دورية أكاديمية
Platelet factor XIII-A regulates platelet function and promotes clot retraction and stability.
العنوان: | Platelet factor XIII-A regulates platelet function and promotes clot retraction and stability. |
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المؤلفون: | Mitchell JL; Institute for Cardiovascular Research, University of Birmingham, Birmingham, UK., Little G; Institute for Cardiovascular and Metabolic Research, School of Biological Sciences, University of Reading, Reading, UK., Bye AP; School of Pharmacy, University of Reading, Reading, UK., Gaspar RS; Heart Institute, University of Sao Paulo School of Medicine, Sao Paulo, Brazil., Unsworth AJ; Department of Life Sciences, Faculty of Science and Engineering, Manchester Metropolitan University, Manchester, UK., Kriek N; Institute for Cardiovascular and Metabolic Research, School of Biological Sciences, University of Reading, Reading, UK., Sage T; Institute for Cardiovascular and Metabolic Research, School of Biological Sciences, University of Reading, Reading, UK., Stainer A; Institute for Cardiovascular and Metabolic Research, School of Biological Sciences, University of Reading, Reading, UK., Sangowawa I; Institute for Cardiovascular and Metabolic Research, School of Biological Sciences, University of Reading, Reading, UK., Morrow GB; Oxford University Hospitals NHS Foundation Trust, Blood Theme Oxford Biomedical Research Centre, Oxford, UK.; Radcliffe Department of Medicine, University of Oxford, Oxford, UK., Bastos RN; Oxford Nanoimaging, Oxford, UK., Shapiro S; Oxford University Hospitals NHS Foundation Trust, Blood Theme Oxford Biomedical Research Centre, Oxford, UK.; Radcliffe Department of Medicine, University of Oxford, Oxford, UK., Desborough MJR; Oxford University Hospitals NHS Foundation Trust, Blood Theme Oxford Biomedical Research Centre, Oxford, UK., Curry N; Oxford University Hospitals NHS Foundation Trust, Blood Theme Oxford Biomedical Research Centre, Oxford, UK.; Radcliffe Department of Medicine, University of Oxford, Oxford, UK., Gibbins JM; Institute for Cardiovascular and Metabolic Research, School of Biological Sciences, University of Reading, Reading, UK., Whyte CS; Aberdeen Cardiovascular & Diabetes Centre, School of Medicine, Medical Sciences and Nutrition, Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK., Mutch NJ; Aberdeen Cardiovascular & Diabetes Centre, School of Medicine, Medical Sciences and Nutrition, Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK., Jones CI; Institute for Cardiovascular and Metabolic Research, School of Biological Sciences, University of Reading, Reading, UK. |
المصدر: | Research and practice in thrombosis and haemostasis [Res Pract Thromb Haemost] 2023 Jun 07; Vol. 7 (5), pp. 100200. Date of Electronic Publication: 2023 Jun 07 (Print Publication: 2023). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 101703775 Publication Model: eCollection Cited Medium: Internet ISSN: 2475-0379 (Electronic) Linking ISSN: 24750379 NLM ISO Abbreviation: Res Pract Thromb Haemost Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Publication: 2023- : [New York] : Elsevier Original Publication: Hoboken, NJ : John Wiley & Sons, Inc., [2017]- |
مستخلص: | Background: Factor XIII (FXIII) is an important proenzyme in the hemostatic system. The plasma-derived enzyme activated FXIII cross-links fibrin fibers within thrombi to increase their mechanical strength and cross-links fibrin to fibrinolytic inhibitors, specifically α Objectives: This study aims to identify the role of platelet FXIII-A in platelet function. Methods: We used normal healthy platelets with a cell permeable FXIII inhibitor and platelets from FXIII-deficient patients as a FXIII-free platelet model in a range of platelet function and clotting tests. Results: Our data demonstrate that platelet FXIII-A enhances fibrinogen binding to the platelet surface upon agonist stimulation and improves the binding of platelets to fibrinogen and