. |
| المؤلفون: |
Yan L; Department of Horticulture, College of Agriculture, Guizhou University, Guiyang 550025, Guizhou, China., Yang X; Department of Horticulture, College of Agriculture, Guizhou University, Guiyang 550025, Guizhou, China., Wu Y; Department of Horticulture, College of Agriculture, Guizhou University, Guiyang 550025, Guizhou, China., Wang X; Department of Horticulture, College of Agriculture, Guizhou University, Guiyang 550025, Guizhou, China., Hu X; Department of Horticulture, College of Agriculture, Guizhou University, Guiyang 550025, Guizhou, China. |
| المصدر: |
Sheng wu gong cheng xue bao = Chinese journal of biotechnology [Sheng Wu Gong Cheng Xue Bao] 2023 Jul 25; Vol. 39 (7), pp. 2914-2925. |
| نوع المنشور: |
English Abstract; Journal Article |
| اللغة: |
Chinese |
| بيانات الدورية: |
Publisher: Ke xue chu ban she Country of Publication: China NLM ID: 9426463 Publication Model: Print Cited Medium: Internet ISSN: 1872-2075 (Electronic) Linking ISSN: 10003061 NLM ISO Abbreviation: Sheng Wu Gong Cheng Xue Bao Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Beijing : Ke xue chu ban she, |
| مواضيع طبية MeSH: |
Genome, Chloroplast*/genetics , Urticaceae*/genetics, Phylogeny ; Genomics ; Chloroplasts/genetics ; Codon |
| مستخلص: |
Pellionia scabra belongs to the genus Pellionia in the family of Urticaceae , and is a high-quality wild vegetables with high nutritional value. In this study, high-throughput techniques were used to sequence, assemble and annotate the chloroplast genome. We also analyzed its structure, and construct the phylogenetic trees from the P . scabra to further study the chloroplast genome characteristics. The results showed that the chloroplast genome size was 153 220 bp, and the GC content was 36.4%, which belonged to the typical tetrad structure in P . scabra . The chloroplast genome encodes 130 genes, including 85 protein-coding genes, 37 tRNA genes, and 8 rRNA genes in P . scabra . Among them, 15 genes contained 1 intron, 2 genes contained 2 introns, and rps12 had trans-splicing, respectively. In P . scabra , chloroplast genomes could be divided into four categories, including 43 photosynthesis, 64 self-replication, other 7 coding proteins, and 4 unknown functions. A total of 51 073 codons were detected in the chloroplast genome, among which the codon encoding leucine (Leu) accounted for the largest proportion, and the codon preferred to use A and U bases. There were 72 simple sequence repeats (SSRs) in the chloroplast genome of P . scabra , containing 58 single nucleotides, 12 dinucleotides, 1 trinucleotide, and 1 tetranucleotide. The ycf 1 gene expansion was present at the IRb/SSC boundary. The phylogenetic trees showed that P . scabra (OL800583) was most closely related to Elatostema stewardii (MZ292972), Elatostema dissectum (MK227819) and Elatostema laevissimum var. laevissimum (MN189961). Taken together, our results provide worthwhile information for understanding the identification, genetic evolution, and genomics research of P . scabra species. |
| فهرسة مساهمة: |
Keywords: Pellionia scabra; boundary analysis of IR region; chloroplast genome; codon preference; phylogeny; simple sequence repeat |
| المشرفين على المادة: |
0 (Codon) |
| تواريخ الأحداث: |
Date Created: 20230816 Date Completed: 20230817 Latest Revision: 20230817 |
| رمز التحديث: |
20231215 |
| DOI: |
10.13345/j.cjb.220830 |
| PMID: |
37584139 |
| قاعدة البيانات: |
MEDLINE |
