دورية أكاديمية
TRPV2, a novel player in the human ovary and human granulosa cells.
العنوان: | TRPV2, a novel player in the human ovary and human granulosa cells. |
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المؤلفون: | Eubler K; Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Faculty of Medicine, Ludwig-Maximilian-University (LMU) Munich, Planegg-Martinsried, Germany., Caban KM; Laboratory for Functional Genome Analysis LAFUGA, Gene Center, LMU Munich, Munich, Germany., Dissen GA; Molecular Virology Core, Oregon Health & Science University (OHSU), Oregon National Primate Research Center, Beaverton, OR, USA., Berg U; A.R.T. Bogenhausen, Munich, Germany., Berg D; A.R.T. Bogenhausen, Munich, Germany., Herrmann C; Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Faculty of Medicine, Ludwig-Maximilian-University (LMU) Munich, Planegg-Martinsried, Germany., Kreitmair N; Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Faculty of Medicine, Ludwig-Maximilian-University (LMU) Munich, Planegg-Martinsried, Germany., Tiefenbacher A; Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Faculty of Medicine, Ludwig-Maximilian-University (LMU) Munich, Planegg-Martinsried, Germany., Fröhlich T; Laboratory for Functional Genome Analysis LAFUGA, Gene Center, LMU Munich, Munich, Germany., Mayerhofer A; Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Faculty of Medicine, Ludwig-Maximilian-University (LMU) Munich, Planegg-Martinsried, Germany. |
المصدر: | Molecular human reproduction [Mol Hum Reprod] 2023 Aug 30; Vol. 29 (9). |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Oxford University Press for the European Society for Human Reproduction and Embryology Country of Publication: England NLM ID: 9513710 Publication Model: Print Cited Medium: Internet ISSN: 1460-2407 (Electronic) Linking ISSN: 13609947 NLM ISO Abbreviation: Mol Hum Reprod Subsets: MEDLINE |
أسماء مطبوعة: | Publication: Oxford : Oxford University Press for the European Society for Human Reproduction and Embryology Original Publication: Oxford, UK : Published for the European Society for Human Reproduction and Embryology by Oxford University Press, [1995- |
مواضيع طبية MeSH: | Ovary* , Cannabidiol*/pharmacology, Female ; Humans ; Proteomics ; Granulosa Cells ; Corpus Luteum ; TRPV Cation Channels/genetics |
مستخلص: | The cation channel 'transient receptor potential vanilloid 2' (TRPV2) is activated by a broad spectrum of stimuli, including mechanical stretch, endogenous and exogenous chemical compounds, hormones, growth factors, reactive oxygen species, and cannabinoids. TRPV2 is known to be involved in inflammatory and immunological processes, which are also of relevance in the ovary. Yet, neither the presence nor possible roles of TRPV2 in the ovary have been investigated. Data mining indicated expression, for example, in granulosa cells (GCs) of the human ovary in situ, which was retained in cultured GCs derived from patients undergoing medical reproductive procedures. We performed immunohistochemistry of human and rhesus monkey ovarian sections and then cellular studies in cultured GCs, employing the preferential TRPV2 agonist cannabidiol (CBD). Immunohistochemistry showed TRPV2 staining in GCs of large antral follicles and corpus luteum but also in theca, endothelial, and