aggregation under flow in a whole-blood thrombus formation assay. In the absence of FXIII-A, platelets show reduced sensitivity to agonist stimulation, including decreased P-selectin exposure and fibrinogen binding. We show that FXIII-A is involved in platelet spreading where a lack of FXIII-A reduces the ability of platelets to fully spread on fibrinogen and collagen. Our data demonstrate that platelet FXIII-A is important for clot retraction where clots formed in its absence retracted to a lesser extent. Conclusion: Overall, this study shows that platelet FXIII-A functions during thrombus formation by aiding platelet activation and thrombus retraction in addition to its antifibrinolytic roles. (© 2023 The Authors.) |
References: | J Clin Invest. 1983 May;71(5):1336-41. (PMID: 6853717) Trans Assoc Am Physicians. 1991;104:21-8. (PMID: 1845147) Blood. 2013 Nov 7;122(19):3340-8. (PMID: 24002447) Blood. 2005 May 15;105(10):3879-87. (PMID: 15671445) Arterioscler Thromb Vasc Biol. 2012 Dec;32(12):2938-45. (PMID: 23087356) Thromb Res. 1983 May 1;30(3):273-88. (PMID: 6223406) J Thromb Haemost. 2011 Apr;9(4):820-33. (PMID: 21320283) Curr Opin Cell Biol. 1992 Oct;4(5):766-71. (PMID: 1419055) J Thromb Haemost. 2008 Nov;6(11):1944-52. (PMID: 18761725) J Cell Biol. 1992 Sep;118(6):1421-42. (PMID: 1325975) J Thromb Haemost. 2010 Sep;8(9):2017-24. (PMID: 20586921) J Thromb Haemost. 2023 Aug;21(8):2248-2259. (PMID: 37085037) Science. 1968 May 24;160(3830):892-3. (PMID: 4967475) Blood. 2017 Nov 16;130(20):2171-2179. (PMID: 28972013) Thromb Haemost. 1984 Jul 29;51(3):388-91. (PMID: 6388014) J Cell Biol. 1999 Feb 22;144(4):745-54. (PMID: 10037795) J Biol Chem. 2004 Jul 16;279(29):30697-706. (PMID: 15131115) Thromb Haemost. 2009 Jul;102(1):83-9. (PMID: 19572072) Arch Biochem Biophys. 1979 Jan;192(1):100-11. (PMID: 434811) Blood. 2010 Feb 11;115(6):1277-9. (PMID: 19996413) Nat Commun. 2015 Jun 01;6:7254. (PMID: 26028144) J Biol Chem. 1971 Sep 25;246(18):5851-4. (PMID: 5096097) Blood Adv. 2021 Oct 26;5(20):4017-4030. (PMID: 34474473) Thromb Haemost. 1993 Mar 1;69(3):282-5. (PMID: 8097064) Haematologica. 2016 Apr;101(4):427-36. (PMID: 26721892) Circulation. 2012 May 22;125(20):2479-91. (PMID: 22528526) Blood. 2014 Dec 18;124(26):3982-90. (PMID: 25331118) J Biol Chem. 1995 May 12;270(19):11358-66. (PMID: 7744773) Schweiz Med Wochenschr. 1957 Oct 2;87(39-40):1220-1. (PMID: 13485983) Thromb Haemost. 2002 Aug;88(2):315-20. (PMID: 12195706) Biophys Chem. 1974 Aug;2(2):164-9. (PMID: 4279707) Thromb Haemost. 2019 Jun;119(6):906-915. (PMID: 30934104) Biochim Biophys Acta. 1980 Mar 20;628(3):365-75. (PMID: 6892787) FEBS J. 2020 Feb;287(3):452-464. (PMID: 31407850) Nature. 2002 Jan 10;415(6868):175-9. (PMID: 11805836) Thromb Res. 1976 Apr;8(4):453-65. (PMID: 1273806) Blood. 2015 Apr 16;125(16):2568-78. (PMID: 25712989) Blood. 2011 Jun 9;117(23):6371-4. (PMID: 21471521) J Cell Biol. 2007 Nov 5;179(3):553-65. (PMID: 17967945) Am J Physiol. 1966 Dec;211(6):1472-6. (PMID: 4959531) J Clin Invest. 2014 Aug;124(8):3590-600. (PMID: 24983320) Pol Tyg Lek (Wars). 1955 Feb 7;10(6):191. (PMID: 14371128) Semin Thromb Hemost. 2017 Mar;43(2):115-128. (PMID: 28215042) J Cell Sci. 2004 Jul 15;117(Pt 16):3415-25. (PMID: 15252124) Blood. 2012 Jan 26;119(4):1054-63. (PMID: 22045984) Blood Adv. 2018 Jan 03;2(1):25-35. (PMID: 29344582) J Cell Physiol. 2018 Sep;233(9):7497-7513. (PMID: 29663380) J Clin Invest. 1980 Feb;65(2):290-7. (PMID: 6444305) |
معلومات مُعتمدة: | MR/T024054/1 United Kingdom MRC_ Medical Research Council |
فهرسة مساهمة: | Keywords: clot retraction; factor XIII; fibrinogen; fibrinolysis; platelet; platelet activation |
تواريخ الأحداث: | Date Created: 20230821 Latest Revision: 20240214 |
رمز التحديث: | 20240214 |
مُعرف محوري في PubMed: | PMC10439398 |
DOI: | 10.1016/j.rpth.2023.100200 |
PMID: | 37601014 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2475-0379 |
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DOI: | 10.1016/j.rpth.2023.100200 |