In Salmonella enterica, the pathogenicity island 2 (SPI-2) regulator PagR regulates its own expression and the expression of a five-gene operon that encodes transketolase C.

التفاصيل البيبلوغرافية
العنوان:	In Salmonella enterica, the pathogenicity island 2 (SPI-2) regulator PagR regulates its own expression and the expression of a five-gene operon that encodes transketolase C.
المؤلفون:	Parks AR; Department of Microbiology, University of Georgia, Athens, Georgia, USA., McCormick RD; Department of Microbiology, University of Georgia, Athens, Georgia, USA., Byrne JA; Department of Microbiology, University of Georgia, Athens, Georgia, USA., Escalante-Semerena JC; Department of Microbiology, University of Georgia, Athens, Georgia, USA.
المصدر:	Molecular microbiology [Mol Microbiol] 2023 Oct; Vol. 120 (4), pp. 575-586. Date of Electronic Publication: 2023 Aug 24.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 8712028 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2958 (Electronic) Linking ISSN: 0950382X NLM ISO Abbreviation: Mol Microbiol Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Oxford, OX ; Boston, MA : Blackwell Scientific Publications, c1987-
مستخلص:	The enteropathogen Salmonella enterica subsp. enterica sv. Typhimurium str. LT2 (hereafter S. Typhimurium) utilizes a cluster of genes encoded within the pathogenicity island 2 (SPI-2) of its genome to proliferate inside macrophages. The expression of SPI-2 is controlled by a complex network of transcriptional regulators and environmental cues, which now include a recently characterized DNA-binding protein named PagR. Growth of S. Typhimurium in low-phosphate, low-magnesium medium mimics conditions inside macrophages. Under such conditions, PagR ensures SPI-2 induction by upregulating the transcription of slyA, which encodes a known activator of SPI-2. Here, we report that PagR represses the expression of a divergently transcribed polycistronic operon that encodes the two subunits of transketolase TktC (i.e., tktD, tktE) of this bacterium. Transketolases contribute to the nonredox rearrangements of phosphorylated sugars of the pentose phosphate pathway, which provide building blocks for amino acids, nucleotides, cofactors, etc. We also demonstrate that PagR represses the expression of its own gene and define two PagR-binding sites between stm2344 and pagR. (© 2023 John Wiley & Sons Ltd.)
References:	Plasmid. 2016 Jul;86:1-6. (PMID: 27234933) Bioinformatics. 2018 Jun 1;34(11):1937-1938. (PMID: 29360956) Gene. 2002 May 15;290(1-2):153-61. (PMID: 12062810) Nucleic Acids Res. 2012 Nov 1;40(20):e156. (PMID: 22821568) Nucleic Acids Res. 2012 Aug;40(15):e115. (PMID: 22730293) Cell Microbiol. 2020 Feb;22(2):e13125. (PMID: 31610610) J Biol Chem. 2018 Jul 20;293(29):11271-11282. (PMID: 29848552) Methods. 2001 Dec;25(4):402-8. (PMID: 11846609) Curr Opin Microbiol. 2009 Apr;12(2):129-37. (PMID: 19269243) J Biol Chem. 1992 Aug 5;267(22):15869-74. (PMID: 1639817) Science. 2021 Jan 22;371(6527):400-405. (PMID: 33479153) J Bacteriol. 1968 Jul;96(1):215-20. (PMID: 4874308) Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5. (PMID: 10829079) Gene. 1984 Jul-Aug;29(1-2):247-50. (PMID: 6092227) Photosynth Res. 2003;75(1):1-10. (PMID: 16245089) Bioinformatics. 2007 May 15;23(10):1289-91. (PMID: 17379693) Biol Rev Camb Philos Soc. 2015 Aug;90(3):927-63. (PMID: 25243985) Appl Environ Microbiol. 1976 Dec;32(6):781-91. (PMID: 827241) Nucleic Acids Res. 2014 Jul;42(Web Server issue):W320-4. (PMID: 24753421) Biochim Biophys Acta. 1998 Jun 29;1385(2):387-98. (PMID: 9655943)
معلومات مُعتمدة:	R35 GM130399 United States GM NIGMS NIH HHS; R35-GM130399 United States GM NIGMS NIH HHS
فهرسة مساهمة:	Keywords: PagR protein; Salmonella pathogenesis; pagR gene expression; pentose phosphate pathway; tktDE expression; transcriptional regulation; transketolase
تواريخ الأحداث:	Date Created: 20230825 Latest Revision: 20231024
رمز التحديث:	20231024
مُعرف محوري في PubMed:	PMC10592175
DOI:	10.1111/mmi.15143
PMID:	37621115
قاعدة البيانات:	MEDLINE

View record at Wiley

Full Text Finder

الوصف
تدمد:	1365-2958
DOI:	10.1111/mmi.15143