stromal cells. TRPV2 transcript and protein levels increased upon administration of hCG or forskolin. Acutely, application of the agonist CBD elicited transient Ca2+ fluxes, which was followed by the production and secretion of several inflammatory factors, especially COX2, IL6, IL8, and PTX3, in a time- and dose-dependent manner. CBD interfered with progesterone synthesis and altered both the proteome and secretome, as revealed by a proteomic study. While studies are somewhat hampered by the lack of highly specific TRPV2 agonist or antagonists, the results pinpoint TRPV2 as a modulator of inflammation with possible roles in human ovarian (patho-)physiology. Finally, as TRPV2 is activated by cannabinoids, their possible ovarian actions should be further evaluated. (© The Author(s) 2023. Published by Oxford University Press on behalf of European Society of Human Reproduction and Embryology. All rights reserved. For permissions, please email: journals.permissions@oup.com.) |
References: | Br J Pharmacol. 2011 Aug;163(7):1479-94. (PMID: 21175579) Mol Hum Reprod. 1996 Apr;2(4):245-50. (PMID: 9238687) Trends Endocrinol Metab. 2008 Aug;19(6):191-6. (PMID: 18407514) Int J Mol Sci. 2021 Apr 29;22(9):. (PMID: 33946947) J Clin Endocrinol Metab. 2016 Sep;101(9):3459-68. (PMID: 27228368) Nat Immunol. 2010 Mar;11(3):232-9. (PMID: 20118928) J Clin Endocrinol Metab. 1994 Aug;79(2):633-42. (PMID: 7519193) Int J Mol Sci. 2018 Sep 19;19(9):. (PMID: 30235802) Lab Invest. 2020 Feb;100(2):186-198. (PMID: 31653969) Am J Reprod Immunol. 2020 Aug;84(2):e13265. (PMID: 32395847) Dis Markers. 2020 Dec 10;2020:8892312. (PMID: 33376561) PLoS One. 2009;4(2):e4579. (PMID: 19238202) Biochim Biophys Acta. 2009 Mar;1793(3):528-39. (PMID: 19321128) Biol Reprod. 1980 Feb;22(1):73-106. (PMID: 6991013) Hum Reprod. 1999 Jan;14(1):162-6. (PMID: 10374114) Cancer Res. 2010 Feb 1;70(3):1225-35. (PMID: 20103638) Cell Biol Int. 2010 Apr 08;34(5):531-6. (PMID: 20156197) Fertil Steril. 1992 Nov;58(5):934-40. (PMID: 1426379) J Clin Endocrinol Metab. 2004 Sep;89(9):4503-12. (PMID: 15356055) Physiol Res. 2012;61(1):113-24. (PMID: 21574765) Mol Cell. 2018 Dec 20;72(6):1021-1034.e4. (PMID: 30472193) Histochem Cell Biol. 2021 Nov;156(5):509-517. (PMID: 34313845) Reproduction. 2001 Feb;121(2):307-14. (PMID: 11226055) Cell Death Dis. 2015 Mar 12;6:e1685. (PMID: 25766324) Neurotherapeutics. 2015 Oct;12(4):699-730. (PMID: 26264914) Fertil Steril. 1992 Jun;57(6):1230-4. (PMID: 1601144) J Biomed Sci. 2016 Mar 29;23:36. (PMID: 27026099) Front Cell Neurosci. 2014 Aug 01;8:195. (PMID: 25136293) Cell Host Microbe. 2011 Sep 15;10(3):248-59. (PMID: 21925112) Biol Reprod. 1994 Feb;50(2):233-8. (PMID: 8142541) Neural Regen Res. 2022 Dec;17(12):2693-2694. (PMID: 35662213) Reprod Sci. 2016 Dec;23(12):1656-1661. (PMID: 27821562) Endocrinology. 1996 Sep;137(9):3762-9. (PMID: 8756544) FEBS J. 2013 Nov;280(21):5471-87. (PMID: 23615321) Semin Reprod Med. 2015 Jul;33(4):270-5. (PMID: 26132931) J Reprod Immunol. 1997 Feb;32(3):201-19. (PMID: 9080384) Biol Reprod. 2017 Mar 1;96(3):652-663. (PMID: 28339599) Biol Reprod. 2022 Feb 22;106(2):351-365. (PMID: 34982142) J Cell Physiol. 2007 Mar;210(3):692-702. (PMID: 17154364) Fertil Steril. 2009 Jan;91(1):231-9. (PMID: 18829011) Hum Reprod Update. 