دورية أكاديمية
Probing the Biosafety of Implantable Artificial Secretory Granules for the Sustained Release of Bioactive Proteins.
العنوان: | Probing the Biosafety of Implantable Artificial Secretory Granules for the Sustained Release of Bioactive Proteins. |
---|---|
المؤلفون: | Álamo P; Institut d'Investigació Biomèdica Sant Pau (IIB SANT PAU), 08041 Barcelona, Spain.; Josep Carreras Leukaemia Research Institute (IJC), 08916 Badalona, Spain.; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN, ISCIII), Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain., Parladé E; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN, ISCIII), Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.; Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain., Favaro MTP; Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.; Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo 05508-000, Brazil., Gallardo A; Institut d'Investigació Biomèdica Sant Pau (IIB SANT PAU), 08041 Barcelona, Spain.; Department of Pathology, Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, Spain., Mendoza R; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN, ISCIII), Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.; Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain., Ferreira LCS; Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo 05508-000, Brazil., Roher N; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN, ISCIII), Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.; Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.; Department of Cell Biology, Animal Physiology and Immunology, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain., Mangues R; Institut d'Investigació Biomèdica Sant Pau (IIB SANT PAU), 08041 Barcelona, Spain.; Josep Carreras Leukaemia Research Institute (IJC), 08916 Badalona, Spain.; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN, ISCIII), Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain., Villaverde A; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN, ISCIII), Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.; Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.; Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain., Vázquez E; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN, ISCIII), Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.; Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.; Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain. |
المصدر: | ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2023 Aug 23; Vol. 15 (33), pp. 39167-39175. Date of Electronic Publication: 2023 Aug 10. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101504991 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1944-8252 (Electronic) Linking ISSN: 19448244 NLM ISO Abbreviation: ACS Appl Mater Interfaces Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Washington, D.C. : American Chemical Society |
مواضيع طبية MeSH: | Containment of Biohazards* , COVID-19*, Animals ; Humans ; Delayed-Action Preparations/pharmacology ; SARS-CoV-2 ; Prostheses and Implants ; Disease Models, Animal |
مستخلص: | Among bio-inspired protein materials, secretory protein microparticles are of clinical interest as self-contained, slow protein delivery platforms that mimic secretory granules of the human endocrine system, in which the protein is both the drug and the scaffold. Upon subcutaneous injection, their progressive disintegration results in the sustained release of the building block polypeptides, which reach the bloodstream for systemic distribution and subsequent biological effects. Such entities are easily fabricated in vitro by Zn-assisted cross-molecular coordination of histidine residues. Using cationic Zn for the assembly of selected pure protein species and in the absence of any heterologous holding material, these granules are expected to be nontoxic and therefore adequate for different clinical uses. However, such presumed biosafety has not been so far confirmed and the potential protein dosage threshold not probed yet. By selecting the receptor binding domain (RBD) from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein as a model protein and using a mouse lab model, we have explored the toxicity of RBD-made secretory granules at increasing doses up to ∼100 mg/kg of animal weight. By monitoring body weight and biochemical blood markers and through the histological scrutiny of main tissues and organs, we have not observed systemic toxicity. Otherwise, the bioavailability of the material was demonstrated by the induction of specific antibody responses. The presented data confirm the intrinsic biosafety of artificial secretory granules made by recombinant proteins and prompt their further clinical development as self-contained and dynamic protein reservoirs. |
