دورية أكاديمية
أعرض في EDS

Comprehensive Molecular Evaluation of HNF-1 Alpha, miR-27a, and miR-146 Gene Variants and Their Link with Predisposition and Progression in Type 2 Diabetes Patients.

التفاصيل البيبلوغرافية
العنوان: Comprehensive Molecular Evaluation of HNF-1 Alpha, miR-27a, and miR-146 Gene Variants and Their Link with Predisposition and Progression in Type 2 Diabetes Patients.
المؤلفون: Mir R; Department of Medical Lab Technology, Prince Fahad Bin Sultan Chair for Biomedical Research, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk 71491, Saudi Arabia., Elfaki I; Department of Biochemistry, Faculty of Science, University of Tabuk, Tabuk 47512, Saudi Arabia., Elangeeb ME; Department of Basic Medical Sciences, Faculty of Applied Medical Sciences, University of Bisha, Bisha 67714, Saudia Arabia., Moawadh MS; Department of Medical Lab Technology, Prince Fahad Bin Sultan Chair for Biomedical Research, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk 71491, Saudi Arabia., Tayeb FJ; Department of Medical Lab Technology, Prince Fahad Bin Sultan Chair for Biomedical Research, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk 71491, Saudi Arabia., Barnawi J; Department of Medical Lab Technology, Prince Fahad Bin Sultan Chair for Biomedical Research, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk 71491, Saudi Arabia., Albalawi IA; Department of Surgical Oncology, Faculty of Medicine, University of Tabuk, Tabuk 47713, Saudi Arabia., Alharbi AA; Department of Biochemistry, Faculty of Science, University of Tabuk, Tabuk 47512, Saudi Arabia., Alhelali MH; Department of Statistics, University of Tabuk, Tabuk 47512, Saudi Arabia., Alsaedi BSO; Department of Statistics, University of Tabuk, Tabuk 47512, Saudi Arabia.
المصدر: Journal of personalized medicine [J Pers Med] 2023 Aug 17; Vol. 13 (8). Date of Electronic Publication: 2023 Aug 17.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101602269 Publication Model: Electronic Cited Medium: Print ISSN: 2075-4426 (Print) Linking ISSN: 20754426 NLM ISO Abbreviation: J Pers Med Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, 2011-
مستخلص: Background: Type 2 diabetes (T2D) is a metabolic condition induced by insulin resistance and pancreatic beta cell dysfunction. MicroRNAs (miRNAs) have biological significance because they regulate processes such as the molecular signaling pathways involved in the pathophysiology of diabetes mellitus. The hepatocyte nuclear factor-1 alpha (HNF-1 alpha) is a transcription factor found in hepatocytes and the pancreas. Mutations in the HNF-1 alpha gene were reportedly associated with maturity-onset diabetes of the young (MODY). The objective of the present study was to examine the associations between MiR-27a, MiR-146, and HNF-1 alpha single-nucleotide variations (SNVs) with T2D risk in the Saudi population.
Methodology: We evaluated the association of SNVs of miR-27a rs895819 A>G, 146a-rs2910164 C>G, and HNF-1 alpha rs1169288 G>T (I27L) with the risk of T2D in Saudi patients with the Amplification Refractory Mutation System PCR (ARMS-PCR). For the miR-27a SNVs, we used 115 cases (82 males, 33 females) and 117 matched healthy controls (HCs); for the Mir-146 SNVs, we used 103 cases (70 males, 33 females) and 108 matched HCs; and for the HNF-1 alpha, we employed 110 patients (80 males, 30 females) and 110 HCs. The blood biochemistry of the participants was essayed using commercial kits, and the methods of statistical analysis used were the Chi-square test, the Fisher exact test, and a multivariate analysis based on logistic regression, like the odds ratio (OD) and risk ratio (RR), with 95% confidence intervals (CIs).
Results: The MiR-27a rs895819 AG genotype was linked to increased T2D susceptibility, with OR = 2.01 and p -value = 0.011, and the miR-146 rs2910164 CG genotype and C allele were linked to an elevated risk of T2D, with OR = 2.75, p -value < 0.0016, OR = 1.77, and p -value = 0.004. The results also showed that the GT genotype and T allele of the HNF-1 alpha (rs1169288) G>T is linked to T2D, with OR = 2.18, p -value = 0.0061, and 1.77, p -value = 0.0059.
Conclusions: The SNVs in miR-27a, miR-146, and HNF-1 alpha can be potential loci for T2D risk. The limitations of this study include the relatively small sample size and the fact that it was a cross-sectional study. To our knowledge, this is the first study to highlight the association between miR-27a, miR-146, and HNF-1 alpha SNVs and the risk of T2D in the Saudi population. Future large-scale case-control studies, as well as studies on the functions of the proteins and protein interaction studies for HNF-1 alpha, are required to verify our findings. Furthermore, these findings can be used for the identification and stratification of at-risk populations via genetic testing for T2D-prevention strategies.
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فهرسة مساهمة: Keywords: Amplification Refractory Mutation System PCR (ARMS-PCR); hepatocytes nuclear factor-1 alpha (HNF-1 alpha); miR-146 rs2910164; miR-27a rs895819 SNV; microRNAs; single-nucleotide variations (SNVs); type 2 diabetes
تواريخ الأحداث: Date Created: 20230825 Latest Revision: 20230829
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC10455578
DOI: 10.3390/jpm13081270
PMID: 37623520
قاعدة البيانات: MEDLINE
الوصف
تدمد:2075-4426
DOI:10.3390/jpm13081270