2023 Jan 5;29(1):1-23. (PMID: 35856663) Biol Reprod. 2021 Jun 4;104(6):1205-1217. (PMID: 33739372) Exp Mol Med. 2022 May;54(5):553-566. (PMID: 35501376) Sci Rep. 2019 Mar 5;9(1):3585. (PMID: 30837663) Endocr Rev. 2019 Apr 1;40(2):369-416. (PMID: 30496379) Biol Reprod. 2003 May;68(5):1895-902. (PMID: 12606366) J Biol Chem. 2007 May 25;282(21):15894-902. (PMID: 17395593) J Biol Chem. 1990 Sep 5;265(25):15267-74. (PMID: 2203773) Hum Reprod. 2021 Jun 18;36(7):1922-1931. (PMID: 33954787) Front Immunol. 2021 Nov 05;12:739918. (PMID: 34804020) J Ovarian Res. 2019 Jan 15;12(1):3. (PMID: 30646937) Hum Reprod. 1993 Oct;8(10):1762-75. (PMID: 8300842) Chem Phys Lipids. 2002 Dec 31;121(1-2):211-27. (PMID: 12505702) Front Oncol. 2022 Jan 07;11:744257. (PMID: 35070963) J Neurosci. 2010 Mar 31;30(13):4601-12. (PMID: 20357111) J Neurosci. 2008 Jun 11;28(24):6231-8. (PMID: 18550765) Nucleic Acids Res. 2001 May 1;29(9):e45. (PMID: 11328886) Front Endocrinol (Lausanne). 2019 Jul 09;10:452. (PMID: 31338068) Cancers (Basel). 2020 Jun 04;12(6):. (PMID: 32512900) Nat Commun. 2020 Aug 12;11(1):4038. (PMID: 32788587) Cell Death Discov. 2019 Feb 11;5:67. (PMID: 30774995) Prog Lipid Res. 2009 Nov;48(6):344-54. (PMID: 19602425) J Med Chem. 2019 Feb 14;62(3):1373-1384. (PMID: 30620187) Biochem Biophys Rep. 2021 Nov 18;28:101173. (PMID: 34841092) Handb Exp Pharmacol. 2014;222:247-72. (PMID: 24756709) Nature. 1999 Apr 1;398(6726):436-41. (PMID: 10201375) Front Physiol. 2022 Jun 13;13:859790. (PMID: 35770187) Obstet Gynecol. 1996 Apr;87(4):581-7. (PMID: 8602312) Mol Reprod Dev. 2014 Apr;81(4):284-314. (PMID: 24273059) Hum Reprod. 1995 Aug;10(8):2198-203. (PMID: 8567873) Front Mol Neurosci. 2019 Jan 15;11:487. (PMID: 30697147) Front Immunol. 2013 Feb 14;4:34. (PMID: 23420671) Nucleic Acids Res. 2022 Jan 7;50(D1):D543-D552. (PMID: 34723319) Mol Endocrinol. 2008 May;22(5):1260-73. (PMID: 18258683) J Clin Endocrinol Metab. 2021 Jan 23;106(2):e721-e738. (PMID: 33247906) Nat Cell Biol. 1999 Jul;1(3):165-70. (PMID: 10559903) Oncotarget. 2016 May 27;9(71):33459-33470. (PMID: 30323891) Elife. 2019 Sep 30;8:. (PMID: 31566564) Nat Methods. 2016 Sep;13(9):731-40. (PMID: 27348712) Front Cell Dev Biol. 2022 Dec 02;10:1038107. (PMID: 36531957) Am J Physiol. 1983 Feb;244(2):E177-85. (PMID: 6297308) J Biol Chem. 2008 Jun 6;283(23):16124-34. (PMID: 18390906) Gynecol Obstet Invest. 2012;73(3):189-94. (PMID: 22414682) Front Genet. 2020 Apr 30;11:396. (PMID: 32425983) Biochem Biophys Res Commun. 2010 Jul 23;398(2):284-9. (PMID: 20599720) Hum Reprod. 2016 Feb;31(2):436-44. (PMID: 26740577) J Physiol Sci. 2016 Sep;66(5):359-65. (PMID: 26841959) Ann Surg Oncol. 2022 May;29(5):2944-2956. (PMID: 34855064) |
معلومات مُعتمدة: | P51 OD011092 United States OD NIH HHS |
فهرسة مساهمة: | Keywords: calcium; cannabidiol; corpus luteum; female gonad; follicle; inflammation; ion channel |
المشرفين على المادة: | 19GBJ60SN5 (Cannabidiol) 0 (TRPV2 protein, human) 0 (TRPV Cation Channels) |
تواريخ الأحداث: | Date Created: 20230823 Date Completed: 20230912 Latest Revision: 20240824 |
رمز التحديث: | 20240826 |
مُعرف محوري في PubMed: | PMC10493183 |
DOI: | 10.1093/molehr/gaad029 |
PMID: | 37610352 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1460-2407 |
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DOI: | 10.1093/molehr/gaad029 |