دورية أكاديمية
Hepatobiliary organoids derived from leporids support the replication of hepatotropic lagoviruses.
| العنوان: | Hepatobiliary organoids derived from leporids support the replication of hepatotropic lagoviruses. |
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| المؤلفون: | Kardia E; Health and Biosecurity Commonwealth Scientific and Industrial Research Organisation, Acton, ACT 2601, Australia., Fakhri O; Health and Biosecurity Commonwealth Scientific and Industrial Research Organisation, Acton, ACT 2601, Australia., Pavy M; Health and Biosecurity Commonwealth Scientific and Industrial Research Organisation, Acton, ACT 2601, Australia., Mason H; Health and Biosecurity Commonwealth Scientific and Industrial Research Organisation, Acton, ACT 2601, Australia., Huang N; Health and Biosecurity Commonwealth Scientific and Industrial Research Organisation, Acton, ACT 2601, Australia., Smertina E; Health and Biosecurity Commonwealth Scientific and Industrial Research Organisation, Acton, ACT 2601, Australia.; Faculty of Science and Technology, University of Canberra, Bruce, ACT 2617, Australia.; Centre for Invasive Species Solutions, Bruce, ACT 2617, Australia., Jenckel M; Health and Biosecurity Commonwealth Scientific and Industrial Research Organisation, Acton, ACT 2601, Australia., Peng NYG; Health and Biosecurity Commonwealth Scientific and Industrial Research Organisation, Acton, ACT 2601, Australia., Estes MK; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA.; Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA., Strive T; Health and Biosecurity Commonwealth Scientific and Industrial Research Organisation, Acton, ACT 2601, Australia.; Centre for Invasive Species Solutions, Bruce, ACT 2617, Australia., Frese M; Health and Biosecurity Commonwealth Scientific and Industrial Research Organisation, Acton, ACT 2601, Australia.; Faculty of Science and Technology, University of Canberra, Bruce, ACT 2617, Australia., Smith I; Health and Biosecurity Commonwealth Scientific and Industrial Research Organisation, Acton, ACT 2601, Australia., Hall RN; Health and Biosecurity Commonwealth Scientific and Industrial Research Organisation, Acton, ACT 2601, Australia.; Centre for Invasive Species Solutions, Bruce, ACT 2617, Australia.; Present address: Ausvet, Bruce, ACT 2617, Australia. |
| المصدر: | The Journal of general virology [J Gen Virol] 2023 Aug; Vol. 104 (8). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Microbiology Society Country of Publication: England NLM ID: 0077340 Publication Model: Print Cited Medium: Internet ISSN: 1465-2099 (Electronic) Linking ISSN: 00221317 NLM ISO Abbreviation: J Gen Virol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : London : Microbiology Society Original Publication: London, Cambridge Univ. Press for the Society for General Microbiology. |
| مواضيع طبية MeSH: | Caliciviridae Infections* , Hemorrhagic Disease Virus, Rabbit*/genetics , Lagovirus*/genetics , Hares*, Animals ; Cats ; Mice ; Rabbits ; Phylogeny ; Organoids |
| مستخلص: | The genus Lagovirus of the family Caliciviridae contains some of the most virulent vertebrate viruses known. Lagoviruses infect leporids, such as rabbits, hares and cottontails. Highly pathogenic viruses such as Rabbit haemorrhagic disease virus 1 (RHDV1) cause a fulminant hepatitis that typically leads to disseminated intravascular coagulation within 24-72 h of infection, killing over 95 % of susceptible animals. Research into the pathophysiological mechanisms that are responsible for this extreme phenotype has been hampered by the lack of a reliable culture system. Here, we report on a new ex vivo model for the cultivation of lagoviruses in cells derived from the European rabbit ( Oryctolagus cuniculus ) and European brown hare ( Lepus europaeus ). We show that three different lagoviruses, RHDV1, RHDV2 and RHDVa-K5, replicate in monolayer cultures derived from rabbit hepatobiliary organoids, but not in monolayer cultures derived from cat ( Felis catus ) or mouse ( Mus musculus ) organoids. Virus multiplication was demonstrated by (i) an increase in viral RNA levels, (ii) the accumulation of dsRNA viral replication intermediates and (iii) the expression of viral structural and non-structural proteins. The establishment of an organoid culture system for lagoviruses will facilitate studies with considerable implications for the conservation of endangered leporid species in Europe and North America, and the biocontrol of overabundant rabbit populations in Australia and New Zealand. |
| فهرسة مساهمة: | Keywords: Oryctolagus cuniculus; RHDV; cell culture model; ex vivo; lagovirus; organoids; rabbit |
| تواريخ الأحداث: | Date Created: 20230816 Date Completed: 20230817 Latest Revision: 20230817 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1099/jgv.0.001874 |
| PMID: | 37584657 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1465-2099 |
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| DOI: | 10.1099/jgv.0.001874 |