دورية أكاديمية
أعرض في EDS

Chromogenic in situ hybridization technique for detecting porcine circovirus 3 in lung and lymphoid tissues.

التفاصيل البيبلوغرافية
العنوان: Chromogenic in situ hybridization technique for detecting porcine circovirus 3 in lung and lymphoid tissues.
المؤلفون: Tan CY; Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia., Lee KC; Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia., Chiou MT; Animal Disease Diagnostic Center, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan.; Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan., Lin CN; Animal Disease Diagnostic Center, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan.; Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan., Ooi PT; Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
المصدر: Veterinary world [Vet World] 2023; Vol. 16 (7), pp. 1444-1450. Date of Electronic Publication: 2023 Jul 09.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Veterinary World Country of Publication: India NLM ID: 101504872 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0972-8988 (Print) Linking ISSN: 09728988 NLM ISO Abbreviation: Vet World Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: Dist. Morbi (Gujarat), India : Veterinary World
Original Publication: Rajkot (Gujarat), India : Veterinary World, [2008]-
مستخلص: Background and Aim: Porcine circovirus 3 (PCV3) was recently reported in Malaysian commercial pig population in 2020 by conventional polymerase chain reaction (PCR), revealing a molecular prevalence of 17.02% in the sampled domestic pig population. This study aims to describe a chromogenic in situ hybridization (ISH) technique using digoxigenin (DIG)-labeled cloned PCV3 open reading frame 1 (ORF1) fragment DNA to detect and localize the PCV3 antigen in formalin-fixed, paraffin-embedded lung, and lymphoid tissue specimens.
Materials and Methods: Since PCV3 was mainly detected in lung and lymphoid tissues, we obtained tissue specimens from these organs from the previous Malaysian PCV3 study. Digoxigenin-labeled ISH probes were designed to target a 69 bp region of PCV3 ORF1 spanning from the nucleotide positions (282-350).
Results: Light microscopy analysis revealed that chromogenic staining of PCV3 antigens was visualized within the cytoplasm of pneumocytes and lymphocytes, indicating positive ISH results. The results of molecular detection of PCV3 using PCR and ISH showed a high agreement of 90.91%, including for the negative PCV3 status for all samples.
Conclusion: This study reports a chromogenic ISH technique using DIG-labeled probes targeting PCV3 ORF1 to detect PCV3 antigens in lung and lymphoid tissues. Despite the limited availability of PCV3 antibodies, ISH remains relevant for investigating PCV3 replication and pathogenesis and can be used complementarily with PCR for evaluating the localization of antigens in infected tissues.
Competing Interests: The authors declare that they have no competing interests.
(Copyright: © Tan, et al.)
References: Animals (Basel). 2022 Jul 21;12(14):. (PMID: 35883396)
Transbound Emerg Dis. 2022 Mar;69(2):538-548. (PMID: 33529468)
Vet Microbiol. 2004 Feb 4;98(2):81-8. (PMID: 14741119)
Front Vet Sci. 2018 Dec 12;5:315. (PMID: 30631769)
Front Vet Sci. 2019 Nov 15;6:403. (PMID: 31803766)
J Mol Biol. 1990 Oct 5;215(3):403-10. (PMID: 2231712)
Diagn Mol Pathol. 2003 Mar;12(1):1-13. (PMID: 12605030)
Animals (Basel). 2021 May 24;11(6):. (PMID: 34073660)
Transbound Emerg Dis. 2022 Sep;69(5):e1632-e1641. (PMID: 35240007)
Transbound Emerg Dis. 2020 May;67(3):1057-1061. (PMID: 31823481)
Vet Microbiol. 2020 Feb;241:108576. (PMID: 31928694)
Viruses. 2021 Feb 09;13(2):. (PMID: 33572209)
Prog Histochem Cytochem. 2016 Jan;50(4):37-48. (PMID: 26797255)
Infect Genet Evol. 2019 Sep;73:227-233. (PMID: 31096019)
Emerg Microbes Infect. 2019;8(1):684-698. (PMID: 31096848)
PLoS One. 2020 Jul 24;15(7):e0235832. (PMID: 32706778)
Vet Pathol. 2014 Jan;51(1):238-56. (PMID: 24129897)
Porcine Health Manag. 2022 Dec 5;8(1):51. (PMID: 36471405)
Sci Rep. 2023 Mar 30;13(1):5168. (PMID: 36997663)
Virol J. 2016 Nov 11;13(1):184. (PMID: 27835942)
Vet Pathol. 1998 May;35(3):159-67. (PMID: 9598579)
Vet Microbiol. 2018 Feb;215:71-76. (PMID: 29426409)
Vet J. 2004 Jul;168(1):41-9. (PMID: 15158207)
J Comp Pathol. 1999 Oct;121(3):265-70. (PMID: 10486162)
Mol Biol Evol. 2016 Jul;33(7):1870-4. (PMID: 27004904)
J Virol. 2019 Feb 5;93(4):. (PMID: 30487279)
Virus Res. 2022 Jun;314:198764. (PMID: 35367483)
J Virol. 2016 Dec 16;91(1):. (PMID: 27795441)
Res Vet Sci. 2001 Jun;70(3):265-9. (PMID: 11676625)
فهرسة مساهمة: Keywords: chromogenic in situ hybridization; in situ hybridization; pigs; porcine circovirus type 3; porcine circoviruses
تواريخ الأحداث: Date Created: 20230825 Latest Revision: 20230826
رمز التحديث: 20230826
مُعرف محوري في PubMed: PMC10446708
DOI: 10.14202/vetworld.2023.1444-1450
PMID: 37621535
قاعدة البيانات: MEDLINE
الوصف
تدمد:0972-8988
DOI:10.14202/vetworld.2023.1444-1450