References: | Clin Immunol. 2013 Dec;149(3):534-55. (PMID: 24263283) Adv Exp Med Biol. 2019;1174:265-290. (PMID: 31713202) Curr Genet. 2020 Oct;66(5):849-866. (PMID: 32356034) Adv Mater. 2020 Feb;32(7):e1907348. (PMID: 31879981) Toxicol Pathol. 1996 Jan-Feb;24(1):48-57. (PMID: 8839280) Acta Biomater. 2019 Jan 1;83:257-264. (PMID: 30366134) Nanomedicine (Lond). 2014 Dec;9(18):2817-28. (PMID: 25535685) Adv Mater. 2013 Jun 18;25(23):3144-76. (PMID: 23681931) J Control Release. 2001 Jun 15;73(2-3):121-36. (PMID: 11516493) J Pharm Sci. 2016 Feb;105(2):417-430. (PMID: 26869409) ACS Nano. 2021 Apr 27;15(4):5819-5837. (PMID: 33760579) Sci Rep. 2016 Oct 24;6:35765. (PMID: 27775083) Colloids Surf B Biointerfaces. 2020 Mar;187:110644. (PMID: 31757445) RSC Chem Biol. 2021 May 4;2(4):1004-1020. (PMID: 34458822) Pharmaceutics. 2022 Jan 14;14(1):. (PMID: 35057088) Nat Struct Mol Biol. 2020 Dec;27(12):1178-1184. (PMID: 33046908) Bioengineering (Basel). 2022 Mar 05;9(3):. (PMID: 35324796) J Exp Clin Cancer Res. 2022 Feb 4;41(1):49. (PMID: 35120582) Adv Sci (Weinh). 2019 Nov 27;7(3):1902420. (PMID: 32042562) Elife. 2022 Mar 08;11:. (PMID: 35257659) Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2022 Mar;14(2):e1756. (PMID: 34532989) Expert Opin Emerg Drugs. 2008 Mar;13(1):197-211. (PMID: 18321157) Adv Drug Deliv Rev. 2008 Jan 14;60(2):184-98. (PMID: 18045729) Sci Rep. 2016 Mar 23;6:23370. (PMID: 27004850) Science. 2009 Jul 17;325(5938):328-32. (PMID: 19541956) Life (Basel). 2020 Aug 21;10(9):. (PMID: 32825636) Materials (Basel). 2020 Nov 21;13(22):. (PMID: 33233413) Biotechnol Adv. 2022 Jan-Feb;54:107817. (PMID: 34418503) Mater Today (Kidlington). 2017 Apr;20(3):95-97. (PMID: 28522922) J Clin Pharmacol. 2009 Sep;49(9):1012-24. (PMID: 19620385) Chem Rev. 2023 Mar 8;123(5):2049-2111. (PMID: 36692900) Drug Deliv. 2021 Dec;28(1):2383-2391. (PMID: 34747685) AAPS J. 2006 Aug 04;8(3):E501-7. (PMID: 17025268) ACS Sustain Chem Eng. 2021 Sep 13;9(36):12341-12354. (PMID: 34603855) Pharmaceutics. 2023 Feb 22;15(3):. (PMID: 36986589) J Cell Mol Med. 2021 Nov;25(21):9905-9917. (PMID: 34626066) Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2021 May;13(3):e1684. (PMID: 33314628) Angew Chem Int Ed Engl. 2021 Jun 7;60(24):13158-13176. (PMID: 33145879) Trends Pharmacol Sci. 2010 Feb;31(2):53-9. (PMID: 19963283) Pharmaceutics. 2023 Apr 09;15(4):. (PMID: 37111682) Biotechnol Adv. 2022 Dec;61:108032. (PMID: 36089254) FEMS Microbiol Rev. 2019 Jan 1;43(1):53-72. (PMID: 30357330) Front Pharmacol. 2021 May 10;12:679602. (PMID: 34040536) Nanomaterials (Basel). 2020 Oct 05;10(10):. (PMID: 33027891) Biomater Sci. 2023 Mar 14;11(6):2200-2210. (PMID: 36745434) Acc Chem Res. 2016 Oct 18;49(10):2188-2198. (PMID: 27677734) Microorganisms. 2020 Dec 09;8(12):. (PMID: 33316961) J Clin Pharmacol. 2012 Jan;52(1):18-28. (PMID: 21233304) J Mater Chem B. 2023 Mar 1;11(9):1829-1848. (PMID: 36786439) Pharmaceutics. 2020 Dec 07;12(12):. (PMID: 33297493) J Control Release. 2018 Jun 10;279:29-39. (PMID: 29641987) J Control Release. 2018 Mar 28;274:81-92. (PMID: 29408658) Trends Microbiol. 2021 Mar;29(3):251-265. (PMID: 33041179) J Control Release. 2020 Nov 10;327:61-69. (PMID: 32768629) Trends Biochem Sci. 2020 Nov;45(11):992-1003. (PMID: 32891514) |
فهرسة مساهمة: | Keywords: drug delivery; protein engineering; protein materials; recombinant protein; self-assembling |
المشرفين على المادة: | 0 (Delayed-Action Preparations) |
تواريخ الأحداث: | Date Created: 20230824 Date Completed: 20230825 Latest Revision: 20230828 |
رمز التحديث: | 20230828 |
مُعرف محوري في PubMed: | PMC10450642 |
DOI: | 10.1021/acsami.3c08643 |
PMID: | 37614001 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1944-8252 |
---|---|
DOI: | 10.1021/acsami.3c08643 |