دورية أكاديمية
TRPV2, a novel player in the human ovary and human granulosa cells.
| العنوان: | TRPV2, a novel player in the human ovary and human granulosa cells. |
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| المؤلفون: | Eubler K; Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Faculty of Medicine, Ludwig-Maximilian-University (LMU) Munich, Planegg-Martinsried, Germany., Caban KM; Laboratory for Functional Genome Analysis LAFUGA, Gene Center, LMU Munich, Munich, Germany., Dissen GA; Molecular Virology Core, Oregon Health & Science University (OHSU), Oregon National Primate Research Center, Beaverton, OR, USA., Berg U; A.R.T. Bogenhausen, Munich, Germany., Berg D; A.R.T. Bogenhausen, Munich, Germany., Herrmann C; Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Faculty of Medicine, Ludwig-Maximilian-University (LMU) Munich, Planegg-Martinsried, Germany., Kreitmair N; Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Faculty of Medicine, Ludwig-Maximilian-University (LMU) Munich, Planegg-Martinsried, Germany., Tiefenbacher A; Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Faculty of Medicine, Ludwig-Maximilian-University (LMU) Munich, Planegg-Martinsried, Germany., Fröhlich T; Laboratory for Functional Genome Analysis LAFUGA, Gene Center, LMU Munich, Munich, Germany., Mayerhofer A; Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Faculty of Medicine, Ludwig-Maximilian-University (LMU) Munich, Planegg-Martinsried, Germany. |
| المصدر: | Molecular human reproduction [Mol Hum Reprod] 2023 Aug 30; Vol. 29 (9). |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press for the European Society for Human Reproduction and Embryology Country of Publication: England NLM ID: 9513710 Publication Model: Print Cited Medium: Internet ISSN: 1460-2407 (Electronic) Linking ISSN: 13609947 NLM ISO Abbreviation: Mol Hum Reprod Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Oxford University Press for the European Society for Human Reproduction and Embryology Original Publication: Oxford, UK : Published for the European Society for Human Reproduction and Embryology by Oxford University Press, [1995- |
| مواضيع طبية MeSH: | Ovary* , Cannabidiol*/pharmacology, Female ; Humans ; Proteomics ; Granulosa Cells ; Corpus Luteum ; TRPV Cation Channels/genetics |
| مستخلص: | The cation channel 'transient receptor potential vanilloid 2' (TRPV2) is activated by a broad spectrum of stimuli, including mechanical stretch, endogenous and exogenous chemical compounds, hormones, growth factors, reactive oxygen species, and cannabinoids. TRPV2 is known to be involved in inflammatory and immunological processes, which are also of relevance in the ovary. Yet, neither the presence nor possible roles of TRPV2 in the ovary have been investigated. Data mining indicated expression, for example, in granulosa cells (GCs) of the human ovary in situ, which was retained in cultured GCs derived from patients undergoing medical reproductive procedures. We performed immunohistochemistry of human and rhesus monkey ovarian sections and then cellular studies in cultured GCs, employing the preferential TRPV2 agonist cannabidiol (CBD). Immunohistochemistry showed TRPV2 staining in GCs of large antral follicles and corpus luteum but also in theca, endothelial, and stromal cells. TRPV2 transcript and protein levels increased upon administration of hCG or forskolin. Acutely, application of the agonist CBD elicited transient Ca2+ fluxes, which was followed by the production and secretion of several inflammatory factors, especially COX2, IL6, IL8, and PTX3, in a time- and dose-dependent manner. CBD interfered with progesterone synthesis and altered both the proteome and secretome, as revealed by a proteomic study. While studies are somewhat hampered by the lack of highly specific TRPV2 agonist or antagonists, the results pinpoint TRPV2 as a modulator of inflammation with possible roles in human ovarian (patho-)physiology. Finally, as TRPV2 is activated by cannabinoids, their possible ovarian actions should be further evaluated. (© The Author(s) 2023. Published by Oxford University Press on behalf of European Society of Human Reproduction and Embryology. All rights reserved. For permissions, please email: journals.permissions@oup.com.) |
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| معلومات مُعتمدة: | P51 OD011092 United States OD NIH HHS |
| فهرسة مساهمة: | Keywords: calcium; cannabidiol; corpus luteum; female gonad; follicle; inflammation; ion channel |
| المشرفين على المادة: | 19GBJ60SN5 (Cannabidiol) 0 (TRPV2 protein, human) 0 (TRPV Cation Channels) |
| تواريخ الأحداث: | Date Created: 20230823 Date Completed: 20230912 Latest Revision: 20240824 |
| رمز التحديث: | 20240826 |
| مُعرف محوري في PubMed: | PMC10493183 |
| DOI: | 10.1093/molehr/gaad029 |
| PMID: | 37610352 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1460-2407 |
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| DOI: | 10.1093/molehr/gaad029 |